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Applied Nanoscience

, Volume 8, Issue 5, pp 1101–1110 | Cite as

Bio-hybridization of nanobactericides with cellulose films for effective treatment against members of ESKAPE multi-drug-resistant pathogens

  • Syed Baker
  • Tatiana Volova
  • Svetlana V. Prudnikova
  • Anna A. Shumilova
  • Olga V. Perianova
  • Sergey M. Zharkov
  • Andrey Kuzmin
  • Kondratenka Olga
  • Kiryukhin Bogdan
  • Ivan P. Shidlovskiy
  • Zoya K. Potkina
  • Olga Y. Khohlova
  • Tatiana I. Lobova
Original Article

Abstract

The rapid expansion of drug-resistant pathogens has created huge global impact and development of novel antimicrobial leads is one of the top priority studies in the current scenario. The present study aims to develop bio-hybridized nanocellulose films which comprise of phytogenic silver nanobactericides. The nanobactericides were synthesized by treating 1 mM silver nitrate with aqueous extract of Chamerion angustifolium which reduced the metal salt to produce polydispersed nanobactericides which were tested against the members of ESKAPE drug-resistant communities. The synthesized silver nanobactericides were subjected to characterization with UV–visible spectra which displayed maximum absorbance at 408 nm. The bio-molecular interaction of phyto-constituents to mediate synthesis and stabilization of nanobactericides was studied with Fourier-transform infrared spectroscopy (FTIR) which depicted functional groups associated with nanobactericides. The crystalline nature was studied with X-ray diffraction (XRD) which showed Bragg’s intensities at 2θ angle which denoted (111), (200), (220), and (311) planes. The morphological characteristics of silver nanobactericides were defined with transmission electron Microscopy (TEM) image which displayed polydispersity of silver nanobactericides with size ranging from 2 to 40 nm. The synthesized nanobactericides showed a significant activity against MRSA strain with 21 mm zone of inhibition. The minimal inhibitory concentration of silver nanobactericides to inhibit the growth of test pathogens was also determined which ranged between 0.625 and 1.25 μg/ml. The silver nanobactericides were bio-hybridized onto nanocellulose films produced by Komagataeibacter xylinus B-12068 culture strain. The films were dried to determine the mechanical properties which showed increased in Young’s modulus and tensile strength in comparison with control bacterial cellulose films. Overall, the results obtained in the present investigation are promising enough to report bactericidal activity of bio-hybridized nanobactericidal films against ESKAPE. These communities are reported to cause severe threats to all forms of lives irrespective to their habitats which can lead to huge economical crisis.

Keywords

ESKAPE Bio-hybridization Silver nanobactericides Phytogenic Bactericidal activity 

Notes

Acknowledgements

Authors are thankful for Ministry of Education and Science of the Russian Federation for providing funding under the scheme of 5-100: Russian Academic Excellence Project. Authors are grateful for facilities provided by Siberian Federal University to carry out the present study.

Compliance with ethical standards

Conflict of interest

All authors state that they do not have any conflict of interest.

Supplementary material

13204_2018_717_MOESM1_ESM.docx (55 kb)
Supplementary material 1 (DOCX 54 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Syed Baker
    • 1
  • Tatiana Volova
    • 2
    • 3
  • Svetlana V. Prudnikova
    • 4
  • Anna A. Shumilova
    • 3
  • Olga V. Perianova
    • 5
  • Sergey M. Zharkov
    • 3
    • 6
  • Andrey Kuzmin
    • 7
  • Kondratenka Olga
    • 1
  • Kiryukhin Bogdan
    • 1
  • Ivan P. Shidlovskiy
    • 3
  • Zoya K. Potkina
    • 5
  • Olga Y. Khohlova
    • 5
  • Tatiana I. Lobova
    • 5
  1. 1.Laboratory of Biotechnology of New MaterialsSiberian Federal UniversityKrasnoyarskRussia
  2. 2.Institute of Biophysics SB RASFederal Research Center “Krasnoyarsk Science Center SB RAS”KrasnoyarskRussia
  3. 3.Siberian Federal UniversityKrasnoyarskRussia
  4. 4.School of Fundamental Biology and BiotechnologySiberian Federal UniversityKrasnoyarskRussia
  5. 5.Department of MicrobiologyKrasnoyasrk State Medical University named after Prof. VF. Voino-YasenetskiyKrasnoyarskRussia
  6. 6.Kirensky Institute of PhysicsFederal Research Center KSC SB RASKrasnoyarskRussia
  7. 7.School of Petroleum and Natural Gas EngineeringSiberian Federal UniversityKrasnoyarskRussia

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