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Prevalence of TEM, SHV, and CTX-M genes of extended-spectrum β-lactamase-producing Escherichia coli strains isolated from urinary tract infections in adults

3 Biotech volume 7, Article number: 244 (2017) Cite this article

Abstract

Urinary tract infections (UTIs) are one of the major sources of widespread infectious diseases in the community as well as in the hospitals which increase the cause of morbidity and mortality. Prevalence of extended-spectrum-β-lactamase (ESBL)-producing uropathogenic E. coli isolates has been found to be increased rapidly across the world. The present study was undertaken to find out the frequency of bla TEM, bla CTX-M, and bla SHV genes among E. coli isolates from UTI and detect their sensitivity pattern. A total of 112 non-repeated E. coli isolates obtained from urine samples of UTI diagnosed patients were included in this study. Antibiotic susceptibility test was done by disc diffusion method. Seventy seven (68.75%) isolates were MDR and tested for ESBL. ESBL-positive isolates were screened for bla TEM, bla CTX-M, and bla SHV genes by monoplex PCR (polymerase chain reaction). Among 46 ESBL-producing E. coli isolates, 8.69% harboured all the three bla genes. The bla TEM was the predominant (93.47%) gene followed by bla CTX-M (82.6%) and bla SHV (4.34%). It can be concluded that the prevalence of MDR (multidrug resistance) ESBL-producing E. coli appears to be high and the highest identified gene was bla TEM. The knowledge of resistance pattern can help physician’s select suitable empirical antibiotic regimens, so that antibiotics showing high-resistance pattern can be avoided.

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Acknowledgements

We are grateful to Prof. Dr. DK Roy, Dean, IMS and Sum Hospital (S‘O’A University) for extended essential laboratory facilities. Our sincere thanks are due to Prof. Dr. M.R. Nayak, Honourable President, Siksha ‘O’ Anusandhan University, Bhubaneswar, for providing financial support.

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Affiliations

  1. Department of Microbiology, IMS and SUM Hospital, Siksha ‘O’ Anusandhan University, Bhubaneswar, Odisha, India

    Jayanti Jena & Nagen Kumar Debata

  2. Centre for Biotechnology, Siksha ‘O’ Anusandhan University, Khandagiri, Bhubaneswar, Odisha, 751003, India

    Rajesh Kumar Sahoo & Enketeswara Subudhi

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  1. Jayanti Jena
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  2. Rajesh Kumar Sahoo
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  3. Nagen Kumar Debata
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  4. Enketeswara Subudhi
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Correspondence to Enketeswara Subudhi.

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Jena, J., Sahoo, R.K., Debata, N.K. et al. Prevalence of TEM, SHV, and CTX-M genes of extended-spectrum β-lactamase-producing Escherichia coli strains isolated from urinary tract infections in adults. 3 Biotech 7, 244 (2017). https://doi.org/10.1007/s13205-017-0879-2

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  • DOI: https://doi.org/10.1007/s13205-017-0879-2

Keywords

  • Urinary tract infection
  • Escherichia coli
  • bla TEM
  • bla SHV
  • bla CTX-M