Optimization of extraction parameters of pentacyclic triterpenoids from Swertia chirata stem using response surface methodology

Abstract

In the present investigation, pentacyclic triterpenoids were extracted from different parts of Swertia chirata by solid–liquid reflux extraction methods. The total pentacyclic triterpenoids (UA, OA, and BA) in extracted samples were determined by HPTLC method. Preliminary studies showed that stem part contains the maximum pentacyclic triterpenoid and was chosen for further studies. Response surface methodology (RSM) has been employed successfully by solid–liquid reflux extraction methods for the optimization of different extraction variables viz., temperature (X1 35–70 °C), extraction time (X2 30–60 min), solvent composition (X3 20–80%), solvent-to-solid ratio (X4 30–60 mlg−1), and particle size (X5 3–6 mm) on maximum recovery of triterpenoid from stem parts of Swertia chirata. A Plackett–Burman design has been used initially to screen out the three extraction factors viz., particle size, temperature, and solvent composition on yield of triterpenoid. Moreover, central composite design (CCD) was implemented to optimize the significant extraction parameters for maximum triterpenoid yield. Three extraction parameters viz., mean particle size (3 mm), temperature (65 °C), and methanol–ethyl acetate solvent composition (45%) can be considered as significant for the better yield of triterpenoid A second-order polynomial model satisfactorily fitted the experimental data with the R2 values of 0.98 for the triterpenoid yield (p < 0.001), implying good agreement between the experimental triterpenoid yield (3.71%) to the predicted value (3.79%).

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References

  1. Alberti A, Zielinski AA, Zardo DM, Demiate IM, Nogueira A, Mafra LI (2014) Optimisation of the extraction of phenolic compounds from apples using response surface methodology. Food Chem 149:151–158

    CAS  Article  Google Scholar 

  2. Ameer K, Bae SW, Jo Y, Lee HG, Ameer A, Kwon JH (2017) Optimization of microwave-assisted extraction of total extract, stevioside and rebaudioside-A from Stevia rebaudiana (Bertoni) leaves, using response surface methodology (RSM) and artificial neural network (ANN) modelling. Food Chem 229:198–207

    CAS  Article  Google Scholar 

  3. Anonymous (1976) The wealth of India—a dictionary of Indian raw materials and industrial products. Raw materials. New Delhi: Publications and Information Directorate, Council of Scientific and Industrial Research. 10 (Sp–W), pp78–81

  4. Bai XL, Yue TL, Yuan YH, Zhang HW (2010) Optimization of microwave-assisted extraction of polyphenols from apple pomace using response surface methodology and HPLC analysis. J Sep Sci 33(23–24):3751–3758

    CAS  Article  Google Scholar 

  5. Banik RM, Pandey DK (2008) Optimizing conditions for oleanolic acid extraction from Lantana camara roots using response surface methodology. Ind Crops Prod 27(3):241–248

    CAS  Article  Google Scholar 

  6. Bhandari P, Gupta A, Singh B, Kaul V (2006) HPTLC determination of swertiamarin and amarogentin in Swertia species from the Western Himalayas. J Planar Chromatogr 19(109):212–215

    CAS  Article  Google Scholar 

  7. Bonaccorsi I, Altieri F, Sciamanna I, Oricchio E, Grillo C, Contartese G, Galati EM (2008) Endogenous reverse transcriptase as a mediator of ursolic acid’s anti-proliferative and differentiating effects in human cancer cell lines. Cancer Lett 263(1):130–139

    CAS  Article  Google Scholar 

  8. Cheok CY, Chin NL, Yusof YA, Talib RA, Law CL (2012) Optimization of total phenolic content extracted from Garcinia mangostana Linn. hull using response surface methodology versus artificial neural network. Ind Crops Prod 40:247–253

    CAS  Article  Google Scholar 

  9. Fang XJ, Wang XY, Zhang G, Zhao J (2010) Optimization of microwave-assisted extraction followed by RP-HPLC for the simultaneous determination of oleanolic acid and ursolic acid in the fruits of Chaenomeles sinensis. J Sep Sci 33:1147–1155

    CAS  Google Scholar 

  10. Gad HA, El-Ahmady SH, Abou-Shoer MI, Al-Azizi MM (2013) Application of chemometrics in authentication of herbal medicines: a review. Phytochem Anal 24(1):1–24

    CAS  Article  Google Scholar 

  11. Gao R, Wang L, Yang Y, Ni J, Zhao L, Dong S, Guo M (2015) Simultaneous determination of oleanolic acid, ursolic acid, quercetin and apigenin in Swertia mussotii Franch by capillary zone electrophoresis with running buffer modifier. Biomed Chromatogr 29(3):402–409

    Article  Google Scholar 

  12. Gheorgheosu D, Duicu O, Dehelean C, Soica C, Muntean D (2014) Betulinic acid as a potent and complex antitumor phytochemical: a minireview. Anticancer Agents Med Chem 14(7):936–945

    CAS  Article  Google Scholar 

  13. Gopal V, Mandal V, Mandal SC (2014) HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis. J Acute Dis 3(1):59–61

    Article  Google Scholar 

  14. Gupta M, Bisht D, Khatoon S, Srivastava S, Rawat AK (2011) Determination of ursolic acid a biomarker in different Swertia species through high performance thin layer chromatography. Chin Med 2(04):121

    Article  Google Scholar 

  15. Ilaiyaraja N, Likhith KR, Babu GS, Khanum F (2015) Optimisation of extraction of bioactive compounds from Feronia limonia (wood apple) fruit using response surface methodology (RSM). Food Chem 173:348–354

    CAS  Article  Google Scholar 

  16. Jacob S, Banerjee R (2016) Modeling and optimization of anaerobic codigestion of potato waste and aquatic weed by response surface methodology and artificial neural network coupled genetic algorithm. Bioresource Technol 214:386–395

    CAS  Article  Google Scholar 

  17. Jesus JA, Lago JH, Laurenti MD, Yamamoto ES, Passero LF (2015) Antimicrobial activity of oleanolic and ursolic acids: an update. Evid Based Complement Alternat Med 2015

  18. Khanal S, Shakya N, Nepal N, Pant D (2014) Swertia chirayita: the himalayan herb. Int J Appl Sci Biotechnol 2(4):389–392

    Google Scholar 

  19. Kshirsagar PR, Pai SR, Nimbalkar MS, Gaikwad NB (2015) Quantitative determination of three pentacyclic triterpenes from five Swertia L. species endemic to Western Ghats, India, using RP-HPLC analysis. Nat Prod Res 29(19):1783–1788

    CAS  Article  Google Scholar 

  20. Kumar V, Van Staden J (2015) A review of Swertia chirayita (Gentianaceae) as a traditional medicinal plant. Front Pharmacol 6:308

    Article  Google Scholar 

  21. Kumar KS, Bhowmik D, Chandira M (2010) Swertia chirayita: a traditional herb and its medicinal uses. J Chem Pharm Res 2(1):262–266

    Google Scholar 

  22. Li G, Zhang X, You J, Song C, Sun Z, Xia L, Suo Y (2011) Highly sensitive and selective pre-column derivatization high-performance liquid chromatography approach for rapid determination of triterpenes oleanolic and ursolic acids and application to Swertia species: optimization of triterpenic acids extraction and pre-column derivatization using response surface methodology. Anal Chim Acta 688(2):208–218

    CAS  Article  Google Scholar 

  23. Liang J, Ito Y, Zhang X, He J, Sun W (2013) Rapid preparative separation of six bioactive compounds from Gentiana crassicaulis Duthie ex Burk. using microwave-assisted extraction coupled with high-speed counter-current chromatography. J Sep Sci 36(24):3934–3940

    CAS  Article  Google Scholar 

  24. Liu J (1995) Pharmacology of oleanolic acid and ursolic acid. J Ethnopharmacol 49(2):57–68

    CAS  Article  Google Scholar 

  25. Mahendran G, Bai VN (2014) Micropropagation, antioxidant properties and phytochemical assessment of Swertia corymbosa (Griseb.) Wight ex CB Clarke: a medicinal plant. Acta Physiol Plant 36(3):589–603

    CAS  Article  Google Scholar 

  26. Negi JS, Singh P, Rawat B (2011) Chemical constituents and biological importance of Swertia: a review. Curr Res Chem 3(1):1–15

    CAS  Article  Google Scholar 

  27. Pandey DK, Basu S, Jha TB (2012) Screening of different East Himalayan species and populations of Swertia L. based on exomorphology and mangiferin content. Asian Pac J Tropical Biomed 2(3):S1450–S1456

    Article  Google Scholar 

  28. Paul GC, Kent CA, Thomas CR (1992) Quantitative characterization of vacuolization in Penicillium chrysogenum using automatic image analysis. Trans Inst Chem Eng C 70:13–20

    CAS  Google Scholar 

  29. Samaddar T, Chaubey B, Jha S, Jha TB (2013) Determination of swertiamarin and amarogentin content and evaluation of antibacterial activity in Eastern Himalayan species of Swertia L. Pharmacogn 3(4):64–70

    Google Scholar 

  30. Sethiya NK, Mishra S (2015) Simultaneous HPTLC analysis of ursolic acid, betulinic acid, stigmasterol and lupeol for the identification of four medicinal plants commonly available in the indian market as Shankhpushpi. J Chromatogr Sci 53(5):816–823

    CAS  Article  Google Scholar 

  31. Sheng ZL, Wan PF, Dong CL, Li YH (2013) Optimization of total flavonoids content extracted from Flos Populi using response surface methodology. Ind Crops Prod 43:778–786

    CAS  Article  Google Scholar 

  32. Silva FSG, Oliveira PJ, Duarte MF (2016) Oleanolic, ursolic, and betulinic acids as food supplements or pharmaceutical agents for type 2 diabetes: promise or Illusion? J Agric Food Chem 64(15):2991–3008

    CAS  Article  Google Scholar 

  33. Soica C, Oprean C, Borcan F, Danciu C, Trandafirescu C, Coricovac D, Crăiniceanu Z, Dehelean CA, Munteanu M (2014) The synergistic biologic activity of oleanolic and ursolic acids in complex with hydroxypropyl-γ-cyclodextrin. Molecules 19(4):4924–4940

    CAS  Article  Google Scholar 

  34. Verma SC, Jain CL, Nigam S, Padhi MM (2013) Rapid extraction, isolation, and quantification of oleanolic acid from Lantana camara L. Roots using microwave and HPLC-PDA techniques. Acta Chromatogr 25(1):181–199

    Article  Google Scholar 

  35. Wang C, Wang Y, Zhang J, Wang Z (2014) Optimization for the extraction of polysaccharides from Gentiana scabra Bunge and their antioxidant in vitro and anti-tumor activity in vivo. J Taiwan Inst Chem Eng 45(4):1126–1132

    CAS  Article  Google Scholar 

  36. Wójciak-Kosior M (2007) Separation and determination of closely related triterpenic acids by high performance thin-layer chromatography after iodine derivatization. J Pharmaceut Biomed Anal 45(2):337–340

    Article  Google Scholar 

  37. Yang YC, Wei MC, Hong SJ, Huang TC, Lee SZ (2013) Development/optimization of a green procedure with ultrasound-assisted improved supercritical carbon dioxide to produce extracts enriched in oleanolic acid and ursolic acid from Scutellaria barbata D. Don. Ind Crops Prod 49:542–553

    Article  Google Scholar 

  38. Yogeeswari P, Sriram D (2005) Betulinic acid and its derivatives: a review on their biological properties. Curr Med Chem 12(6):657–666

    CAS  Article  Google Scholar 

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Acknowledgements

The authors are grateful to the IPLS-DBT Project (Project No. BT/PR-4548/INF/22/146/2012) sanctioned to Punjabi University, Patiala for providing the facilities to carry out the present work.

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Correspondence to Devendra Kumar Pandey.

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Pandey, D.K., Kaur, P. Optimization of extraction parameters of pentacyclic triterpenoids from Swertia chirata stem using response surface methodology. 3 Biotech 8, 152 (2018). https://doi.org/10.1007/s13205-018-1174-6

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Keywords

  • Swertia chirata
  • Pentacyclic triterpenoids
  • RSM
  • Solid–liquid extraction
  • HPTLC