Abstract
Biological formulation of copper nanoparticles (Cu-Nps) in cancer treatment has augmented due to its beneficial and curative effects. Ziziphus zizyphus (ZZ) is a species of medicinal plant traditionally used for various ailments. Hence, this work deals with formulation of copper nanoparticles (Cu-NP’s) from Z. zizyphus leaf extracts (Cu-ZZ NP’s) and examine its effect on human renal carcinoma A498 cells. The characterization of the formulated Cu-ZZ NPs was done by spectroscopic and microscopic methods. The anti-cancer tests (MTT, ROS, MMP, Hoechst staining, comet, Real‐time PCR, and western blot) were used to evaluate the biological properties of Cu-ZZ NPs on the growth and proliferation of A498 cells. The cytotoxic activity of Cu-ZZ NPs resulted in potent toxicity of A498 cells. Further, Cu-ZZ NP’s triggered an intracellular ROS generation through mitochondrial pathways in the A498 cells, revealing a noteworthy cytotoxicity activity. Also, fluorescent microscopic studies using Hoechst staining, and comet analysis confirmed the occurrence of apoptosis in Cu-ZZ NPs treated A498 cells. Following, quantitative RT-PCR presented a negative regulation of Bcl-2 expression and positive regulation of Bid, Bax, and caspases (3 and 9) gene expression in Cu-ZZ NP’s treated A498 cells. To further justify, inhibition of mTOR/PI3K/AKT protein expression by Cu-ZZ NPs in A498 cells, lead to an inference that Cu-ZZ NPs induce apoptosis in A498 cells by inhibiting the mTOR, PI3K/Akt pathway.
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Li, X., Tang, G., Guo, X. et al. Characterization and apoptotic effect of copper nanoparticles biosynthesized from Ziziphus zizyphus leaf on human renal cell carcinoma A498 cells. Appl Nanosci 11, 139–148 (2021). https://doi.org/10.1007/s13204-020-01570-0
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DOI: https://doi.org/10.1007/s13204-020-01570-0