3 Biotech

, 8:164 | Cite as

Prospecting Ammoniphilus sp. JF isolated from agricultural fields for butachlor degradation

  • Jatinder Singh
  • Yogalakshmi Kadapakkam Nandabalan
Original Article


Butachlor is a chloroacetamide herbicide used worldwide for controlling weeds in plants of rice, corn, soybean and other crops. In this study, indigenous bacterial species Ammoniphilus sp. JF was isolated from the agricultural fields of Punjab and identified using 16S ribosomal RNA analysis. The bacteria utilized butachlor as the sole carbon source and showed complete degradation (100 mg/L) within 24 h of incubation. Two intermediate products, namely 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester and 2,4-bis(1,1-dimethylethyl)-phenol were observed at the end of butachlor degradation. To the best of author’s knowledge, biodegradation of butachlor by indigenous Ammoniphilus sp. JF from the agricultural fields of Punjab has not been reported so far.


Butachlor Herbicide Ammoniphilus sp. JF Indigenous 16S rRNA 



This work was supported by University Grant Commission (UGC) for providing financial support. Authors are thankful to central instrumentation laboratory of National Institute of Pharmaceutical Education and Research (NIPER), Mohali, Punjab, for providing GC–MS analysis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jatinder Singh
    • 1
  • Yogalakshmi Kadapakkam Nandabalan
    • 1
  1. 1.Centre for Environmental Science and Technology, School of Environment and Earth SciencesCentral University of PunjabBathindaIndia

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