New hybrid composites for photodynamic therapy: synthesis, characterization and biological study
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Photodynamic therapy is a procedure that uses a photosensitizing drug to apply light therapy selectively to target cancer treatment. This study is focused on a synthesis and characterization of a new hybrid nanocomposites based on the branched copolymers dextran–polyacrylamide in nonionic, D-g-PAA and anionic D-g-PAA(PE) form, with incorporated gold nanoparticles (AuNPs) and photosensitizer chlorin e6 (Ce6) simultaneously. Double polymer/AuNPs and trial polymer/AuNPs/Ce6 were studied by TEM, UV–visible, SOSG fluorescence. It was found the drastic difference for absorbance for trial nanosystems synthesized in nonionic and anionic polymers matrices. It was established that for the nanocomposite synthesised in anionic polymer matrix with the Ce6:Au mass ratio 1:10 generation of singlet oxygen (1O2) was quite close to that for free Ce6. The study of ability of this nanosystem to sensitize MT-4 cells to photodynamic damage has shown that the nanocomposite, that contained AuNPs during the synthesis of which HAuCl4:NaBH4 mass ratio was 1:2 showed higher photodynamic activity, than Ce6 itself. Nanosystem D70-g-PAA(PE)/AuNPs/Ce6 can be recommended to experiment in vivo.
KeywordsNanocomposite Star-like copolymer Gold nanoparticle Photosensitizer Photodynamic activity
This paper is dedicated to the memory of Professor Mykola F. Gamaliya (Gamaleya)—the Head of the Laboratory of Quantum Nanobiology at Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, who was a founder of the laser therapy and photodynamic therapy of tumors in Ukraine. This publication is supported in part by the Grant of the State Fund For Fundamental Research (Ukraine), project Ф76/64-2017 “New multifunctional hybrid nanocomposites for photodynamic chemotherapy of tumor cells” and by the grant of the Department of Targeted Training of Taras Shevchenko National University of Kyiv at National Academy of Sciences of Ukraine, project 28Ф “Physical mechanisms of the oxygen molecules excitation in functional nanosystems for medical application”.
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