Abstract
The main aim of the present study was the investigation of the viability of two probiotics bacteria of Lactobacillus acidophilus and Lactobacillus plantarum in different environments including simulated gastrointestinal and circumstances namely yogurt. For coating of strains, the double-coating technique was used as the first coating was alginate chitosan and second was Eudragit S100 and the results compared with free-coating cells. the number of free L. acidophilus and L. plantarum were 6.4 × 107 ± 3.3 × 104 and 7.5 × 107 ± 3.5 × 10, respectively, and after 32 days in the last measurement, a sharp decrease in the numbers was observed as they reached to 4.6 × 104 ± 0.9 × 102 and 1.5 × 102 ± 7.9 × 101, respectively. In double-coated condition more number of strains survived as L. acidophilus and L. plantarum were 6.8 × 107 ± 1.8 × 105 and 7.3 × 107 ± 2.8 × 105 at the first measurement, respectively, and at the end of counting the numbers were 5.7 × 105 ± 8.8 × 102 and 9.8 × 104 ± 8.8 × 101. The evaluation of the viability in a wide range of pH shows that two strains survive more in both free and double-coated form, however, L. acidophilus performed better. The results indicating that microencapsulation play as a protective role and strains survive longer than free-coating cells. This method is effective in increasing the viability of beneficial bacteria, so it can bring many benefits to the human body.
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Abbreviations
- GI:
-
Gastrointestinal
- Eu:
-
Eudragit
- SAS:
-
Supercritical antisolvent technique
- PDI:
-
Polydispersity index
- CFU:
-
Colony forming units
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Rahmati, F. Microencapsulation of Lactobacillus acidophilus and Lactobacillus plantarum in Eudragit S100 and alginate chitosan under gastrointestinal and normal conditions. Appl Nanosci 10, 391–399 (2020). https://doi.org/10.1007/s13204-019-01174-3
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DOI: https://doi.org/10.1007/s13204-019-01174-3