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Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi

3 Biotech volume 7, Article number: 383 (2017) Cite this article

Abstract

Statistical methodologies, including Plackett–Burman design and Box–Behnken design, were employed to optimize the fermentation conditions for the production of active substances against aquatic pathogen Vibrio harveyi by marine-derived Aspergillus flavipes strain HN4-13. The optimal crucial fermentation values for maximum production of active substances against V. harveyi were obtained as follows: X 1 (peptone) = 0.3%, X 2 (KCl) = 0.25%, and X 3 (inoculum size) = 4.5%. The predicted diameter of inhibitory zone against V. harveyi was 23.39 mm, and the practical value reached 23.71 ± 0.98 mm with a 62.3% increase. Bioassay-guided fractionation resulted in the acquisition of two compounds whose structures were identified as questin (1) and emodin (2). Questin exhibited the same antibacterial activity against V. harveyi as streptomycin (MIC 31.25 µg/mL). This is the first time to report questin as a potential antibacterial agent against aquatic pathogen V. harveyi.

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Acknowledgments

This study was funded by Natural Science Foundation of Jiangsu Province (No. BK20151283), Natural Science Foundation of Lianyungang (No. CG1612), 521 Talented Project of Lianyungang, Priority Academic Program Development of Jiangsu Higher Education Institutions (No. 5511201401X) and Open-end Funds of Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening (No. 2015HYB10).

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Affiliations

  1. Jiangsu Key Laboratory of Marine Biotechnology, College of Marine Life and Fisheries, Huaihai Institute of Technology, Lianyungang, 222005, China

    Lei Guo

  2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Institute of Marine Resources Development & Research, Lianyungang, 222004, China

    Lei Guo

  3. Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China

    Cong Wang

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  1. Lei Guo
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  2. Cong Wang
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Correspondence to Lei Guo.

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Guo, L., Wang, C. Optimized production and isolation of antibacterial agent from marine Aspergillus flavipes against Vibrio harveyi . 3 Biotech 7, 383 (2017). https://doi.org/10.1007/s13205-017-1015-z

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  • DOI: https://doi.org/10.1007/s13205-017-1015-z

Keywords

  • Aspergillus flavipes
  • Vibrio harveyi
  • Fermentation optimization
  • Response surface methodology
  • Bioassay-guided fractionation
  • Questin