Abstract
Zinc oxide (ZnO) in vitro antifungal activity with three particle shapes were studied. These morphologies were obtained by colloidal and hydrothermal synthesis techniques, the synthesis parameters and methodology for both systems were optimized to obtain particles with the following shapes: nanoparticles, lamellar platelets and hexagonal rods. Morphology and particle size distribution were determined by electron microscopy imaging techniques. The antifungal activity of each type of ZnO particles was evaluated for three phytopathogenic fungi species; Fusarium oxysporum f.sp. lycopersici, Fusarium solani, and Colletotrichum gloeosporioids. ZnO with platelets shaped particles have better antifungal inhibition activity than rods and nanoparticles and it reduced growth up to 65% against Fusarium solani.
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References
Agrios GN (1988a) Introduction to plant pathology. Plant pathology. Elsevier, Amsterdam, pp 3–39
Agrios GN (1988b) Plant diseases caused by fungi. Plant pathology. Elsevier, Amsterdam, pp 265–509
Arciniegas-Grijalba PA, Patiño-Portela MC, Mosquera-Sánchez LP et al (2017) ZnO nanoparticles (ZnO–NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor. Appl Nanosci 7:225–241. https://doi.org/10.1007/s13204-017-0561-3
Arciniegas-Grijalba PA, Patiño-Portela MC, Mosquera-Sánchez LP et al (2019) ZnO-based nanofungicides: synthesis, characterization and their effect on the coffee fungi Mycena citricolor and Colletotrichum sp. Mater Sci Eng C 98:808–825. https://doi.org/10.1016/j.msec.2019.01.031
Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 136:71–79. https://doi.org/10.1016/j.jpha.2015.11.005
Cross SE, Innes B, Roberts MS et al (2007) Human skin penetration of sunscreen nanoparticles: in-vitro assessment of a novel micronized zinc oxide formulation. Skin Pharmacol Physiol 20:148–154. https://doi.org/10.1159/000098701
Doehlemann G, Bilal Ö, Zhu W, Sharon A (2017) Plant Pathogenic Fungi. Microbiol Spectr 5:1–23. https://doi.org/10.1128/microbiolspec.FUNK-0023-2016.Correspondence
Espitia PJP, Soares NDFF, dos Reis Coimbra JS et al (2012) Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications. Food Bioprocess Technol 5:1447–1464. https://doi.org/10.1007/s11947-012-0797-6
Feliziani Erica, Lucia Landi GR (2015) Preharvest treatments with chitosan and other alternatives to conventional fungicides to control postharvest decay of strawberry. Carbohydr Polym 132:111–117
Fu PP, Xia Q, Hwang HM et al (2014) Mechanisms of nanotoxicity: generation of reactive oxygen species. J Food Drug Anal 22:64–75. https://doi.org/10.1016/j.jfda.2014.01.005
Hae-Jun P, Kim SH, Kim HJ, Choi S-H (2006) A new composition of nanosized silica-silver for control of various plant diseases. Plant Pathol J 22(3):295–302
He L, Liu Y, Mustapha A, Lin M (2011) Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiol Res 166:207–215. https://doi.org/10.1016/j.micres.2010.03.003
He W, Zhao H, Jia H et al (2014) Determination of reactive oxygen species from ZnO micro-nano structures with shape-dependent photocatalytic activity. Mater Res Bull 53:246–250. https://doi.org/10.1016/j.materresbull.2014.02.020
Jones N, Ray B, Ranjit KT, Manna AC (2008) Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett 279:71–76. https://doi.org/10.1111/j.1574-6968.2007.01012.x
Kanhed P, Birla S, Gaikwad S et al (2014) In vitro antifungal efficacy of copper nanoparticles against selected crop pathogenic fungi. Mater Lett 115:13–17. https://doi.org/10.1016/j.matlet.2013.10.011
Kislov N, Lahiri J, Verma H et al (2009) Photocatalytic degradation of methyl orange over single crystalline ZnO: orientation dependence of photoactivity and photostability of ZnO. Langmuir 25:3310–3315. https://doi.org/10.1021/la803845f
Koul O (2019) Nano-biopesticides today and future perspectives. Academic Press, San Diego
Landi L, Feliziani E, Romanazzi G (2014) Expression of defense genes in strawberry fruits treated with different resistance inducers. J Agric Food Chem 62:3047–3056. https://doi.org/10.1021/jf404423x
Ma H, Williams PL, Diamond SA (2013) Ecotoxicity of manufactured ZnO nanoparticles e A review. Environ Pollut 172:76–85
Malandrakis AA, Kavroulakis N, Chrysikopoulos CV (2019) Use of copper, silver and zinc nanoparticles against foliar and soil-borne plant pathogens. Sci Total Environ 670:292–299. https://doi.org/10.1016/j.scitotenv.2019.03.210
Montes-Fonseca F, Olive-Méndez SF, Holguín-Momaca JT et al (2017) Role of oxygen vacancies and In-doping on the sensing performance of ZnO particles. Phys Status Solidi C 1600226:1–6. https://doi.org/10.1002/pssc.201600226
Morales-Mendoza JE, Paraguay-Delgado F, Moller JAD et al (2019) Structure and optical properties of ZnO and ZnO2 nanoparticles. J Nano Res 56:49–62. https://doi.org/10.4028/www.scientific.net/JNanoR.56.49
Nasrin T, Mohammad Reza N, Elahe Badri Z (2011) Enhanced antibacterial performance of hybrid semiconductor nanomaterials: znO/SnO2 nanocomposite thin films. Appl Surf Sci 258:547–555
Nasrin T, Seyedeh Matin A, Monir D (2013) Controllable synthesis of ZnO nanoparticles and their morphology-dependent antibacterial and optical properties. Photochem Photobiol 120:66–73
Pariona N, Mtz-Enriquez AI, Sánchez-Rangel D et al (2019) Green-synthesized copper nanoparticles as a potential antifungal against plant pathogens. RSC Adv 9:18835–18843. https://doi.org/10.1039/C9RA03110C
Peng X, Palma S, Fisher NS, Wong SS (2011) Effect of morphology of ZnO nanostructures on their toxicity to marine algae. Aquat Toxicol 102:186–196. https://doi.org/10.1016/j.aquatox.2011.01.014
Pimentel David, McLaughlin Lori, Zepp Andrew, Benyamin Lakitan T, Kraus Peter Kleinman, Fabius Vancini W, John Roach EG, William S, Keeton GS (1993) Environmental and economic effects of reducing pesticide use in agriculture. Agric Ecosyst Environ 46:273–288
Prasad R (2016) Advances and applications through fungal nanobiotechnology. Springer, New York
Price CL, Parker JE, Warrilow AG et al (2015) Azole fungicides - understanding resistance mechanisms in agricultural fungal pathogens. Pest Manag Sci 71:1054–1058. https://doi.org/10.1002/ps.4029
Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 27:76–83. https://doi.org/10.1016/j.biotechadv.2008.09.002
Romashchenko AV, Kan T-W, Petrovski DV et al (2017) Nanoparticles associate with intrinsically disordered RNA-binding proteins. ACS Nano 11:1328–1339. https://doi.org/10.1021/acsnano.6b05992
Saharana Vinod, Sharma Garima, Meena Yadav MKC, Sharma SS, Pal Ajay, Ramesh Raliya PB (2015) Synthesis and in vitro antifungal efficacy of Cu–chitosan nanoparticles against pathogenic fungi of tomato. Int J Biol Macromol 75:346–353
Shahid S, Mudassar Sher M (2017) Solvothermal synthesis and biological activity of ni-doped zinc oxide nanoparticles. Press Procedia. https://doi.org/10.17261/pressacademia.2017.595
Shang L, Nienhaus K, Nienhaus G (2014) Engineered nanoparticles interacting with cells: size matters. J Nanobiotechnol 12:5. https://doi.org/10.1186/1477-3155-12-5
Sharma RK, Agarwal M, Balani K (2016) Effect of ZnO morphology on affecting bactericidal property of ultra high molecular weight polyethylene biocomposite. Mater Sci Eng C 62:843–851. https://doi.org/10.1016/j.msec.2016.02.032
Shi L-E, Li Z-H, Zheng W et al (2014) Synthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: a review. Food Addit Contam Part A 31:173–186. https://doi.org/10.1080/19440049.2013.865147
Stankovic A, Dimitrijevic S, Uskoković D (2013) Influence of size scale and morphology on antibacterial properties of ZnO powders hydrothemally synthesized using different surface stabilizing agents. Colloids Surfaces B Biointerfaces 102:21–28
Sun Q, Li J, Le T (2018) Zinc oxide nanoparticle as a novel class of antifungal agents: current advances and future perspectives. J Agric Food Chem 66:11209–11220. https://doi.org/10.1021/acs.jafc.8b03210
Thabet S, Simonet F, Lemaire M, Guillard C (2014) Impact of photocatalysis on fungal cells: depiction of cellular and molecular effects on Saccharomyces cerevisiae. Appl Environ Microbiol 80:7527–7535. https://doi.org/10.1128/AEM.02416-14
Wang YX, Sun J, Yu X (2011) Effect of the type of alcohol on the properties of zno nanopowders prepared with solvothermal synthesis. Mater Sci Forum 663–665:1103–1106. https://doi.org/10.4028/www.scientific.net/msf.663-665.1103
Woo SL, Ruocco M, Vinale F, et al (2014) Trichoderma-based Products and their Widespread Use in Agriculture
Yang J, Hsiang T, Bhadauria V et al (2017) Plant fungal pathogenesis. Biomed Res Int 2017:1–2. https://doi.org/10.1155/2017/9724283
Acknowledgements
This work was supported by FORDECYT 292399. We would like to thank W. Antunez, C. Ornelas, D. Lardizabal and E. Guerrero for their technical help at NaNoTech, Cimav, Chihuahua. Also, we would like to thank Zelene Duran Barradas for the kindly provide and preparation of the fungal suspensions.
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Pariona, N., Paraguay-Delgado, F., Basurto-Cereceda, S. et al. Shape-dependent antifungal activity of ZnO particles against phytopathogenic fungi. Appl Nanosci 10, 435–443 (2020). https://doi.org/10.1007/s13204-019-01127-w
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DOI: https://doi.org/10.1007/s13204-019-01127-w