Advertisement

Applied Nanoscience

, Volume 8, Issue 3, pp 347–357 | Cite as

Synthesis of curcumin-functionalized gold nanoparticles and cytotoxicity studies in human prostate cancer cell line

  • Shruti Nambiar
  • Ernest Osei
  • Andre Fleck
  • Johnson Darko
  • Anthony J. Mutsaers
  • Shawn Wettig
Original Article

Abstract

Gold nanoparticles synthesized using plant extracts with medicinal properties have gained traction in recent years, especially for their use in various biomedical applications. Colloidal stability of these nanoparticles in different environments is critical to retain the expected therapeutic/diagnostic efficacy and toxicological outcome. Any change in the colloidal stability leads to dramatic changes in the physico-chemical properties of the nanoparticles such as size and surface charge, which in turn may alter the biological activity of the particles. Such changes are imminent in physiologically-relevant environment wherein interactions with different biomolecules, such as serum proteins, may modify the overall properties of the nanoparticles. In this regard, we synthesized 15 nm sized gold nanoparticles using curcumin, a plant extract from turmeric root, to evaluate cytotoxicity, uptake, and localization in human prostate cancer cells using cell-culture medium supplemented with or without fetal bovine serum (FBS). The results indicate a dramatic difference in the cytotoxicity and uptake between cells treated with curcumin-functionalized gold nanoparticles (cur-AuNPs) in cell-culture medium with and without serum. The addition of FBS to the medium not only increased the stability of the nanoparticles but also enhanced the biocompatibility (i.e. minimal cytotoxicity for a wide range of cur-AuNP concentrations). We conclude that the presence of serum proteins significantly impact the therapeutic potential of cur-AuNPs.

Keywords

Gold nanoparticles Curcumin Prostate cancer Serum protein Cytotoxicity Cellular uptake 

Notes

Acknowledgements

This research was financially supported by the Telus Ride for Dad and the Prostate Cancer Fight Foundation. We are thankful to Prof. Jonathan Blay for his help with the phase contrast microscope.

Supplementary material

13204_2018_728_MOESM1_ESM.docx (25 kb)
Supplementary material 1 (DOCX 24 kb)

References

  1. Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB (2007) Bioavailability of curcumin: problems and promises. Mol Pharm 4:807–818CrossRefGoogle Scholar
  2. Boisselier E, Astruc D (2009) Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem Soc Rev 38:1759–1782.  https://doi.org/10.1039/b806051g CrossRefGoogle Scholar
  3. Coates JP (1996) The interpretation of infrared spectra: published reference sources. Appl Spectrosc Rev 31:179–192CrossRefGoogle Scholar
  4. Das RK, Sharma P, Nahar P, Bora U (2011) Synthesis of gold nanoparticles using aqueous extract of Calotropis procera latex. Mater Lett 65:610–613.  https://doi.org/10.1016/j.matlet.2010.11.040 CrossRefGoogle Scholar
  5. Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA (2012) The golden age: gold nanoparticles for biomedicine. Chem Soc Rev 41:2740–2779CrossRefGoogle Scholar
  6. Fröhlich E (2012) The role of surface charge in cellular uptake and cytotoxicity of medical nanoparticles. Int J Nanomed 7:5577–5591CrossRefGoogle Scholar
  7. Gangwar RK et al (2012) Conjugation of curcumin with PVP capped gold nanoparticles for improving bioavailability. Mater Sci Eng C 32:2659–2663CrossRefGoogle Scholar
  8. Hatcher H, Planalp R, Cho J, Torti FM, Torti SV (2008) Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci 65:1631–1652.  https://doi.org/10.1007/s00018-008-7452-4 CrossRefGoogle Scholar
  9. Hotze EM, Phenrat T, Lowry GV (2010) Nanoparticle aggregation: challenges to understanding transport and reactivity in the environment. J Environ Qual 39:1909–1924CrossRefGoogle Scholar
  10. Jain S, Hirst DG, O’Sullivan JM (2012) Gold nanoparticles as novel agents for cancer therapy. Br J Radiol 85:101–113.  https://doi.org/10.1259/bjr/59448833 CrossRefGoogle Scholar
  11. Kittler S et al (2010) The influence of proteins on the dispersability and cell-biological activity of silver nanoparticles. J Mater Chem 20:512–518CrossRefGoogle Scholar
  12. Levchenko LA et al (2011) Synthesis and study of gold nanoparticles stabilized by bioflavonoids. Russ Chem Bull 60:426.  https://doi.org/10.1007/s11172-011-0067-1 CrossRefGoogle Scholar
  13. Mahl D, Greulich C, Meyer-Zaika W, Köller M, Epple M (2010) Gold nanoparticles: dispersibility in biological media and cell-biological effect. J Mater Chem 20:6176–6181CrossRefGoogle Scholar
  14. Manju S, Sreenivasan K (2012) Gold nanoparticles generated and stabilized by water soluble curcumin–polymer conjugate: blood compatibility evaluation and targeted drug delivery onto cancer cells. J Colloid Interface Sci 368:144–151CrossRefGoogle Scholar
  15. Moore TL et al (2015) Nanoparticle colloidal stability in cell culture media and impact on cellular interactions. Chem Soc Rev 44:6287–6305CrossRefGoogle Scholar
  16. Nune SK et al (2009) Green nanotechnology from tea: phytochemicals in tea as building blocks for production of biocompatible gold nanoparticles. J Mater Chem 19:2912–2920CrossRefGoogle Scholar
  17. Parida UK, Bindhani BK, Nayak P (2011) Green synthesis and characterization of gold nanoparticles using onion (Allium cepa) extract. World J Nano Sci Eng 1:93CrossRefGoogle Scholar
  18. Priyadarsini KI (2014) The chemistry of curcumin: from extraction to therapeutic agent. Molecules 19:20091–20112CrossRefGoogle Scholar
  19. Sanna V et al (2014) Single-step green synthesis and characterization of gold-conjugated polyphenol nanoparticles with antioxidant and biological activities. Int J Nanomed 9:4935Google Scholar
  20. Shukla R et al (2008) Soybeans as a phytochemical reservoir for the production and stabilization of biocompatible gold nanoparticles. Small 4:1425–1436CrossRefGoogle Scholar
  21. Sindhu K, Rajaram A, Sreeram K, Rajaram R (2014) Curcumin conjugated gold nanoparticle synthesis and its biocompatibility. RSC Adv 4:1808–1818Google Scholar
  22. Singh C, Baboota RK, Naik PK, Singh H (2012) Biocompatible synthesis of silver and gold nanoparticles using leaf extract of Dalbergia sissoo. Adv Mater Lett 3:279–285CrossRefGoogle Scholar
  23. Singh DK, Jagannathan R, Khandelwal P, Abraham PM, Poddar P (2013) In situ synthesis and surface functionalization of gold nanoparticles with curcumin and their antioxidant properties: an experimental and density functional theory investigation. Nanoscale 5:1882–1893CrossRefGoogle Scholar
  24. Sneha K, Sathishkumar M, Lee SY, Bae MA, Yun Y-S (2011) Biosynthesis of Au nanoparticles using cumin seed powder extract. J Nanosci Nanotechnol 11:1811–1814CrossRefGoogle Scholar
  25. Sperling RA, Rivera Gil P, Zhang F, Zanella M, Parak WJ (2008) Biological applications of gold nanoparticles. Chem Soc Rev 37:1896–1908.  https://doi.org/10.1039/b712170a CrossRefGoogle Scholar
  26. Sreelakshmi C, Goel N, Datta K, Addlagatta A, Ummanni R, Reddy B (2013) Green synthesis of curcumin capped gold nanoparticles and evaluation of their cytotoxicity. Nanosci Nanotechnol Lett 5:1258–1265CrossRefGoogle Scholar
  27. Teiten M-H, Eifes S, Dicato M, Diederich M (2010) Curcumin—the paradigm of a multi-target natural compound with applications in cancer prevention and treatment. Toxins 2:128–162CrossRefGoogle Scholar
  28. Yallapu MM, Jaggi M, Chauhan SC (2012) Curcumin nanoformulations: a future nanomedicine for cancer. Drug Discov Today 17:71–80CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of PharmacyUniversity of WaterlooKitchenerCanada
  2. 2.Waterloo Institute for NanotechnologyUniversity of WaterlooWaterlooCanada
  3. 3.Department of Medical PhysicsGrand River Regional Cancer CentreKitchenerCanada
  4. 4.Department of Systems Design EngineeringUniversity of WaterlooWaterlooCanada
  5. 5.Department of Physics and AstronomyUniversity of WaterlooWaterlooCanada
  6. 6.Department of Clinical Studies, Ontario Veterinary CollegeUniversity of GuelphGuelphCanada

Personalised recommendations