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Xanthan gum production using jackfruit-seed-powder-based medium: optimization and characterization

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Abstract

Xanthan gum (XG) production by Xanthomonas campestris NCIM 2961 using jackfruit seed powder (JSP) as a novel substrate was reported. Central composite design (CCD) of response surface method (RSM) was used to evaluate the linear and interaction effects of five medium variables (JSP, peptone, citric acid, K2HPO4 and KH2PO4) for XG production. Maximum XG production (51.62 g/L) was observed at the optimum level of JSP (4 g/L), peptone (0.93 g/L), citric acid (0.26 g/L), K2HPO4 (1.29 g/L) and KH2PO4 (0.5 g/L). K2HPO4 and KH2PO4 were found as significant medium components, which served as buffering agents as well as nutrients for X. campestris growth. The obtained biopolymer was characterized as XG by XRD and FTIR analysis. Results of this study revealed that JSP was found to be a suitable low cost substrate for XG production.

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Acknowledgements

The authors greatly acknowledge the Management, Director, and the Head, Department of Biotechnology, SRM University, Kattankulathur, for their encouragement and providing of facilities to carry out this study.

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Authors and Affiliations

  1. Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, 603 203, India

    R. Felicia Katherine & R. Jaiganesh

  2. Department of Industrial Biotechnology, Government College of Technology, Coimbatore, 641 013, India

    C. Muthukumaran & G. Sharmila

  3. Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai, 603 203, India

    N. Manoj Kumar

  4. Department of Chemical Engineering, School of Bioengineering, SRM University, Kattankulathur, Chennai, 603 203, India

    K. Tamilarasan

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  1. R. Felicia Katherine
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  2. C. Muthukumaran
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  3. G. Sharmila
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  4. N. Manoj Kumar
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Correspondence to C. Muthukumaran.

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Felicia Katherine, R., Muthukumaran, C., Sharmila, G. et al. Xanthan gum production using jackfruit-seed-powder-based medium: optimization and characterization. 3 Biotech 7, 248 (2017). https://doi.org/10.1007/s13205-017-0876-5

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  • DOI: https://doi.org/10.1007/s13205-017-0876-5

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