Preparation of carboxy-methyl cellulose-capped nanosilver particles and their antimicrobial evaluation by an automated device
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Colloidal solution of nano silver particles (AgNPs) have been prepared using carboxymethyl cellulose as the stabilizing agent and dextrose as the reducing agent. It is considered bio-friendly, as all ingredients at much higher concentrations are used on eye as medicine. AgNPs thus generated are triangular with 9.5 nm size. MIC values of AgNPs and equivalent ionic silver against different multi-drug resistant strain bacteria and yeast are determined by microdilution method. Biological parameters for nano conversion are indicated by 128–256-fold higher sensitivity. Selective range synergisms for 1/4th MIC AgNPs with battery of antimicrobial agents are indicated by automated susceptibility testing device, keeping a negative control. Much lower MICs for different resistant antibiotics in combination with AgNPs are noted for all test organisms. This indicates scope for using a tolerable concentration of nanoantimicrobials either alone or in combination with an empirically chosen antibiotic for managing surface infections of eye or skin.
KeywordsSilver nanoparticles Carboxy-methyl cellulose Synergism Topical anti-microbial Automated susceptibility testing
PKM conceived the project and designed the experiments dealing with synthesis, storage of eye tolerable AgNPs and new methods for determining anti-microbial properties. AG and RP carried out experiments dealing with synthesis, characterizations and microbiological experiments with AgNPs. MRC, AG and AS carried out the electron microscopic studies and experiments related to physical characterization of AgNPs. All authors were involved with interpretation of obtained results, preparation and editing the manuscript.
Compliance with ethical standards
Conflict of interest
Applied for Patent in India by Prasanta Kumar Maiti; Application no. 201831009290, Date of publication 6th, April, 2018.
- Alahmadi NS, Betts JW, Heinze T, Kelly SM, Koschella A, Wadhawan JD (2018) Synthesis and antimicrobial effects of highly dispersed, cellulose-stabilized silver/cellulose nanocomposites. RSC Adv 8: 3646–3656. https://doi.org/10.1039/c7ra12280brsc.li/rsc-advances CrossRefGoogle Scholar
- Lustosa AKMF, de Jesus Oliveira AC, Quelemes PV, Plácido A, da Silva FV, Oliveira IS et al (2017) In situ synthesis of silver nanoparticles in a hydrogel of carboxymethyl cellulose with phthalated-cashew gum as a promising antibacterial and healing agent. Int J Mol Sci. https://doi.org/10.3390/ijms18112399 Google Scholar
- Maiti PK, Haldar J, Mukherjee P, Dey R (2013) Anaerobic culture on growth efficient bi-layered culture plate in a modified candle jar using a rapid and slow combustion system. Indian J Med Microbiol 31:173–176Google Scholar
- Mims JL Jr (1951) Methyl cellulose solution for ophthalmic use. AMA Arch Ophthalmol 46(6):664–665. https://doi.org/10.1001/archopht.1951.01700020678008 CrossRefGoogle Scholar
- Ordzhonikidze CG, Ramaiyya LK, Egorova EM, Rubanovich AV (2009) Genotoxic effects of silvernanoparticles on mice in vivo. Actanaturae 3:99–101Google Scholar
- PanáˇcekA SmékalováM, KilianováM PrucekR, BogdanováK VeˇceˇrováR et al (2016) Strong and nonspecific synergistic antibacterial efficiency of antibiotics combined with silver nanoparticles at very low concentrations showing no cytotoxic effect. Molecules 21:26. https://doi.org/10.3390/molecules21010026 CrossRefGoogle Scholar
- Waris A, Nagpal G, Akhtar N (2014) Use of nanotechnology in ophthalmology. Am J Drug Deliv Ther 1(2):73–76Google Scholar
- You C, Han C, Wang X, Zheng Y, Li Q, Hu X, Sun H (2012) The progress of silver nanoparticles in the antibacterial mechanism, clinical application and cytotoxicity. Mol Biol Rep 39:9193–9201. https://doi.org/10.1007/s11033-012-1792-8PMID:22722996 CrossRefGoogle Scholar