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3 Biotech

, 8:213 | Cite as

High-titer and productivity of l-(+)-lactic acid using exponential fed-batch fermentation with Bacillus coagulans arr4, a new thermotolerant bacterial strain

  • Luciana Fontes Coelho
  • Susan Michelz Beitel
  • Daiane Cristina Sass
  • Paulo Marcelo Avila Neto
  • Jonas Contiero
Original Article

Abstract

Bacillus coagulans arr4 is a thermotolerant microorganism with great biotechnological potential for l-(+)-lactic acid production from granulated sugar and yeast extract. The highest l-(+)-lactic acid production was obtained with Ca(OH)2. The maximum production of l-(+)-lactic acid (206.81 g/L) was observed in exponential feeding using granulated sugar solution (900 g/L) and yeast extract (1%) at 50 °C, pH 6.5, and initial granulated sugar concentration of 100 g/L at 39 h. 5.3 g/L h productivity and 97% yield were observed, and no sugar remained. Comparing the simple batch with exponential fed-batch fermentation, the l(+) lactic acid production was improved in 133.22% and dry cell weight was improved in 83.29%, using granulated sugar and yeast extract. This study presents the highest productivity of lactic acid ever observed in the literature, on the fermentation of thermotolerant Bacillus sp. as well as an innovative and high-efficiency purification technology, using low-cost substances as Celite and charcoal. The recovery of lactic acid was 86%, with 100% protein removal, and the fermentation medium (brown color) became a colorless solution.

Keywords

Bacillus sp. l(+) lactic acid Fed-batch Thermotolerance Purification 

Notes

Acknowledgements

This work was financed by Fapesp (Grant no. 2010/52416-8), Braskem (Grant no. 2010/52416-8b) and CNPq (Grant no. 301567/2012-3).

Supplementary material

13205_2018_1232_MOESM1_ESM.xlsx (12 kb)
Supplementary material 1 (XLSX 12 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Luciana Fontes Coelho
    • 1
  • Susan Michelz Beitel
    • 1
  • Daiane Cristina Sass
    • 1
  • Paulo Marcelo Avila Neto
    • 1
  • Jonas Contiero
    • 1
    • 2
  1. 1.Department of Biochemistry and Microbiology, Biological Sciences InstituteSão Paulo State University (UNESP)Rio ClaroBrazil
  2. 2.São Paulo State University (UNESP), Bioenergy Research Institute (IPBEN)Rio ClaroBrazil

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