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Biodegradation of ciprofloxacin by white rot fungus Pleurotus ostreatus

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Abstract

Unrestricted and reckless use of antibiotics has resulted in their accumulation in environment. This, in turn, has led to the emergence of multiple drug-resistant microbes. The present study focuses on degradation of ciprofloxacin (CIP) by an edible white rot fungus Pleurotus ostreatus. Effect of CIP was determined on radial growth and biomass of P. ostreatus. Titrimetric and spectrophotometric assays were carried out to assess the degrading potential of P. ostreatus towards CIP. It was found that CIP has a stimulatory effect on growth and enzyme activity of P. ostreatus. Maximum enzyme (glucanase, ligninases, laccase) production was observed at the highest concentration of CIP (500 ppm). Antibiotic degradation of about 68.8, 94.25 and 91.34% was estimated after 14 days of incubation at 500 ppm CIP using Titrimetric, Indigo carmine and Methyl orange assay, respectively. Degradation of CIP was further validated by high performance liquid chromatography (HPLC) and microbiological analysis. HPLC analysis revealed 95.07% degradation while microbiological test also exhibited a decreased antimicrobial activity of degraded products against Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes. To the best of our knowledge, this is the first study wherein P. ostreatus was used for the degradation of ciprofloxacin.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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  1. School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 1440111, India

    Sushil Kumar Singh, Robinka Khajuria & Loveleen Kaur

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  1. Sushil Kumar Singh
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  2. Robinka Khajuria
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  3. Loveleen Kaur
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Correspondence to Loveleen Kaur.

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Singh, S.K., Khajuria, R. & Kaur, L. Biodegradation of ciprofloxacin by white rot fungus Pleurotus ostreatus . 3 Biotech 7, 69 (2017). https://doi.org/10.1007/s13205-017-0684-y

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