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Evaluation of fermentation kinetics of acid-treated corn cob hydrolysate for xylose fermentation in the presence of acetic acid by Pichia stipitis

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Abstract

The efficient utilization of lignocellulosic biomass for ethanol production depends on the fermentability of the biomass hydrolysate obtained after pretreatment. In this work we evaluated the kinetics of ethanol production from xylose using Pichia stipitis in acid-treated corn cob hydrolysate. Acetic acid is one of the main inhibitors in corn cob hydrolysate that negatively impacts kinetics of xylose fermentation by P. stipitis. Unstructured kinetic model has been formulated that describes cell mass growth and ethanol production as a function of xylose, oxygen, ethanol, and acetic acid concentration. Kinetic parameters were estimated under different operating conditions affecting xylose fermentation. This is the first report on kinetics of xylose fermentation by P. stipitis which includes inhibition of acetic acid on growth and product formation. In the presence of acetic acid in the hydrolysate, the model accurately predicted reduction in maximum specific growth rate (from 0.23 to 0.15 h−1) and increase in ethanol yield per unit biomass (from 3 to 6.2 gg−1), which was also observed during experimental trials. Presence of acetic acid in the fermentation led to significant reduction in the cell growth rate, reduction in xylose consumption and ethanol production rate. The developed model accurately described physiological state of P. stipitis during corn cob hydrolysate fermentation. Proposed model can be used to predict the influence of xylose, ethanol, oxygen, and acetic acid concentration on cell growth and ethanol productivity in industrial fermentation.

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Abbreviations

dp′:

Average of experimental values

dp:

Experimental value

Xp:

Value predicted by mathematical model

np:

Number of experiment points

μ :

Specific growth rate (h−1)

q s :

Specific substrate uptake rate (h−1)

q p :

Specific ethanol production rate (h−1)

C ox :

Dissolved oxygen concentration (mg L−1)

X :

Cell mass concentration (g L−1)

μ max :

Maximum specific growth rate (h−1)

S :

Xylose concentration (g L−1)

K S :

Saturation constant governing xylose-limited growth (g L−1)

K I :

Substrate inhibition constant for growth (g L−1)

P :

Ethanol concentration (g L−1)

n :

Exponents governing ethanol inhibition of growth

P max :

Maximum ethanol concentration allowing growth (g L−1)

K ox :

Saturation constant for oxygen-limited growth (mg L−1)

C A :

Acetic acid concentration (g L−1)

C Amax :

Maximum concentration of acetic acid at which cell growth ceases (g L−1)

m :

Parameter used to describe acetic acid inhibition

Y x/s :

Biomass yield (g g−1)

m S :

Maintenance coefficient (g g−1 h−1)

Y p/x :

Yield of ethanol per biomass formed (g g−1)

K La :

Volumetric mass transfer coefficient (s−1)

C ox*:

Critical dissolved oxygen concentration (mg L−1)

Y x/ox :

Yield of biomass per oxygen consumed (g mg−1)

h :

Time (h)

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Acknowledgements

The authors gratefully acknowledge Praj industries Ltd. Pune for financial support to conduct the research work. The authors are also thankful to analytical team of Praj Matrix—R&D Center for providing assistance with analytical measurements.

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Authors and Affiliations

  1. Department of Technology, Savitribai Phule Pune University, Ganeshkhind, Pune, Maharashtra, 411007, India

    Mohan Kashid & Anand Ghosalkar

  2. Praj Matrix R&D Center, Division of Praj Industries Ltd., Urawade, Pirangut, Pune, 412115, India

    Mohan Kashid & Anand Ghosalkar

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  1. Mohan Kashid
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Correspondence to Mohan Kashid.

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Kashid, M., Ghosalkar, A. Evaluation of fermentation kinetics of acid-treated corn cob hydrolysate for xylose fermentation in the presence of acetic acid by Pichia stipitis . 3 Biotech 7, 240 (2017). https://doi.org/10.1007/s13205-017-0873-8

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