Synthesis of pH-sensitive poly(β-amino ester)-coated mesoporous silica nanoparticles for the controlled release of drugs
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This report describes the synthesis of a controlled drug delivery system that was obtained by coating mesoporous silica nanoparticles (MSNs) with poly(β-amino ester) (PbAE), which is a solid and stable material at physiological pH, but is dissolved at acidic pH values, such as those in tumor tissues (from 5.0 to 6.5). To synthesize the system, PbAE chains were grafted onto amino-functionalized MSNs through a reaction between the surface amino groups of MSNs and the ends of acrylate chains of a PbAE. The system was physicochemically characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy, transmission electron microscopy, thermogravimetric analysis, X-ray photoelectron spectrometry, and X-ray diffraction analyses. In addition, the in vitro release of doxorubicin (DOX) and doxycycline (DXY) in acidic and physiological media was evaluated. It was observed that the PbAE modification did not affect the mesoporous structure of MSNs. When the amount of 3-aminopropyltriethoxysilane was increased during functionalization, the amount of PbAE binding to MSNs increased as well. With respect to drug release, the sample with the highest amount of PbAE showed better control in the delivery of DXY and DOX in acidic media, because at pH 5.5, the release of both drugs was 40% higher than that at pH 7.4. These results reveal two aspects about the presence of PbAE in MSNs: PbAE does not affect the mesoporous structure of the nanoparticles, and PbAE is the main factor controlling the delivery of drugs in acidic media.
KeywordsMesoporous silica nanoparticles Poly(β-amino ester) pH-sensitive Drug delivery system Doxorubicin
This project was supported by the program of competitive funds within the 2015 FIQ-UADY call. SAXRD and XPS measurements were performed at LANNBIO Cinvestav Mérida, under support from projects FOMIX-Yucatán 2008-108160 and CONACYT LAB-2009-01 No. 123913. MSc. D. Aguilar and Ing. W. Cauich are acknowledged for their technical help.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author states that there are no conflicts of interest.
- Duivenvoorden WC, Popovic SV, Lhotak S, Seidlitz E, Hirte HW, Tozer RG et al (2002) Doxycycline decreases tumor burden in a bone metastasis model of human breast cancer. Cancer Res 62(6):1588–1591Google Scholar
- Huang IP, Sun SP, Cheng SH, Lee CH, Wu CY, Yang CS et al (2011) Enhanced chemotherapy of cancer using pH-sensitive mesoporous silica nanoparticles to antagonize P-glycoprotein-mediated drug resistance. Mol Cancer Therap 10(5):761–769. https://doi.org/10.1158/1535-7163.mct-10-0884 CrossRefGoogle Scholar
- Jakša G, Štefane B, Kovač J (2013) XPS and AFM characterization of aminosilanes with different numbers of bonding sites on a silicon wafer. Surf Interface Anal 45(11–12):1709–1713Google Scholar
- Kim Y, Zhang C, Cho C, Cho M, Jiang H (2013) Poly (amino ester)s-based polymeric gene carriers in cancer gene therapy. In: Wei M, Good D (eds) Novel gene therapy approaches. https://doi.org/10.5772/54740
- Na K (2007) pH-sensitive polymeric micelles for the effective delivery of anti-cancer drug. Korean J Gastroenterol Taehan Sohwagi Hakhoe Chi 49(5):314–319Google Scholar
- Naruphontjirakul P, Viravaidya-Pasuwat K (2011) Development of doxorubicin—core–shell chitosan nanoparticles to treat cancer. In: Paper presented at the proceedings of the international conference on biomedical engineering and technology, IACSIT Press, Singapore, vol 11, pp 90–94Google Scholar
- Petrescu M, Mitran RA, Luchian AM, Matei C, Berger D (2015) Mesoporous ceria-silica composites as carriers for doxycycline. UPB Sci Bull Ser B: Chem Mater Sci 77(3):13–24Google Scholar
- Stuart BH (2004) Organic molecules. I: David JA, Dartford K (ed), Infrared spectroscopy: Fundamentals and applications (pp. 71–80, 83). John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England, WileyGoogle Scholar
- Yang K, Zhang C, Wang W, Wang PC, Zhou J, Liang X (2014) pH-responsive mesoporous silica nanoparticles employed in controlled drug delivery systems for cancer treatment. Cancer Biol Med 11(1):34Google Scholar