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Prolonged and continuous antibacterial and anti-biofilm activities of thin films embedded with gentamicin-loaded mesoporous silica nanoparticles

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Abstract

The application of mesoporous silica nanoparticles (MSNs) in drug delivery systems has become highly attractive since the early 2000s. In this study, thin-film coatings embedded with gentamicin-loaded mesoporous silica nanoparticles (MSN-G) were prepared to provide antibacterial and anti-biofilm activity over a prolonged period of time. The prolonged and continuous activity of MSN-G films against Staphylococcus aureus throughout the release period was studied via two methods, namely, (1) disc diffusion of released gentamicin and (2) by shifting the MSN-G thin film to a new agar plate at certain time intervals. The expansion of the inhibition zone from 4.6 ± 0.5 to 9.7 ± 0.5 mm as caused by the released fraction of gentamicin from the first week to the eighth week indicated the controlled and slow release behaviour of loaded antibiotic and prolonged antibacterial efficacy of these films. In addition, the appearance of an inhibition zone after each shifting of the film to a new agar plate was persistent up to 103 days which confirmed that thin films successively prevented bacterial growth over a long period of time. In addition, the anti-biofilm activity of MSN-G films was evaluated by imaging bacterial cells attachment via confocal laser scanning microscopy and scanning electron microscopy. Remarkably, the anti-biofilm performance remained active for more than 2 months. To the best of our knowledge, such a slow and controlled release of antibiotic from nanoparticle embedded thin films with uninterrupted, continuous, and prolonged antibacterial effect for more than 2 months has not been reported yet.

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Acknowledgements

T. Tamanna acknowledges Australian Government Research Training Program Scholarship for supporting this work. C. B. Landersdorfer is an Australian National Health and Medical Research Council (NHMRC) Career Development Fellow (APP1062509).

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Authors and Affiliations

  1. Faculty of Science, Engineering and Technology, Swinburne University of Technology, John Street, Hawthorn, VIC, 3122, Australia

    Tasnuva Tamanna, Hooi Jun Ng, Peter Wood & Aimin Yu

  2. Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Australia

    Cornelia B. Landersdorfer

  3. Centre for Medicine Use and Safety, Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, Parkville, Australia

    Cornelia B. Landersdorfer

  4. Center for Pharmacometrics and Systems Pharmacology, College of Pharmacy, University of Florida, Orlando, USA

    Jürgen B. Bulitta

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  1. Tasnuva Tamanna
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  2. Cornelia B. Landersdorfer
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  3. Hooi Jun Ng
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  4. Jürgen B. Bulitta
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  5. Peter Wood
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  6. Aimin Yu
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Correspondence to Aimin Yu.

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Tamanna, T., Landersdorfer, C.B., Ng, H.J. et al. Prolonged and continuous antibacterial and anti-biofilm activities of thin films embedded with gentamicin-loaded mesoporous silica nanoparticles. Appl Nanosci 8, 1471–1482 (2018). https://doi.org/10.1007/s13204-018-0807-8

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