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EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars

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Abstract

Turmeric (Curcuma longa L., family Zingiberaceae) is one of the most economically important plants for its use in food, medicine, and cosmetic industries. Cultivar identification is a major constraint in turmeric, owing to high degree of morphological similarity that in turn, affects its commercialization. The present study addresses this constraint, using EST-SSR marker based, molecular identification of 8 elite cultivars and 88 accessions in turmeric. Fifty EST-SSR primers were screened against eight cultivars of turmeric (Suroma, Roma, Lakadong, Megha, Alleppey Supreme, Kedaram, Pratibha, and Suvarna); out of which 11 primers showed polymorphic banding pattern. The polymorphic information content (PIC) of these primers ranged from 0.13 to 0.48. However, only three SSR loci (CSSR 14, CSSR 15, and CSSR 18) gave reproducible unique banding pattern clearly distinguishing the cultivars ‘Lakadong’ and ‘Suvarna’ from other cultivars tested. These three unique SSR markers also proved to be effective in identification of ‘Lakadong’ cultivars when analysed with 88 accessions of turmeric collected from different agro-climatic regions. Furthermore, two identified cultivars (Lakadong and Suvarna) could also be precisely differentiated when analysed and based on phylogenetic tree, with other 94 genotypes of turmeric. The novel SSR markers can be used for identification and authentication of two commercially important turmeric cultivars ‘Lakadong’ and ‘Suvarna’.

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References

  • Anandaraj M, Prasath D, Kandiannan K, Zachariah TJ, Srinivasan V, Jha AK, Singh BK, Singh AK, Pandey VP, Singh SP, Shoba N (2014) Genotype by environment interaction effects on yield and curcumin in turmeric (Curcuma longa L.). Ind Crop Prod 53:358–364

    Article  CAS  Google Scholar 

  • ASTA (1958) Official analytical methods. American Spice Trade Association: Englewood Cliffs, NJ, Method 18.0 (Color power of turmeric)

  • Basheer-Salimia R, Lorenzi S, Batarseh F, Moreno-Sanz P, Emanuelli F, Grando MS (2014) Molecular identification and genetic relationships of Palestinian grapevine cultivars. Mol Biotech. 56:546–556

    Article  CAS  Google Scholar 

  • Behera L, Patra BC, Sahu RK, Nanda A, Sahu SC, Patnaik A, Rao GJ, Singh ON (2012) Assessment of genetic diversity in medicinal rices using microsatellite markers. Aust J Crop Sci 6:1369

    Google Scholar 

  • Chen L, Ma Q, Chen Y, Wang B, Pei D (2014) Identification of major walnut cultivars grown in China based on nut phenotypes and SSR markers. Sci Hort. 26:168–240

    Article  Google Scholar 

  • Chrobokova E, Raddová J, Vachůn M, Krška B, Pidra M (2011) An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers. Hort Sci (Prague). 38:125–133

    CAS  Google Scholar 

  • Corcolon EA, Laurena AC, Dionisio-Sese ML (2015) Genotypic characterization of turmeric (Curcuma longa L.) accessions from Mindanao, Philippines using RAPD markers. Procedia Chem 14:157–163

    Article  CAS  Google Scholar 

  • Doyl JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Hong JH, Kwon YS, Mishra RK, Kim DH (2015) Construction of EST-SSR databases for effective cultivar identification and their applicability to complement for Lettuce (Lactuca sativa L.) distinctness test. Am J Plant Sci 6:113

    Article  Google Scholar 

  • Jan HU, Rabbani MA, Shinwari ZK (2011) Assessment of genetic diversity of indigenous turmeric (Curcuma longa L.) germplasm from Pakistan using RAPD markers. J Med Plant Res 4:823–830

    Google Scholar 

  • Joshi RK, Kuanar A, Mohanty S, Subudhi E, Nayak S (2010) In silico mining and characterization of EST derived microsatellites from Curcuma longa L. Bioinformation 5:128–131

    Article  Google Scholar 

  • Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK (2011) Microsatellite markers: an overview of the recent progress in plants. Euphytica 177:309–334

    Article  CAS  Google Scholar 

  • Khan H, Sivalingam PN, Chauhan S, Awasthi OP, More TA (2013) Improved crossing technique and identification of true F 1 hybrids of Ziziphus mauritiana Lam. by molecular markers. Sci Hort 150:164–171

    Article  Google Scholar 

  • Koussao S, Gracen V, Asante I, Danquah EY, Ouedraogo JT, Baptiste TJ, Vianney TM (2014) Diversity analysis of sweet potato (Ipomoea batatas [L.] Lam) germplasm from Burkina Faso using morphological and simple sequence repeats markers. Afr J Biotechnol 13:729–742

    Article  CAS  Google Scholar 

  • Leong-Skorhickova J, Sida O, Jarolimova V, Sabu M, Fer T, Travnicek P, Suda J (2007) Chromosome numbers and genome size variation in Indian species of Curcuma (Zingiberaceae). Ann Bot 100:505–526

    Article  Google Scholar 

  • Liu GS, Zhang YG, Tao R, Fang JG, Dai HY (2014) Identification of apple cultivars on the basis of simple sequence repeat markers. Genet Mol Res 13:7377

    Article  CAS  Google Scholar 

  • Madhou M, Normand F, Bahorun T, Hormaza JI (2013) Fingerprinting and analysis of genetic diversity of litchi (Litchi chinensis Sonn.) accessions from different germplasm collections using microsatellite markers. Tree Genet Genomes 9:387–396

    Article  Google Scholar 

  • Nair RR, Shiva KN, Anchu S, Zachariah TJ (2010) Characterization of open-pollinated seedling progenies of turmeric (Curcuma longa L.) based on chromosome number, plant morphology, rhizome yield and rhizome quality. Cytologia 75:443–449

    Article  Google Scholar 

  • Nayak S, Naik PK, Acharya LK, Pattnaik AK (2006) Detection and evaluation of genetic variation in 17 promising cultivars of turmeric (Curcuma longa L.) using 4C nuclear DNA content and RAPD markers. Cytologia 71:49–55

    Article  CAS  Google Scholar 

  • Nicot N, Chiquet V, Gandon B, Amilhat L, Legeai F, Leroy P, Bernard M, Sourdille P (2004) Study of simple sequence repeat (SSR) markers from wheat expressed sequence tags (ESTs). Theor Appl Genet 109:800–805

    Article  CAS  Google Scholar 

  • Perrier X, Jacquemoud-Collet JP (2013) Darwin software. http://darwin.cirad.fr/

  • Rama Rao M, Rao DV (1994) Genetic resources of turmeric, advances in horticulture. Plantation and spice crops. Malhotra Publishing House, New Delhi

    Google Scholar 

  • Ravindran PN, Babu KN, Sivaraman K (2007) (eds) Turmeric: the genus Curcuma. CRC Press

  • Rocha EA, Paiva LV, Carvalho HH, Guimarães CT (2010) Molecular characterization and genetic diversity of potato cultivars using SSR and RAPD markers. Crop Breed Appl Biotechnol 10:204–210

    Article  CAS  Google Scholar 

  • Saal B, Wricke G (1999) Development of simple sequence repeat markers in rye (Secale cereale L.). Genome 42:964–972

    Article  CAS  Google Scholar 

  • Sameh MR, Olfa SD, Saleh B, Ali F (2014) Microsatellite marker-based identification and genetic relationships of millennium olive cultivars in Tunisia. Int J Agro Agric Res 5:55–63

    Google Scholar 

  • Sasikumar B (2005) Genetic resources of Curcuma: diversity, characterization and utilization. Plant Genet Resour 3:230–251

    Article  CAS  Google Scholar 

  • Scott KD, Eggler P, Seaton G, Rossetto M, Ablett EM, Lee LS, Henry RJ (2000) Analysis of SSRs derived from grape ESTs. Theor Appl Genet 100:723–726

    Article  CAS  Google Scholar 

  • Sehgal SA, Tahir RA, Nawaz M (2012) Molecular characterization of wheat genotypes using SSR markers. Int J Bioautomation 16:119–128

    CAS  Google Scholar 

  • Selvan MT, Thomas KG, Manojkumar K (2002) Ginger (Zingiber officinale Rosc.). Indian spices-production and utilization. Coconut Development Board, India, pp 110–131

    Google Scholar 

  • Senan S, Kizhakayil D, Sheeja TE, Sasikumar B, Bhat AI, Parthasarathy VA (2013) Novel polymorphic microsatellite markers from turmeric, Curcuma longa L. (Zingiberaceae). Acta Bot Croat 72:407–412

    CAS  Google Scholar 

  • Shamina A, Zachariah TJ, Sasikumar B, George JK (1998) Biochemical variation in turmeric (Curcuma longa Linn.) accessions based on isozyme polymorphism. J Hortic Sci Biotechnol 73:479–483

    Article  CAS  Google Scholar 

  • Siju S, Dhanya K, Syamkumar S, Sasikumar B, Sheeja TE, Bhat AI, Parthasarathy VA (2010) Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.). Mol Biotechnol 44(2):140–147

    Article  CAS  Google Scholar 

  • Singh S, Joshi RK, Nayak S (2013) Identification of elite genotypes of turmeric through agro climatic zone based evaluation of important drug yielding traits. Ind Crop Prod 43:165–171

    Article  CAS  Google Scholar 

  • Singh AK, Nanda P, Singh A, Singh B (2015) Genetic diversity analysis in turmeric (Curcuma Longa L.) based on SSR markers. JBERR 2(1):20–24

    Google Scholar 

  • Syamkumar S (2008) Molecular Biochemical and morphological characterization of selected Curcuma accessions. Thesis, Calicut:Calicut University

  • Trujillo I, Ojeda MA, Urdiroz NM, Potter D, Barranco D, Rallo L, Diez CM (2014) Identification of the Worldwide Olive Germplasm Bank of Córdoba (Spain) using SSR and morphological markers. Tree Genet Genomes 10:141–155

    Article  Google Scholar 

  • UPOV/INF/17/1 (2010) Guideline for DNA-profiling: molecular marker selection and database construction “BMT Guideline”. Geneva

  • Varshney RK, Graner A, Sorrells ME (2005) Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23:48–55

    Article  CAS  Google Scholar 

  • Verma S, Singh S, Sharma S, Tewari SK, Roy RK, Goel AK, Rana TS (2015) Assessment of genetic diversity in indigenous turmeric (Curcuma longa) germplasm from India using molecular markers. Physiol Mol Biol Plants 21:233–242

    Article  CAS  Google Scholar 

  • Wang JY, Chuang KC (2013) Development of novel microsatellite markers for effective applications in Anthurium cultivar identification. Euphytica 189:421–431

    Article  CAS  Google Scholar 

  • Xie RJ, Zhou J, Wang GY, Zhang SM, Chen L, Gao ZS (2011) Cultivar identification and genetic diversity of Chinese bayberry (Myrica rubra) accessions based on fluorescent SSR markers. Plant Mol Biol Report 29:554–562

    Article  Google Scholar 

Download references

Acknowledgements

We are thankful to our President Prof. Manoj Ranjan Nayak and Dean and Prof. S. C. Si for constant support and encouragement in and out of the centre. Financial assistance from DST, Govt. of India, New Delhi is duly acknowledged. We are also thankful to Dr. Sasikumar of IISR, Kerla, Dr. S. K. Barik of NEHU, Meghalaya, and Dr. Parshuram Sial of HARS, Patangi, Koraput for providing registered varieties of turmeric.

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  1. Centre for Biotechnology, Siksha O Anusandhan University, Bhubaneswar, Odisha, 751003, India

    Ambika Sahoo, Sudipta Jena, Basudeba Kar, Suprava Sahoo, Asit Ray, Subhashree Singh, Raj Kumar Joshi, Laxmikanta Acharya & Sanghamitra Nayak

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  1. Ambika Sahoo
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  2. Sudipta Jena
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Correspondence to Basudeba Kar or Sanghamitra Nayak.

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Sahoo, A., Jena, S., Kar, B. et al. EST-SSR marker revealed effective over biochemical and morphological scepticism towards identification of specific turmeric (Curcuma longa L.) cultivars. 3 Biotech 7, 84 (2017). https://doi.org/10.1007/s13205-017-0701-1

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