An alkaline bacterial laccase for polymerization of natural precursors for hair dye synthesis
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In the present study, an extracellular alkali stable laccase (Lac DS) from Bacillus subtilis DS which has pH optima at 8.5 using p-phenylenediamine (PPD) as substrate has been reported. Lac DS retained 70% activity for 4 h at pH 8.5 and 90% activity for 24 h at 55 °C. The enzyme yield was enhanced by optimization of fermentation conditions. A 746-fold increase in yield was observed under optimized conditions using 150 µM MgSO4, 1.2% yeast extract, 0.35% tryptone, and 150 µM vanillic acid. Lac DS was used to polymerize natural dye precursor catechol, pyrogallol, syringaldehyde, syringic acid, ferulic acid and gallic acid to develop a range of natural hair colors such as black, golden yellow, and reddish brown. The results indicate that alkaline Lac DS is a suitable candidate to develop a user-friendly and commercially applicable hair dyeing process in the area of cosmetic industry.
KeywordsLaccase Hair dye Synthesis Polymerization PPD Natural precursors
This work was supported by University Grant Commission (UGC)-BSR F.25-1/2013-14(BSR)/7-261/2011 (BSR) Government of India, New Delhi, India.
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Conflict of interest
The authors declare that they have no conflict of interest
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Research involving Human Participants and/or Animals/Informed consent: not applicable for the study.
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