Fractionation of lignocellulosic biopolymers from sugarcane bagasse using formic acid-catalyzed organosolv process
- 103 Downloads
A one-step formic acid-catalyzed organosolv process using a low-boiling point acid–solvent system was studied for fractionation of sugarcane bagasse. Compared to H2SO4, the use of formic acid as a promoter resulted in higher efficiency and selectivity on removals of hemicellulose and lignin with increased enzymatic digestibility of the cellulose-enriched solid fraction. The optimal condition from central composite design analysis was determined as 40 min residence time at 159 °C using water/ethanol/ethyl acetate/formic acid in the respective ratios of 43:20:16:21%v/v. Under this condition, a 94.6% recovery of cellulose was obtained in the solid with 80.2% cellulose content while 91.4 and 80.4% of hemicellulose and lignin were removed to the aqueous–alcohol–acid and ethyl acetate phases, respectively. Enzymatic hydrolysis of the solid yielded 84.5% glucose recovery compared to available glucan in the raw material. Physicochemical analysis revealed intact cellulose fibers with decreased crystallinity while the hemicellulose was partially recovered as mono- and oligomeric sugars. High-purity organosolv lignin with < 1% sugar cross-contamination was obtained with no major structural modification according to Fourier-transform infrared spectroscopy. The work represents an alternative process for efficient fractionation of lignocellulosic biomass in biorefineries.
KeywordsBiorefinery Fractionation Organosolv Sugarcane bagasse Solvent recovery
This project was financially supported by PTT Global Chemicals and the Thailand Research Fund (RTA5980006). Manuscript proofreading by Dr. Pornkamol Unrean and Dr. Philip J. Shaw is appreciated.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Black SK, Hames BR, Myers MD (1998) Inventors. Midwest Research Institute, Assignee. Method of separating lignocellulosic material into lignin, cellulose and dissolved sugars, United State patent US 5730837, Mar 24Google Scholar
- Fengel D, Wegener G (1984) Wood: Chemistry. Ultrastructure, Reactions, De Gruyster, BerlinGoogle Scholar
- Hi KL, Yeap SP, Mashitah MD (2012) Pretreatment of pressed pericarp fibers (PPF) using alcohol as solvent to increase the accessibility of cellulose for cellulose production. Appl Biol Chem 55:507–514Google Scholar
- Li MF, Yu P, Li SX, Wu XF, Xiao X, Bian J (2017b) Sequential two-step fractionation of lignocellulose with formic acid organosolv followed by alkaline hydrogen peroxide under mild conditions to prepare easily saccharified cellulose and value-added lignin. Energy Convers Manage 148:1426–1437CrossRefGoogle Scholar
- McDonough TJ (1993) The chemistry of organosolv delignification. Tappi J 76:186–193Google Scholar
- Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008) Determination of sugars, byproducts, and degradation products in liquid fraction process samples. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory NREL/TP-510-42623Google Scholar
- Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2011) Determination of structural carbohydrates and lignin in biomass. Laboratory Analytical Procedure (LAP). National Renewable Energy Laboratory NREL/TP-510-42618Google Scholar
- Zhang K, Pei Z, Wang D (2016a) Organic solvent pretreatment of lignocellulosic biomass for biofuels and biochemical: a review. Bioresour Technol 199:106–114Google Scholar