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High frequency direct shoot organogenesis of leaf explants and a comparative evaluation of phytochemicals, antioxidant potential of wild vs. in vitro plant extracts of Lysimachia laxa

3 Biotech volume 7, Article number: 274 (2017) Cite this article

Abstract

The present studies were attempted to develop direct shoot organogenesis from in vitro grown leaf explants of Lysimachia laxa and comparative evaluation of phytochemical and antioxidant potential of in vitro raised and wild plants extracts. The fresh leaves of this species are used for deworming gastrointestinal worm infection in traditional medicine. Overexploitation of this species and poor regeneration has led to rapid decline in wild population, therefore, present investigation was attempted to develop an efficient rapid mass propagation protocol for this species. Our result showed significantly (P < 0.05) high adventitious shoot proliferation of 17.21 ± 0.24 number per leaf explants cultured in Murashige and Skoog medium fortified with 1.25 mg L−1 thiadizuron and 1.0 mg L−1 α-naphthalene acetic acid. Further enhancement was achieved through elongation medium fortified with 1.0 mg L−1 6-benzylaminopurine by average shoot number of 31.1 ± 0.80 and length of 5.96 ± 0.13 cm. Murashige and Skoog medium fortified with 0.50 mg L−1 Indole-3-acetic acid showed high rooting induction (100%) with average root number of 11.70 and length 7.35 cm. All rooted plants were successfully acclimatized in greenhouse and transferred to field condition with a survival rate of 97%. The contents of phenolic and flavonoid were higher in in vitro raised plant in compared to wild plant extracts. Antioxidants assay showed high radical scavenging activity of IC50 1.61 ± 0.07 mg dry material and reducing power of 49.79 ± 0.11 mg/g ascorbic acid equivalent by aqueous methanol extracts of in vitro raised 3-months-old plants in compare to the wild plants. The present protocol is a viable option for pharmaceutical or nutraceutical industries for sustainable utilization of L. laxa with enhanced of phytochemical and antioxidant potency which is not reported elsewhere.

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Acknowledgements

All authors acknowledge the Head of Office, BSI Shillong for providing necessary facilities and Director, BSI, Kolkata for giving financial assistance throughout the research work.

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Author notes

  1. Sanjoy Gupta

    Present address: Andaman and Nicobar Centre for Ocean Science and Technology (ANCOST), National Institute of Ocean Technology (NIOT), Port Blair, AN Islands, 744103, India

Affiliations

  1. Botanical Survey of India, Eastern Regional Center, Shillong, Meghalaya, 793003, India

    Sanjoy Gupta & A. A. Mao

  2. Plant Chemistry Department, Botanical Survey of India, Howrah, India

    Tapan Seal

  3. Department of Biotechnology, Gauhati University, Guwahati, Assam, 781014, India

    Soneswar Sarma

Authors
  1. Sanjoy Gupta
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  2. Tapan Seal
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Contributions

SG implemented the all experiments, analyzed the data and wrote the manuscript. AAM, TS and SS planned the work, edited the manuscript and monitored the entire research work.

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Correspondence to Sanjoy Gupta.

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All authors declare that they have no any conflict of interest in this publication. This publication is approved by the author and all coauthors. The work was carried out at Plant Tissue culture Lab, Botanical Survey of India, ERC, Shillong.

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Gupta, S., Seal, T., Mao, A.A. et al. High frequency direct shoot organogenesis of leaf explants and a comparative evaluation of phytochemicals, antioxidant potential of wild vs. in vitro plant extracts of Lysimachia laxa . 3 Biotech 7, 274 (2017). https://doi.org/10.1007/s13205-017-0907-2

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  • DOI: https://doi.org/10.1007/s13205-017-0907-2

Keywords

  • Lysimachia laxa
  • Direct organogenesis
  • Phytochemicals
  • Antioxidant
  • In vitro and wild plant extract