In vitro anticancer evaluation of chitosan/biogenic silver nanoparticle conjugate on Si Ha and MDA MB cell lines

  • Smitha Vijayan
  • K. Divya
  • M. S. JishaEmail author
Original Article


Nanomedicine is an innovative approach to cancer therapy. The present study is in vitro anticancer evaluation of chitosan-stabilized biogenic silver nanoparticles (Ch/Bio-AgNPs) on Si Ha (human cervical carcinoma) and MDA MB (human adenocarcinoma) cells. IC50 values of Bio-AgNP and Ch/Bio-AgNP were studied by MTT assay, antiproliferative and cell apoptosis-inducing activities of the conjugate were examined by ethidium bromide/acridine orange double staining, RO analysis, DNA fragmentation, Caspase 7 and 9 assays and flow cytometry. Gene expression was studied by real-time PCR using the β-actin housekeeping gene. As a result, on MDA MB cells IC50 value for Bio-AgNP and Ch/Bio-AgNP was 4.346 ± 0.6381 µg/ml and 0.9851 ± 0.0065 µg/ml, respectively, and on Si Ha cells it was 24.35 ± 1.390 µg/ml and 2.86 ± 0.319 µg/ml, respectively. Ch/Bio-AgNPs was a potent inhibitor on MDA MB and Si Ha cell lines with characteristic apoptosis and up-regulation of p53 and p38 genes.


Chitosan/Bio-AgNP conjugate Si Ha cells MDA MB cells Antitumor activity Flow cytometry 



This work was supported by Mahatma Gandhi University (Section Order no: 529/A6/2/JRF2018-2019/Academic), Kottayam, Kerala, India. I also acknowledge Business Innovation and Incubation Centre (BIIC), Mahatma Gandhi University, for Patent Facilitation and Start-up award.

Compliance with ethical standards

Conflict of interest

Authors state that there is no conflict of interest.

Supplementary material

13204_2019_1151_MOESM1_ESM.docx (246 kb)
Supplementary material 1 (DOCX 245 kb)


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© King Abdulaziz City for Science and Technology 2019

Authors and Affiliations

  1. 1.School of BiosciencesMahatma Gandhi UniversityKottayamIndia

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