Abstract
A total of 148 accessions representing six important species of the genus Stylosanthes, including S. guianensis, S. hamata, S. scabra, S. seabrana, S. macrocephala, and S. capitata, were used to evaluate genetic variation and relationships using sequence-related amplified polymorphism markers. The results showed that the 18 selected primer pairs generated 138 distinct fragments. The fragment sizes ranged from 150 to 2000 bp. Genetic similarity coefficients among the 148 accessions ranged from 0.51 to 0.99, with an average of 0.79. The effective allele number (ne) generated by the 18 primer pairs averaged 1.3552 and ranged from 1.2069 to 1.6080; Nei’s gene diversity (He) ranged from 0.1304 to 0.3207, with an average of 0.2070; and Shannon’s information index (I) averaged 0.3213 and ranged from 0.2233 to 0.4582. The unweighted pair-group method with arithmetic averages at the 0.69 similarity level separated the 148 accessions into two distinct groups. One group belonged to S. guianensis, and the other group belonged to the non-S. guianensis type. This study verified that Stylosanthes have rich genetic variation, which is an excellent basis for Stylosanthes breeding for new cultivars. This study demonstrates that the SRAP technique is a reliable tool for differentiating Stylosanthes accessions and for discerning genetic relationship among them.
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Acknowledgements
The authors would like to thank the editors and the anonymous reviewers for their valuable comments to improve this manuscript. This research was supported by the National Natural Science Foundation of China (No. 31402124) and the earmarked fund for Modern Agro-industry Technology Research System (CARS-35).
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CH and GL conceived and designed the experiments; CH performed the experiment, analyzed the data, and wrote this manuscript; GL and CB were responsible for the accessions collection, and discussing and reviewing the manuscript. All authors had read, critically edited, and approved the final manuscript.
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Huang, C., Liu, G. & Bai, C. Polymorphism analysis in identification of genetic variation and relationships among Stylosanthes species. 3 Biotech 7, 39 (2017). https://doi.org/10.1007/s13205-017-0705-x
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DOI: https://doi.org/10.1007/s13205-017-0705-x