Extraction and characterization of collagen hydrolysates from the skin of Rana chensinensis
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The production of Hasma generates plentiful non-edible by-products in China and Central Asia. As one of main by-products, the skin of Rana chensinensis is discarded as waste without utilization. In this work, R. chensinensis skin collagen (RCSC) hydrolysates were extracted using pepsin under acidic conditions. The yield of RCSC is 15.1% (w/w). Amino acid analysis revealed that RCSC contained glycine (204.5/1000 residues) and imino acids (182/1000 residues). RCSC exhibited high solubility in acidic pH (1–4) and low NaCl concentrations (< 2%, w/v). Differential scanning calorimetry indicated that the denaturation temperature of RCSC was 33.5 °C. Scanning electron microscopy analysis confirmed their well-defined fibril morphologies. The results indicated that the skin of R. chensinensis is an alternative source of collagen hydrolysates, and RCSC can serve as a potential source applying in foodstuff and medical industry.
KeywordsRana chensinensis Skin Collagen hydrolysates Extraction Characterization
The authors gratefully acknowledge the financial supports by Program for Liaoning Excellent Talents in University (Grant no. LJQ2014040) and Science and Technology Development Foundation of Fushun City (Grant no. 20142101).
Compliance with ethical standards
Conflict of interest
All the authors declare that they have no conflict of interest.
- Foegeding EA, Lanier TC, Hultin HO (1996) Collagen. In: Fennema OR (ed) Food chemistry. Marcel Dekker, New York, pp 902–906Google Scholar
- Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Bio Chem 193:265–275Google Scholar
- Uriarte-Montoya MH, Arias-Moscoso JL, Plascencia-Jatomea M, Santacruz-Ortega H, Rouzaud-Sández O, Cardenas-Lopez JL, Marquez-Rios E, Ezquerra-Brauer JM (2010) Jumbo squid (Dosidicus gigas) mantle collagen: extraction, characterization, and potential application in the preparation of chitosan–collagen biofilms. Bioresour Technol 101:4212–4219CrossRefGoogle Scholar
- Zhu R, Shi S (2013) Collagen extracted from fresh skin of Rana chensinensis by acetic acid and pepsin successively. J Yanbian Univ (Nat Sci Ed) 39:202–205Google Scholar