Abstract
In a one-step process, nanoscale magnetite was modified with 3-aminopropyltriethoxysilane to introduce amino groups onto its surface. The resulting nanocomposite exhibited a particle size of approximately 13.6 nm, with agglomerates measuring 150 nm in size. The nanoparticles were densely packed, creating gaps (pores) between them of up to 13 nm and a specific surface area of 114 m2⋅g−1. The nanocomposite possessed approximately 0.8 mmol⋅g−1 of amino groups. Electrokinetic potential data confirmed the presence of basic groups, and the isoelectric point was observed at pH 10. The adsorption of human immunoglobulin in a 0.9% NaCl solution was investigated using the synthesized nanoparticles, with a maximum adsorption capacity reaching 40.5 mg⋅g−1. Infrared spectroscopy data of the Ig-loaded sample and the fitting of the adsorption isotherm using the Langmuir adsorption model indicated chemisorption of the immunoglobulin on the surface of the Fe3O4/≡Si(CH2)3NH2 particles.
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This work was supported by the National Scholarship Programme of the Slovak Republic (ID 34561).
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The funding was supported by Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR, ID 34561, Nataliia Kusiak.
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Kusiak, N., Marcin Behunova, D., Yankovych, H. et al. Specific aspects of human immunoglobulin interactions with Fe3O4/≡Si(CH2)3NH2 nanocomposite surface. Appl Nanosci 13, 7219–7230 (2023). https://doi.org/10.1007/s13204-023-02883-6
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DOI: https://doi.org/10.1007/s13204-023-02883-6