Abstract
The present work aims at studying the in vitro anti-diabetic activity of T. bellirica embedded gold nanoparticles synthesized using T. bellirica dried fruit and seed extract by alpha-amylase assay. Gold nanoparticles were prepared from various green synthesis routes with T. bellirica aqueous extracts. The products are well characterized by Ultraviolet–Visible spectroscopy (UV–Vis), Field Emission Scanning Electron Microscopy (FESEM), Fourier-Transform Infrared spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). Gold nanoparticles of violet–pink colour were synthesized by five different methods. Optimization of reaction conditions was also carried out. The biosynthesized gold nanoparticles (TBNg 150 µl) showed good inhibition efficiency (46 and 42%) against Aspergillus oryzae and Porcine pancreas, respectively, compared to the standard acarbose (60.71 and 61.90%, respectively). Microwave-assisted synthesis gave T. bellirica mediated gold nanoparticles with less time (3:00 and 3:80 s) for the ratio of plant extract and gold solution 5:1 and 3:1, respectively. The UV–Visible absorption spectral analysis of synthesized nano exhibited Surface Plasmon Resonance at 560 nm range indicating the formation of gold nanoparticles. TEM results revealed various shapes of nanogold namely spherical, hexagonal and triangular shaped gold nanoparticles. The smallest size of nanoparticle synthesized was 5.1 nm. The results also evidence, alpha-amylase assay as a facile screening tool for assessing the anti-diabetic activity of plant extracts.
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References
Balfour JA, McTavish D (1993) Acarbose: an update of its pharmacology and therapeutic use in diabetes mellitus. Drugs 46:1025–1054. https://doi.org/10.2165/00003495-199346060-00007
Chiasson J-L, Josse RG, Gomis R et al (2002) Acarbose for prevention of type 2 diabetes mellitus: the STOP-NIDDM randomised trial. Lancet 359:2072–2077. https://doi.org/10.1016/S0140-6736(02)08905-5
Delorme S, Chiasson J-L (2005) Acarbose in the prevention of cardiovascular disease in subjects with impaired glucose tolerance and type 2 diabetes mellitus. Curr Opin Pharmacol 5:184–189. https://doi.org/10.1016/j.coph.2004.11.005
Fatima I, Waheed S, Zaidi JH (2013) Elemental analysis of Anethum gravedlens, Sismbrium Irio Linn and Veronia Anthelmintica seeds by instrumental neutron activation analysis. Appl Radiat Isot 71:57–61. https://doi.org/10.1016/j.apradiso.2012.09.022
Firdhouse J, Lalitha P (2015) Maestro 9.4 as a tool in the structure based screening of glycoalkaloids and related compounds, targeting aldose reductase. Trend Bioinform. https://doi.org/10.3923/tb.2015.26.36
Firdhouse J, Lalitha P, Shubashini S (2014) An undemanding method of synthesis of gold nanoparticles using pisonia grandis (R. Br.). Dig J Nanomater Biostructures 9:385–393
Freitas RA Jr (1999) Nanomedicine, volume I: basic capabilities. Landes Bioscience, Georgetown
Funke I, Melzig M (2006) Traditionally used plants in diabetes therapy—phytotherapeutics as inhibitors of α-amylase activity. Rev Bras Farmacogn. https://doi.org/10.1590/S0102-695X2006000100002
Kumar N, Khurana SP (2018) Phytochemistry and medicinal potential of the Terminalia bellirica Roxb. (Bahera). Indian J Nat Prod Resour 9:97–107
Laube H (2002) Acarbose: an update of its therapeutic use in diabetes treatment. Clin Drug Investig 22:141–156
Rahmatullah M, Chowdhury M, Jahan R (2009) A survey of medicinal plants in two areas of Dinajpur District, Bangladesh including plants which can be used as functional foods. Am J Sustain Agric 3:862–876
Rathor L, Pant A, Awasthi H et al (2017) An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology 18:131–147. https://doi.org/10.1007/s10522-016-9668-2
Saha M (2009) Nanomedicine: promising tiny machine for the healthcare in future-a review. Oman Med J 24:242–247. https://doi.org/10.5001/omj.2009.50
Sanjay SS, Pandey AC (2017) A brief manifestation of nanotechnology. In: Shukla AK (ed) EMR/ESR/EPR spectroscopy for characterization of nanomaterials. Springer India, New Delhi, pp 47–63
Saraswathi MN, Karthikeyan M, Kannan M, Rajasekar S (2012) Terminalia belerica .Roxb-A phytopharmacological review. Int J Res Pharm Biomed Sci 3:96–99. http://www.mchemist.com/ayas/pdf/10%20bibhitaki.pdf
Tanaka M, Kishimoto Y, Saita E et al (2016) Terminalia bellirica extract inhibits low-density lipoprotein oxidation and macrophage inflammatory response in vitro. Antioxidants (Basel, Switzerland) 5:20. https://doi.org/10.3390/antiox5020020
Viswadevarayalu A, Posa V, Sreenivasa Kumar G et al (2016a) Fine ultrasmall copper nanoparticle (UCuNPs) synthesis by using terminalia bellirica fruit extract and its antimicrobial activity. J Clust Sci. https://doi.org/10.1007/s10876-015-0917-3
Viswadevarayalu A, Posa V, Viaya Lakshmi D et al (2016b) Terminalia bellirica Fruit Extract Mediated Synthesis of Gold Nanoparticles (AuNPs) and studies on Antimicrobial and Antioxidant activity. Synth React Inorg Met-Organ Nano-Met Chem. https://doi.org/10.1080/15533174.2016.1212219
Waheed S, Fatima I (2013) Instrumental neutron activation analysis of Emblica officinalis, Terminalia belerica and Terminalia chebula for trace element efficacy and safety. Appl Radiat Isot 77:139–144. https://doi.org/10.1016/j.apradiso.2013.03.007
Wehmeier UF, Piepersberg W (2004) Biotechnology and molecular biology of the α-glucosidase inhibitor acarbose. Appl Microbiol Biotechnol 63:613–625. https://doi.org/10.1007/s00253-003-1477-2
Xue Y-P, Qin J-W, Wang Y-J et al (2013) Enhanced production of acarbose and concurrently reduced formation of impurity C by addition of validamine in fermentation of Actinoplanes utahensis ZJB-08196. Biomed Res Int 2013:705418. https://doi.org/10.1155/2013/705418
Zhang Z, Huo F, Zhang X, Guo D (2012) Fabrication and size prediction of crystalline nanoparticles of silicon induced by nanogrinding with ultrafine diamond grits. Scr Mater 67:657–660. https://doi.org/10.1016/j.scriptamat.2012.07.016
Zhang Z, Guo D, Wang B et al (2015a) A novel approach of high speed scratching on silicon wafers at nanoscale depths of cut. Sci Rep 5:16395. https://doi.org/10.1038/srep16395
Zhang Z, Wang B, Kang R et al (2015b) Changes in surface layer of silicon wafers from diamond scratching. CIRP Ann 64:349–352. https://doi.org/10.1016/j.cirp.2015.04.005
Zhang Z, Wang B, Zhou P et al (2016a) A novel approach of chemical mechanical polishing for cadmium zinc telluride wafers. Sci Rep 6:26891. https://doi.org/10.1038/srep26891
Zhang Z, Wang B, Zhou P et al (2016b) A novel approach of chemical mechanical polishing using environment-friendly slurry for mercury cadmium telluride semiconductors. Sci Rep 6:22466. https://doi.org/10.1038/srep22466
Zhang Z, Du Y, Wang B et al (2017a) Nanoscale Wear Layers on Silicon Wafers Induced by Mechanical Chemical Grinding. Tribol Lett 65:132. https://doi.org/10.1007/s11249-017-0911-z
Zhang Z, Huang S, Wang S et al (2017b) A novel approach of high-performance grinding using developed diamond wheels. Int J Adv Manuf Technol 91:3315–3326. https://doi.org/10.1007/s00170-017-0037-3
Zhang Z, Shi Z, Du Y et al (2018) A novel approach of chemical mechanical polishing for a titanium alloy using an environment-friendly slurry. Appl Surf Sci 427:409–415. https://doi.org/10.1016/j.apsusc.2017.08.064
Zhang Z, Cui J, Zhang J et al (2019) Environment friendly chemical mechanical polishing of copper. Appl Surf Sci 467–468:5–11. https://doi.org/10.1016/j.apsusc.2018.10.133
Zhang Z, Liao L, Wang X et al (2020) Development of a novel chemical mechanical polishing slurry and its polishing mechanisms on a nickel alloy. Appl Surf Sci 506:144670. https://doi.org/10.1016/j.apsusc.2019.144670
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The authors would like to acknowledge the Avinashilingam Institute for Home Science and Higher Education for Women University, Coimbatore, Tamil Nadu, for providing research facilities.
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Smina, C.S., Lalitha, P., Nagabhushana, H. et al. Terminalia bellirica dried fruit and seed extract offers alpha-amylase inhibitory potential in tackling diabetes. Appl Nanosci 10, 4325–4339 (2020). https://doi.org/10.1007/s13204-020-01549-x
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DOI: https://doi.org/10.1007/s13204-020-01549-x