Abstract
In the work, different types of zeolites were used for the development of enzyme-based electrochemical biosensors. Zeolites were added to the biorecognition elements of the biosensors and served as additional components of the biomembranes or adsorbents for enzymes. Three types of biosensors (conductometric, amperometric and potentiometric) were studied. The developed biosensors were compared with the similar biosensors without zeolites. The biosensors contained the following enzymes: urease, glucose oxidase, glutamate oxidase, and acetylcholinesterase and were intended for the detection of urea, glucose, glutamate, and acetylcholine, respectively. Construction of the biosensors using the adsorption of enzymes on zeolites has several advantages: simplicity, good reproducibility, quickness, absence of toxic compounds. These benefits are particularly important for the standardization and further mass production of the biosensors. Furthermore, a biosensor for the sucrose determination contained a three-enzyme system (invertase/mutatorase/glucose oxidase), immobilized by a combination of adsorption on silicalite and cross-linking via glutaraldehyde; such combined immobilization demonstrated better results as compared with adsorption or cross-linking separately. The analysis of urea and sucrose concentrations in the real samples was carried out. The results, obtained with biosensors, had high correlation with the results of traditional analytical methods, thus the developed biosensors are promising for practical applications.
Similar content being viewed by others
References
Bonnet C, Andreescu S, Marty J-L (2003) Adsorption: an easy and efficient immobilisation of acetylcholinesterase on screen-printed electrodes. Anal Chim Acta 481:209–211. https://doi.org/10.1016/S0003-2670(03)00122-3
Camblor MA, Corma A, Valencia S (1998) Characterization of nanocrystalline zeolite Beta. Microporous Mesoporous Mater 25:59–74. https://doi.org/10.1016/S1387-1811(98)00172-3
Dai Z, Ju H (2012) Bioanalysis based on nanoporous materials. TrAC Trends Anal Chem 39:149–162. https://doi.org/10.1016/j.trac.2012.05.008
Das P, Das M, Chinnadayyala SR et al (2016) Recent advances on developing 3rd generation enzyme electrode for biosensor applications. Biosens Bioelectron 79:386–397. https://doi.org/10.1016/j.bios.2015.12.055
Hua J, Han Y (2009) One-step preparation of zeolite silicalite-1 microspheres with adjustable macroporosity. Chem Mater 21:2344–2348. https://doi.org/10.1021/cm803366k
Husain Q (2017) Biosensor applications of graphene–nanocomposites bound oxidoreductive and hydrolytic enzymes. Anal Methods 9:6734–6746. https://doi.org/10.1039/C7AY02606D
Kaur B, Srivastava R, Satpati B (2015) Nanocrystalline titanosilicate-acetylcholinesterase electrochemical biosensor for the ultra-trace detection of toxic organophosphate pesticides. ChemElectroChem 2:1164–1173. https://doi.org/10.1002/celc.201500095
Larlus O, Valtchev VP (2004) Crystal morphology control of LTL-type zeolite crystals. Chem Mater 16:3381–3389. https://doi.org/10.1021/cm0498741
Mintova S, Gilson J-P, Valtchev V (2013) Advances in nanosized zeolites. Nanoscale 5:6693. https://doi.org/10.1039/c3nr01629c
Narasimharao K, Hartmann M, Thiel HH, Ernst S (2006) Novel solid basic catalysts by nitridation of zeolite beta at low temperature. Microporous Mesoporous Mater 90:377–383. https://doi.org/10.1016/j.micromeso.2005.11.029
Nenkova R, Wu J, Zhang Y, Godjevargova T (2015) Evaluation of immobilization techniques for the fabrication of nanomaterial-based amperometric glucose biosensors. Anal Lett 48:1297–1310. https://doi.org/10.1080/00032719.2014.979364
Prokešová P, Mintova S, Čejka J, Bein T (2003) Preparation of nanosized micro/mesoporous composites via simultaneous synthesis of Beta/MCM-48 phases. Microporous Mesoporous Mater 64:165–174. https://doi.org/10.1016/S1387-1811(03)00464-5
Rangnekar N, Mittal N, Elyassi B et al (2015) Zeolite membranes—a review and comparison with MOFs. Chem Soc Rev 44:7128–7154. https://doi.org/10.1039/C5CS00292C
Ren L, Dong J, Cheng X et al (2013) Hydrogen peroxide biosensor based on direct electrochemistry of hemoglobin immobilized on gold nanoparticles in a hierarchically porous zeolite. Microchim Acta 180:1333–1340. https://doi.org/10.1007/s00604-013-1064-x
Reverté L, Prieto-Simón B, Campàs M (2016) New advances in electrochemical biosensors for the detection of toxins: nanomaterials, magnetic beads and microfluidics systems. A review. Anal Chim Acta 908:8–21. https://doi.org/10.1016/j.aca.2015.11.050
Rotariu L, Lagarde F, Jaffrezic-Renault N, Bala C (2016) Electrochemical biosensors for fast detection of food contaminants—trends and perspective. TrAC Trends Anal Chem 79:80–87. https://doi.org/10.1016/j.trac.2015.12.017
Scampicchio M, Bulbarello A, Arecchi A et al (2012) Electrospun nonwoven nanofibrous membranes for sensors and biosensors. Electroanalysis 24:719–725. https://doi.org/10.1002/elan.201200005
Teles FRR, Fonseca LP (2008) Applications of polymers for biomolecule immobilization in electrochemical biosensors. Mater Sci Eng C 28:1530–1543. https://doi.org/10.1016/j.msec.2008.04.010
Thévenot DR, Toth K, Durst RA, Wilson GS (2001) Electrochemical biosensors: recommended definitions and classification 1 International Union of Pure and Applied Chemistry: Physical Chemistry Division, Commission I. 7 (Biophysical Chemistry); Analytical Chemistry Division, Commission V. 5 (Electroanalytical). Biosens Bioelectron 16:121–131. https://doi.org/10.1016/S0956-5663(01)00115-4
Tosheva L, Valtchev VP (2005) Nanozeolites: synthesis, crystallization mechanism, and applications. Chem Mater 17:2494–2513. https://doi.org/10.1021/cm047908z
Wang Z-G, Wan L-S, Liu Z-M et al (2009) Enzyme immobilization on electrospun polymer nanofibers: an overview. J Mol Catal B Enzym 56:189–195. https://doi.org/10.1016/j.molcatb.2008.05.005
Willis RR, Benin AI (2007) Toward commercialization of nanozeolites. Stud Surf Sci Catal 170:242–249. https://doi.org/10.1016/S0167-2991(07)80845-7
Acknowledgements
The authors gratefully acknowledge the support of this study by the National Academy of Sciences of Ukraine in the frame of Scientific and Technical Government Program “Sensor systems for medico-ecological and industrial-technological requirement: metrological support and experimental operation”.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Soldatkina, O.V., Kucherenko, I.S., Soldatkin, O.O. et al. Development of electrochemical biosensors with various types of zeolites. Appl Nanosci 9, 737–747 (2019). https://doi.org/10.1007/s13204-018-0725-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13204-018-0725-9