3 Biotech

, 8:154 | Cite as

Indigenous plant growth-promoting bacteria enhance plant growth, biomass, and nutrient uptake in degraded forest plants

  • Parthasarathy Radhapriya
  • Andimuthu Ramachandran
  • Perumal Palani
Original Article


The present study was aimed to isolate indigenous plant growth-promoting bacteria (PGPB) from Nanmangalam reserve forest, India and to analyze their positive impact on nursery plant species. In total, 160 isolates were obtained from different nitrogen-free Media (LGI, JMV, NFB). Amongst these, 12 isolates were shown positive for 5–8% of ammonia production nif H positive and then isolates were further tested for their plant growth-promoting (PGP) activity. Based on their PGP activity, nine isolates were selected, and applied in nurseries of twelve native plant species, along with organic manure and inorganic fertilizer. All the isolates were shown positive effects when compared to control. In that, five of these bacterial isolates, Paenibacillus sp. RRB2, Azospirillum brasilense RRAK5, Bacillus subtilis subsp. subtilis RRD8, Burholdria kururiensis RRAK1, and Pseudomonas stutzeri RRAN2, enhanced biomass production in several trees.


Indigenous PGPB Native plants Biomass Nutrient content 



The first author (PR) gratefully acknowledges the University Grants Commission (UGC-BSR), Government of India, UGC Sanction./-7/2007 (BSR), dated 16.11.2009 for providing the financial support for this research work. Authors thank the Tamil Nadu Forest Department for allowing nursery treatments in Nanmangalam Reserve Forest. We are extremely grateful to Forest Department staff for valuable assistance and continuous support during the study.

Author contributions

Conception and design of experiments: AR, PR. Manuscript preparation: PR, AR, PP. Data analysis: PR.

Compliance with ethical standards

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Parthasarathy Radhapriya
    • 1
    • 2
  • Andimuthu Ramachandran
    • 1
  • Perumal Palani
    • 2
  1. 1.Centre for Climate Change and Adaptation ResearchAnna UniversityChennaiIndia
  2. 2.Centre for Advanced Studies in BotanyUniversity of MadrasChennaiIndia

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