Control of corrosive bacterial community by bronopol in industrial water system
- 42 Downloads
Ten aerobic corrosive bacterial strains were isolated from a cooling tower water system (CWS) which were identified based on the biochemical characterization and 16S rRNA gene sequencing. Out of them, dominant corrosion-causing bacteria, namely, Bacillus thuringiensis EN2, Terribacillus aidingensis EN3, and Bacillus oleronius EN9, were selected for biocorrosion studies on mild steel 1010 (MS) in a CWS. The biocorrosion behaviour of EN2, EN3, and EN9 strains was studied using immersion test (weight loss method), electrochemical analysis, and surface analysis. To address the corrosion problems, an anti-corrosive study using a biocide, bronopol was also demonstrated. Scanning electron microscopy and Fourier-transform infrared spectroscopy analyses of the MS coupons with biofilm developed after exposure to CWS confirmed the accumulation of extracellular polymeric substances and revealed that biofilms was formed as microcolonies, which subsequently cause pitting corrosion. In contrast, the biocide system, no pitting type of corrosion, was observed and weight loss was reduced about 32 ± 2 mg over biotic system (286 ± 2 mg). FTIR results confirmed the adsorption of bronopol on the MS metal surface as protective layer (co-ordination of NH2–Fe3+) to prevent the biofilm formation and inhibit the corrosive chemical compounds and thus led to reduction of corrosion rate (10 ± 1 mm/year). Overall, the results from WL, EIS, SEM, XRD, and FTIR concluded that bronopol was identified as effective biocide and corrosion inhibitor which controls the both chemical and biocorrosion of MS in CWS.
KeywordsMild steel Biofilm Cooling tower Biocorrosion Bronopol
This study was funded by University Grants Commission (MRP-MAJOR-MICRO-2013-31825). Science and Engineering Research Board, Department of Science and Technology, Government of India (EEQ/2016/000449 & SB/YS/LS-40/2013) and Department of Biotechnology, Government of India (BT/RLF/Re-entry/17/2012). J. Narenkumar acknowledge the UGC, Government of India for financial support through project scheme (UGC-MRP). Authors also thank Dr. J. Madhavan, Dr. J. Theerthagiri, and Dr. R.A. Senthil, Department of Chemistry, Thiruvalluvar University (TVU) for their help in electrochemical studies and related discussions.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest in the publication.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Bano AS, Qazi JI (2011) Soil buried mild steel corrosion by Bacillus cereus-SNB4 and its inhibition by Bacillus thuringiensis-SN8. Pak J Zool 43:555–562Google Scholar
- Beech IB, Gaylarde CC (1999) Recent advance in the study of biocorrosion—an overview. Rev Microbial 30:177–190Google Scholar
- Booth GH (ed) (1971) Microbiological corrosion, M and B monographs CE11. Mills and Boon, LondonGoogle Scholar
- Bott TR, Miller PC, Patel TD (1983) Biofouling in an industrial cooling water system. Process Biochem 10:10–20Google Scholar
- Flemming HC (1996) Economical and technical overview. In: Heitz E, Sand HC (eds) Microbially influenced corrosion of materials. Springer, HeidelbergGoogle Scholar
- Fontana MG (1986) Corrosion engineering. Mc-Graw hill, New YorkGoogle Scholar
- Frey R (1998) Award-winning biocides are lean, mean, and green. Today’s Chemist Work 7(6):34–38Google Scholar
- Harrah T, Panilaitis B, Kaplan D (2004) Microbial exopolysaccharides. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (eds) The prokaryotes. Springer, New York, pp 88–115Google Scholar
- Holt JG, Kreig NR, Sneath PHA, Stanely JT, Williams ST (eds) (1994) Bergey’s manual of determinative bacteriology. Williams & Wilkins, BaltimoreGoogle Scholar
- Hussain A, Bano AS, Qazi JI (2013) Corrosion of mild steel simulating long term soil bacteria filed conditions differing in nutritional and biotic components. World Appl Sci J 22:985–990Google Scholar
- Mcintyre PJ, Mercer AD (2010) Corrosion testing and determination of corrosion rates. In: Shreir's corrosion. Reference module in materials science and materials engineering, vol 2. Elsevier, Oxford, pp 1443–1526Google Scholar
- Okabe S, Jones WL, Lee W, Characklis WG (1994) Anaerobic SRB biofilms in industrial water systems: a process analysis. In: Geesy GG, Lewandowsky Z, Flemming HC (eds) Biofouling and biocorrosion in industrial water systems. Lewis, Boca Raton, pp 189–204Google Scholar
- Palaniappan B, Toleti SR (2015) Characterization of microfouling and corrosive bacterial community of a firewater distribution system. J Biosci Bioeng 1:7Google Scholar
- Rajasekar A, Ting YP (2014) Characterization of corrosive bacterial consortium isolated from water in a cooling tower. ISRN Corrosion 10:1155Google Scholar
- Telang AJ, Ebert S, Foght JM (1997) Effect of nitrate injection on the microbial community in an oil field as monitored by reverse sample genomeprobing. Appl Environ Microbiol 63(5):1785–1793Google Scholar
- Wagner P, Little B (1993) Impactofalloyingonmicrobiologically influenced corrosion. A review. Mater Perform 32:65–68Google Scholar
- Xu P (2012) MIC in circulating cooling water system. Water Res 4:203–206Google Scholar