Abstract
Genome sequence of Paenibacillus polymyxa ND25 isolated from cow rumen is reported for being a potential candidate in hydrolysis of lignocellulosic plant biomass. Draft genome sequence generated 5.73 Mb data containing 4922 putative protein coding genes, of which 140 are annotated for glycoside hydrolases. P. polymyxa ND25 strain comprises diverse lignocellulolytic components, especially 12 cellulase along with 23 hemicellulases and 11 esterases, signifying its potential for lignocellulose hydrolysis. Subsequent enzyme assay exhibited the potential of strain to produce 0.49, 0.24 and 0.44 U/ml U/ml of endoglucanase, exoglucanase and β-glucosidase, respectively, utilizing sugarcane bagasse as the sole carbon source. This study signifies the efficient application of P. polymyxa ND25 for facilitating plant-biomass utilization.
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Acknowledgements
Miss Varsha Bohra acknowledges Department of Science and technology (DST) of India for awarding Junior Research Fellowship (JRF). The funding from DBT project G-1-2282 is gratefully acknowledged for carrying out the work. The manuscript has been checked for plagiarism by Knowledge Resource Centre, CSIR-NEERI, Nagpur, India and assigned KRC No.: CSIR-NEERI/KRC/2018/April/EBGD/2.
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Accession numbers: The 16S rDNA gene sequence of P. polymyxa ND25 was deposited in the GenBank database under accession number KX404921. The whole genome shotgun sequence (WGS) of the strain ND25 was deposited in the NCBI-WGS database under accession number LZEL00000000. The accession numbers and locus tag of cellulase, hemicellulase and esterases are listed in Table 2.
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Bohra, V., Dafale, N.A. & Purohit, H.J. Paenibacillus polymyxa ND25: candidate genome for lignocellulosic biomass utilization. 3 Biotech 8, 248 (2018). https://doi.org/10.1007/s13205-018-1274-3
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DOI: https://doi.org/10.1007/s13205-018-1274-3