3 Biotech

, 8:31 | Cite as

Precursor-directed combinatorial biosynthesis of cephalosporin analogue by endolithic actinobacterium Streptomyces sp. AL51 by utilizing thiophene derivative

  • Kaushik Bhattacharjee
  • Narasinga Rao Palepu
  • Kollipara Mohan Rao
  • Santa Ram JoshiEmail author
Original Article


Natural products or their derivatives provide a reliable resource for new drugs. The multi-step chemical reaction to produce new drug is not only expensive but also release pollutants. The precursor-based combinatorial biosynthesis (PCB) is, however, a better option to produce novel natural products with potential pharmaceutical applications. The present work is an attempt to synthesize an antibacterial compound by transforming thiophene precursor using endolithic Streptomyces sp. AL51. The Streptomyces sp. AL51 was isolated from a granite rock sample collected from Mylliem, Meghalaya, India. The isolate was identified as Streptomyces sp. based on its cultural, morphological, biochemical and molecular characteristics. The bioactive compound CAx1 was extracted from the fermentation broth. The compound was characterized by bioactivity-guided fractionation and identified by infrared, UV–visible, nuclear magnetic resonance and mass spectrometry data and identified as 7-[1-(thiophene-5-yl)-1-formamido]-3-propylenyl-3-cephem-4-carboxylic acid with molecular formula C15H14N2O4S2. The purified compound showed considerable in vitro antibacterial activity against both Gram-positive and Gram-negative bacteria showing its broad spectrum property. The obtained results provide promising baseline information for the potential use of endolithic actinobacterium for semisynthetic drug discovery. This is the first report on PCB of broad range antibacterial compound by endolithic Streptomyces strain.


Endolithic Streptomyces Cephalosporin analogue Precursor based combinatorial biosynthesis Antibacterial 



The NMR facility was provided by S.A.I.F, N.E.H.U, Shillong. The UV–Visible spectroscopic facility was provided by Central Instrumentation Facility, School of Life Sciences, N.E.H.U, Shillong and FT-IR facility was provided by C.I.F, I.A.S.S.T, Guwahati. We are also grateful to Dr. S. Sangilipandi, Centre for Advanced Studies in Chemistry, N.E.H.U, Shillong for the help during TLC and purification studies. Authors acknowledge the partial financial support by the DST-FIST programme of Department of Science & Technology, Government of India for the research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

13205_2017_1051_MOESM1_ESM.docx (588 kb)
Supplementary material 1 (DOCX 588 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Kaushik Bhattacharjee
    • 1
  • Narasinga Rao Palepu
    • 2
  • Kollipara Mohan Rao
    • 2
  • Santa Ram Joshi
    • 1
    Email author
  1. 1.Microbiology Laboratory, Department of Biotechnology & BioinformaticsNorth-Eastern Hill UniversityShillongIndia
  2. 2.Centre for Advanced Studies in ChemistryNorth-Eastern Hill UniversityShillongIndia

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