Skip to main content

Advertisement

SpringerLink
Log in

Green synthesis of silver oxide nanoparticles and its antibacterial activity against dental pathogens

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

In the present study, the use of silver oxide nanoparticles (Ag2O NPs) synthesized using Ficus benghalensis prop root extract (FBPRE) as a reducing and stabilizing agent is reported and evaluated for its antibacterial activity against dental bacterial strains. The effects of pH, extract concentration, metal ion concentration, and contact time were studied to confirm the optimum production of Ag2O NPs. Our results suggest that, by increasing the extract concentration and the time frame, there will be a significant increase in the formation of nanoparticles. The UV–vis adsorption spectra show the absorbance peak in the range of 430 nm, and FTIR spectral peaks indicate that the phytochemicals in the extract are responsible for the formation of the nanoparticles. The HR-TEM image, SAED, and XRD pattern confirmed the morphology (spherical), silver oxide 42.7 nm and silver 51.4 nm, and crystalline nature of the obtained nanoparticles, respectively. The blend of FBPRE and Ag2O NPs showed excellent antibacterial activities against the two-dental bacteria Streptococcus mutans and Lactobacilli sp. The study results suggest that the blend of synthesized Ag2O NPS and FBPRE will be useful in tooth paste as a germicidal agent after extensive investigation with animal models.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmad S, Rao H, Akhtar M, Ahmad I, Hayat MM, Iqbal Z (2011) Phytochemical composition and pharmacological prospectus of Ficus bengalensis Linn. (Moraceae)-A review. J Med Plants Res 5:6393–6400

    CAS  Google Scholar 

  • Ahmed S, Ahmad M, Swami BL, Ikram S (2016) Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci 9:1–7

    Article  Google Scholar 

  • Amin M, Anwar F, Janjua MR, Iqbal MA, Rashid U (2012) Green synthesis of silver nanoparticles through reduction with Solanum xanthocarpum L. berry extract: characterization, antimicrobial and urease inhibitory activities against Helicobacter pylori. Int J Mol Sci 13:9923–9941

    Article  CAS  Google Scholar 

  • Aswar M, Aswar U, Watkar B, Vyas M, Wagh A, Gujar K (2008) Anthelmintic activity of Ficus benghalensis. Int J Green Pharm 2:170–172

    Article  Google Scholar 

  • Chitra K, Annadurai G (2014) Antibacterial activity of pH-dependent biosynthesized silver nanoparticles against clinical pathogen. Biomed Res Int. doi:10.1155/2014/725165

    Google Scholar 

  • Dong C, Cao C, Zhang X, Zhan Y, Wang X, Yang X, Zhou K, Xiao X, Yuan B (2017) Wolfberry fruit (Lycium barbarum) extract mediated novel route for the green synthesis of silver nanoparticles. Optik 130:162–170

    Article  CAS  Google Scholar 

  • Dwivedi AD, Gopal K (2010) Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloids Surf A 369:27–33

    Article  CAS  Google Scholar 

  • Ibrahim HM (2015) Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. J Radiat Res Appl Sci 8:265–275

    Article  Google Scholar 

  • Kora AJ, Sashidhar RB, Arunachalam J (2010) Gum kondagogu (Cochlospermum gossypium): a template for the green synthesis and stabilization of silver nanoparticles with antibacterial application. Carbohyd Polym 82:670–679

    Article  CAS  Google Scholar 

  • Kumar G, Karthik L, Bhaskara Rao KV (2010) Antimicrobial activity of latex of Calotropis gigantea against pathogenic microorganisms-an in vitro study. Pharmacologyonline 3:155–163

    Google Scholar 

  • Lee HJ, Song JY, Kim BS (2013) Biological synthesis of copper nanoparticles using Magnolia kobus leaf extract and their antibacterial activity. J Chem Technol Biotechnol 88:1971–1977

    CAS  Google Scholar 

  • Loesche WJ (1986) Role of Streptococcus mutans in human dental decay. Microbiol Rev 50:353–380

    CAS  Google Scholar 

  • Marcotte H, Lavoie MC (1998) Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 62:71–109

    CAS  Google Scholar 

  • Marsh PD (2010) Microbiology of dental plaque biofilms and their role in oral health and caries. Dent Clin North Am 54:441–454

    Article  Google Scholar 

  • Muthukrishnan S, Bhakya S, Kumar TS, Rao MV (2015) Biosynthesis, characterization and antibacterial effect of plant-mediated silver nanoparticles using Ceropegia thwaitesii–an endemic species. Ind Crops Prod 63:119–124

    Article  CAS  Google Scholar 

  • Nidhiya IS, Pai KS, Rao MC (2010) Free radical scavenging activity of extracts of young prop roots of ficus bengalensis. Pharmacologyonline 2:299–307

    Google Scholar 

  • Okafor F, Janen A, Kukhtareva T, Edwards V, Curley M (2013) Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. Int J Environ Res Publ Health 10:5221–5238

    Article  Google Scholar 

  • Palanisamy S, Rajasekar P, Vijayaprasath G, Ravi G, Manikandan R, Prabhu NM (2017) A green route to synthesis silver nanoparticles using Sargassum polycystum and its antioxidant and cytotoxic effects: an in vitro analysis. Mater Lett 189:196–200

    Article  CAS  Google Scholar 

  • Panday DR, Rauniar GP (2016) Effect of root-extracts of Ficus benghalensis (Banyan) in memory, anxiety, muscle co-ordination and seizure in animal models. BMC Complement Altern Med 16:429–441

    Article  Google Scholar 

  • Pourmortazavi SM, Taghdiri M, Makari V, Rahimi-Nasrabadi M (2015) Procedure optimization for green synthesis of silver nanoparticles by aqueous extract of Eucalyptus oleosa. Spectrochim Acta Mol Biomol Spectrosc 136:1249–1254

    Article  CAS  Google Scholar 

  • Raut Rajesh W, Lakkakula Jaya R, Kolekar Niranjan S, Mendhulkar Vijay D, Kashid Sahebrao B (2009) Phytosynthesis of silver nanoparticle using Gliricidia sepium (Jacq.). Curr Nanosci 5:117–122

    Article  Google Scholar 

  • Shankar SS, Rai A, Ahmad A, Sastry M (2004) Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 275:496–502

    Article  CAS  Google Scholar 

  • Vanaja M, Rajeshkumar S, Paulkumar K, Gnanajobitha G, Malarkodi C, Annadurai G (2013) Phytosynthesis and characterization of silver nanoparticles using stem extract of Coleus aromaticus. Int J Mater Biomater Appl 3:1–4

    Google Scholar 

  • Velmurugan P, Lee SM, Cho M, Park JH, Seo SK, Myung H, Bang KS, Oh BT (2014) Antibacterial activity of silver nanoparticle-coated fabric and leather against odor and skin infection causing bacteria. Appl Microbiol Biotechnol 98:8179–8189

    Article  CAS  Google Scholar 

  • Velmurugan P, Sivakumar S, Song YC, Jang SH (2015) Crystallization of silver metal by extract of Prunus × yedoensis Matsumura blossoms and its potential characterization. J Ind Eng Chem 31:39–42

    Article  CAS  Google Scholar 

  • Velmurugan P, Shim J, Bang KS, Oh BT (2016) Gold nanoparticles mediated coloring of fabrics and leather for antibacterial activity. J Photochem Photobiol B 160:102–109

    Article  CAS  Google Scholar 

  • Verma A, Mehata MS (2016) Controllable synthesis of silver nanoparticles using Neem leaves and their antimicrobial activity. J Radiat Res Appl Sci 9:109–115

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the BK21 plus program through the National Research Foundation (NRF) funded by the Ministry of Education of Korea. This work was also supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2016R1C1B2009865).

Author information

Authors and Affiliations

  1. Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 54596, South Korea

    Velu Manikandan, Palanivel Velmurugan, Jung-Hee Park, Min Cho & Byung-Taek Oh

  2. Department of Environmental Science, Periyar University, Salem, 636011, Tamil Nadu, India

    Velu Manikandan & Palaniyappan Jayanthi

  3. Department of Biology, University of Texas-Arlington, Arlington, TX, 76019, USA

    Woo-Suk Chang

  4. Department of Ecology Landscape Architecture-Design, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 54596, South Korea

    Yool-Jin Park

  5. Plant Medical Research Center, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Jeonbuk, 54896, South Korea

    Byung-Taek Oh

Authors
  1. Velu Manikandan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Palanivel Velmurugan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jung-Hee Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Woo-Suk Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yool-Jin Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Palaniyappan Jayanthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Min Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Byung-Taek Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Min Cho or Byung-Taek Oh.

Ethics declarations

Conflict of interest

The author(s) declare(s) that there is no conflict of interest regarding the publication of this paper.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Manikandan, V., Velmurugan, P., Park, JH. et al. Green synthesis of silver oxide nanoparticles and its antibacterial activity against dental pathogens. 3 Biotech 7, 72 (2017). https://doi.org/10.1007/s13205-017-0670-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-017-0670-4

Keywords

Search

Navigation