Abstract
Simultaneous determination of small biologically important molecules is more significant for monitoring several diseases at physiological pH. Uric acid (C5H4N4O3) and ascorbic acid (C6H8O6), for instance, were once regarded as crucial components in the physiological processes of humans since they coexist in numerous biological matrices regularly, and monitoring their concentrations simultaneously indicates diseases like gout. In the present work, the gold nanoparticles (AuNPs) were assembled on a glassy carbon electrode (GCE) using electrodeposition of Au3+ ions for the simultaneous determination of C5H4N4O3 and C6H8O6. The deposited AuNPs were characterized using UV–visible spectroscopy, XPS, XRD, and AFM. Morphological analysis depicted that the particles are spherical with a size of 32 nm, and the particles are uniformly distributed on the electrode surface. The formation of gold oxide and its reduction peaks in cyclic voltammetry revealed the effective deposition of AuNPs on the GCE surface. The catalytic activity AuNPs modified GCE was analyzed by probing the oxidation of C5H4N4O3 and C6H8O6 at physiological pH (pH 7.2). The present modified electrode greatly enhanced the oxidation peak currents, and the potential of oxidation is also shifted to less positive potential compared to bare GCE. The AuNPs/GCE was used to determine C5H4N4O3 in the presence of a 50-fold high concentration of C6H8O6. The detection of 40 nM C5H4N4O3 was achieved using amperometry and a detection limit of 1.8 × 10–9 M (Signal/Noise = 3) was observed for the determination of uric acid in the concentration range from 4 × 10–8 to 4 × 10–5 M.
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Denisdon, S., Kumar, P.S., Jeevagan, A.J. et al. Simultaneous detection of uric and ascorbic acids by AuNPs electrodeposited on the GCE surface. Appl Nanosci 13, 5949–5958 (2023). https://doi.org/10.1007/s13204-023-02869-4
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DOI: https://doi.org/10.1007/s13204-023-02869-4