Advertisement

3 Biotech

, 8:165 | Cite as

Optimization of the fermentation conditions for the mutant strain of β-cyclodextrin glycosyltransferase H167C to produce cyclodextrins

  • Hua Wang
  • Wenxi Zhou
  • Hua Li
  • Rie Bu
Original Article
  • 47 Downloads

Abstract

The cyclodextrin glycosyltransferase (CGTase) was used to catalyze the conversion of starch into cyclodextrins (CD) in industry. Improving the activity of CGTase to produce more CD with relative low cost is intensely interesting and has drawn wide attention. Amino acid mutation of His167 into Cys significantly enhanced β-CGTase activity; however, optimization of culture conditions for β-CGTase-H167C remains unclear. To determine this, the medium and culture conditions for β-CGTase-H167C were optimized with response surface methodology. Maximum activity of β-CGTase-H167C was obtained with the medium containing 1.1% corn starch, 4.4% corn steep liquor, 1.1% peptone, 0.02% MgSO4·7H2O and 0.1% K2HPO4·3H2O that were cultured with the initial pH 8.4, incubation temperature at 37.4 °C, with 5% inoculation size and shaking speed at 202 r/min. Under the optimal conditions, the activity of β-CGTase-H167C was up to 4355 U/mL, which is 1.93-fold in comparison with the initial activity. Our results established the promising culture strategy for the production of cyclodextrins by β-CGTase-H167C.

Keywords

β-CGTase Optimization Medium components Culture conditions Cyclodextrins 

Notes

Acknowledgements

The work was supported by Natural Science Foundation of Inner Mongolia (2015BS0312). The sponsors of financial support have no involvement in the study design, data collection and analysis, writing of the report and decision to submit the article for publication.

Compliance with ethical standards

Conflicts of interest

The authors declare that there is no conflict of interest regarding the publication of the paper.

References

  1. Abd Rahman R, Illias RM, Nawawi MGM, Ismail AF, Hassan O, Kamaruddin K (2004) Optimisation of growth medium for the production of cyclodextrin glucanotransferase from Bacillus stearothermophilus HR1 using response surface methodology. Process Biochem 39:2053–2060CrossRefGoogle Scholar
  2. Akindahunsi AA, Oboh G (2003) Effect of fungi fermentation on organoleptic properties, energy content and in vitro multienzyme digestibility of cassava products (flour and gari). Nutr Health 17:131–138.  https://doi.org/10.1177/026010600301700204 CrossRefGoogle Scholar
  3. Alves-Prado HF, Carneiro AA, Pavezzi FC, Gomes E, Boscolo M, Franco CM, da Silva R (2008) Production of cyclodextrins by CGTase from Bacillus clausii using different starches as substrates. Appl Biochem Biotechnol 146:3–13.  https://doi.org/10.1007/s12010-007-8093-z CrossRefGoogle Scholar
  4. Avci A, Donmez S (2009) A novel thermophilic anaerobic bacteria producing cyclodextrin glycosyltransferase. Process Biochem 44:36–42CrossRefGoogle Scholar
  5. Brauman A, Keleke S, Malonga M, Miambi E, Ampe F (1996) Microbiological and biochemical characterization of cassava retting, a traditional lactic Acid fermentation for foo-foo (cassava flour) production. Appl Environ Microbiol 62:2854–2858Google Scholar
  6. Coelho SL, Magalhaes VC, Marbach PA, Cazetta ML (2016) A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour. Braz J Microbiol 47:120–128.  https://doi.org/10.1016/j.bjm.2015.11.018 CrossRefGoogle Scholar
  7. Ebadipour N, Lotfabad TB, Yaghmaei S, RoostaAzad R (2016) Optimization of low-cost biosurfactant production from agricultural residues through response surface methodology. Prep Biochem Biotechnol 46:30–38.  https://doi.org/10.1080/10826068.2014.979204 CrossRefGoogle Scholar
  8. Gawande BN, Singh RK, Chauhan AK, Goel A, Patkar AY (1998) Optimization of cyclomaltodextrin glucanotransferase production from Bacillus firmus. Enzyme Microb Tech 22:288–291CrossRefGoogle Scholar
  9. He Q et al (2017) Production of chlorothalonil hydrolytic dehalogenase from agro-industrial wastewater and its application in raw food cleaning. J Sci Food Agric 97:2582–2587.  https://doi.org/10.1002/jsfa.8079 CrossRefGoogle Scholar
  10. Ibrahim HM, Yusoff WMW, Hamid AA, Illias RM, Hassan O, Omar O (2005) Optimization of medium for the production of beta-cyclodextrin glucanotransferase using central composite design (CCD). Process Biochem 40:753–758CrossRefGoogle Scholar
  11. Kimura K, Ishii Y, Kataoka S, Takano T, Yamane K (1990) Expression of the beta-cyclodextrin glucanotransferase gene of an alkalophilic Bacillus sp. #1011 in Escherichia coli cells and characterization of the synthesized enzyme. Agric Biol Chem 54:641–648Google Scholar
  12. Lee MH, Yang SJ, Kim JW, Lee HS, Kim JW, Park KH (2007) Characterization of a thermostable cyclodextrin glucanotransferase from Pyrococcus furiosus DSM3638. Extremophiles 11:537–541.  https://doi.org/10.1007/s00792-007-0061-6 CrossRefGoogle Scholar
  13. Lepiz-Aguilar L, Rodriguez-Rodriguez CE, Arias ML, Lutz G (2013) Acetone–Butanol–Ethanol (ABE) production in fermentation of enzymatically hydrolyzed cassava flour by Clostridium beijerinckii BA101 and solvent separation. J Microbiol Biotechnol 23:1092–1098CrossRefGoogle Scholar
  14. Lindner K, Saenger W (1980) Crystal structure of the gamma-cyclodextrin n-propanol inclusion complex; correlation of α-, β-, γ- cyclodextrin geometries. Biochem Biophys Res Commun 92:933–938CrossRefGoogle Scholar
  15. Ong RM, Goh KM, Mahadi NM, Hassan O, Rahman RN, Illias RM (2008) Cloning, extracellular expression and characterization of a predominant β-CGTase from Bacillus sp. G1 in E. coli. J Ind Microbiol Biotechnol 35:1705–1714.  https://doi.org/10.1007/s10295-008-0462-2 CrossRefGoogle Scholar
  16. Pinto FS, Flores SH, Ayub MA, Hertz PF (2007) Production of cyclodextrin glycosyltransferase by alkaliphilic Bacillus circulans in submerged and solid-state cultivation. Bioprocess Biosyst Eng 30:377–382.  https://doi.org/10.1007/s00449-007-0134-z CrossRefGoogle Scholar
  17. Rendleman JA Jr, Knutson CA Jr (1998) Conversion of cyclodextrin into high-amylose starch of low molecular mass by means of cyclodextrin glucanotransferase. Biotechnol Appl Biochem 28(Pt 3):219–228Google Scholar
  18. Rosso AM, Ferrarotti SA, Krymkiewicz N, Nudel BC (2002) Optimisation of batch culture conditions for cyclodextrin glucanotransferase production from Bacillus circulans DF 9R. Microb Cell Fact 1:3CrossRefGoogle Scholar
  19. Terada Y, Yanase M, Takata H, Takaha T, Okada S (1997) Cyclodextrins are not the major cyclic alpha-1,4-glucans produced by the initial action of cyclodextrin glucanotransferase on amylose. J Biol Chem 272:15729–15733CrossRefGoogle Scholar
  20. Wischral D et al (2016) Production of 1,3-propanediol by Clostridium beijerinckii DSM 791 from crude glycerol and corn steep liquor: process optimization and metabolic engineering. Bioresour Technol 212:100–110.  https://doi.org/10.1016/j.biortech.2016.04.020 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life ScienceInner Mongolia University for NationalitiesTongliaoChina
  2. 2.TongLiao Academy of Agricultural SciencesTongliaoChina

Personalised recommendations