Abstract
Enzymatic hydrolysis of seaweed biomass was studied using xylanase produced from marine bacteria Bacillus sp. strain BT21 through solid-state fermentation of wheat bran. Three types of seaweeds, Ahnfeltia plicata, Padina tetrastromatica and Ulva lactuca, were selected as representatives of red, brown, and green seaweeds, respectively. Seaweed biomass was pretreated with hot water. The efficiency of pretreated biomass to release reducing sugar by the action of xylanase as well as the type of monosaccharide released during enzyme saccharification of seaweed biomass was studied. It was seen that pretreated biomass of seaweed A. plicata, U. lactuca, and P. tetrastroma, at 121 °C for 45 min, followed by incubation with 50 IU xylanase released reducing sugars of 233 ± 5.3, 100 ± 6.1 and 73.3 ± 4.1 µg/mg of seaweed biomass, respectively. Gas chromatography analysis illustrated the release of xylose, glucose, and mannose during the treatment process. Hot water pre-treatment process enhanced enzymatic conversion of biomass into sugars. This study revealed the important role of xylanase in saccharification of seaweed, a promising feedstock for third-generation bioethanol production.
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Acknowledgements
The authors are grateful to the Director, National Institute of Oceanography (CSIR), Goa (India) for providing necessary facilities and Dr. N. Ramaiah for encouragement and support. Authors thank Ram Murti Meena for gene sequencing. Authors thank DST, New Delhi and CSIR-funded project PSC0206 for financial support. This is National Institute of Oceanography (NIO) contribution number 8328.
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Parab, P., Khandeparker, R., Amberkar, U. et al. Enzymatic saccharification of seaweeds into fermentable sugars by xylanase from marine Bacillus sp. strain BT21. 3 Biotech 7, 296 (2017). https://doi.org/10.1007/s13205-017-0921-4
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DOI: https://doi.org/10.1007/s13205-017-0921-4