Bioactive characteristics and optimization of tamarind seed protein hydrolysate for antioxidant-rich food formulations
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Tamarind seed has been a source of valuable nutrients such as protein (contains high amount of many essential amino acids), essential fatty acids, and minerals which are recognized as additive to develop perfect balanced functional foods. The objective of present work was to optimize the process parameters for extraction and hydrolysis of protein from tamarind seeds. Papain-derived hydrolysates showed a maximum degree of hydrolysis (39.49%) and radical scavenging activity (42.92 ± 2.83%) at optimized conditions such as enzyme-to-substrate ratio (1:5), hydrolysis time (3 h), hydrolysis temperature (65 °C), and pH 6. From this study, papain hydrolysate can be considered as good source of natural antioxidants in developing food formulations.
KeywordsTamarindus indica seed Papain-derived hydrolysates Scavenging Reducing power
The authors gratefully acknowledge Advance Enzyme, Mumbai, India for providing free samples of enzyme used in the study. The authors also thank the Department of Biotechnology (DBT), Government of India for financial support to carry out this work.
Compliance with ethical standards
Conflict of interest
Authors do not have any conflict of interest.
- AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Washington, DCGoogle Scholar
- Bagul MB, Sonawane SK, Arya SS (2015) Tamarind seeds: chemistry, technology, applications and health benefits: a review. Indian Food Ind 34(3):28–35Google Scholar
- Chaplin MF, Bucke C (1990) Enzyme technology. CUP Arch 1990:20Google Scholar
- De Lumen BO, Becker R, Reyes PS (1986) The nutritive value of tamarind seed for broiler chicks. Anim Feed Sci Technol 22(4):285–293Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randal RJ (1951) Protein analysis with Folin-phenol reagent. J Biol Chem 193:265–275Google Scholar
- Page S (1985) Characterization of Oat (Avena sativa L.) residual proteins. Cereal Chem 62(4):276–279Google Scholar
- Pelegrini PB, Murad AM, Silva LP, dos Santos RCP, Costa FT, Tagliari PD, Bloch C Jr, Noronha EF, Miller RNG, Franco OL (2008) Identification of a novel storage glycine-rich peptide from guava (Psidium guajava) seeds with activity against Gram-negative bacteria. Peptides 29:1271–1279CrossRefGoogle Scholar
- Singh D, Wangchu L, Moond SK (2007) Processed products of Tamarind. Nat Prod Rad 6(4):315–321Google Scholar
- Tsopmo A, Cooper A, Jodayree S (2010) Enzymatic hydrolysis of oat flour protein isolates to enhance antioxidative properties. Adv J Food Sci Technol 2(4):206–212Google Scholar