Water source quality testing in Gezira State, Sudan, using the compartment bag test


Although poor water quality is recognized as a public health threat, it has been little investigated in Sudan. In this paper, water sources in Gezira State, Greater Wad Medani locality, have been categorized as safe, intermediate safe and high-risk unsafe sources using the compartment bag test (CBT) to detect E. coli, which is an indicator of fecal contamination of water. The CBT is simple, portable and self-contained, and it can be done in the field environment. A total of 122 samples were collected from different water sources and included rivers, water treatment plant, boreholes/tube wells, hand pumps, public water taps, public water coolers, public elevated water tanks and household elevated water tanks. It was found that 69% (84/122) of investigated water sources were safe to drink. The sources most likely to be contaminated were those close to industrial points and factories or open sources exposed to pollution. The result showed that the highest level of contamination of water sources (high risk and unsafe) was observed in rural area (9.1%) followed by urban (5.7%) and peri-urban (1.6%). Frequent and routine qualitative analysis of water sources using CBT is recommended to improve human health and hence the country’s development.


Drinking water in developing countries is irregularly tested and monitored for quality because it is extremely difficult and costly to monitor water quality in low-resource settings. Globally, water quality is recognized as one of the greatest threats to human health (Cairncross 2013). UNICEF and WHO reported that over 780 million people are still lacking access to safe sources of drinking water (UNICEF/WHO 2012). For several decades, about a billion people in developing countries have not had a safe water supply (Hunter et al. 2010).

Contaminated drinking water, along with inadequate supplies of water for personal hygiene and poor sanitation, are the main contributors to an estimated 4 billion cases of diarrhea each year causing 2.2 million deaths, mostly among children under the age of five (Clasen and Bastable 2003). An estimated 884 million people are drawing their drinking water from lakes and rivers, unprotected wells and springs, and other sources that are often highly contaminated with waterborne pathogens (Rosa and Clasen 2010). In sub-Saharan Africa, 42% of the population is without improved water, 64% is without improved sanitation, and deaths due to diarrheal diseases are greater than in any other region (Montgomery and Elimelech 2007). Barbieri et al. (2019) assessed the groundwater quality in the buffer zone of Limpopo National Park and found that only 13.3% of the groundwater was fresh and suitable for drinking purposes; the remaining 86.6% was brackish and undrinkable.

Unsafe water and poor sanitation and hygiene have been reported to rank third among the 20 leading risk factors for health burden in developing countries, including Sudan (Shanan et al. 2015). In Sudan where adequate quantities of domestic water are already available on demand, the main task over the next few years will be to sustain water quality. Eltigani et al. (2015) concluded that greater qualitative efforts are needed to make water sources safe to use. Although Sudan is a country particularly rich in water resources, the quality and safety of its drinking water sources have not been fully investigated and are therefore largely unknown.

It has been reported that access to water and sanitation are extremely low in rural areas in Sudan (USAID 2009). In Gezira, more than 70% of populations in the villages are infected by waterborne diseases mainly because of the use of the polluted irrigation canal water for their domestic water needs (Henri et al. 2002).

The surface water quality is a matter of serious concern today (Singh et al. 2005). Water sources used in Sudan are diverse. The Blue Nile and groundwater are the most important water sources in Sudan and Gezira State particularly. Other sources are directly or indirectly linked with rivers and groundwater. These include water taps, elevated tanks, public water pot taps, boreholes, irrigation canals and hand pumps. The risk posed by each of these sources varies greatly. Approximately 13.5% prevalence of diarrheal diseases in Sudan was recorded among children living in areas where people draw water from uncertified Hafirs (Shanan et al. 2015). Both sources present health hazards to the users. Sudan has six irrigation schemes. Expansion of irrigation systems has adverse impacts on quality of water resources (Valipour 2014). Murcott, et al. (2015) stated that in both humanitarian emergency and development settings, bacteriological water quality testing is essential as it provides vital information on the microbial safety of drinking water resources. Zamxaka et al. (2004) confirmed that the evaluation of potable water supplies for coliform bacteria is important in determining the sanitary quality of drinking water.

Little work has been done on microbial quality of the drinking water in Sudan. Musa et al. (1999) investigated water quality in northern Sudan and found that fecal coliform counts grossly in excess of WHO standards. Ell-Amin et al. (2010) have carried out microbiological tests on the drinking water quality of Wad Medani and found that both surface and groundwater were highly contaminated with total coliform and fecal coliform. In addition, El Karim et al. (1985) conducted a study in Sudan and found that all water sources were invariably contaminated with coliforms. Abdelrahman and Eltahir (2011) found that in South Darfur (Sudan) the highest level of contamination of water sources was observed in household storage containers (20%) followed by boreholes (11.25%), reservoirs (6.24%), hand pumps (5.42%) and dug wells (2.49%). Generally, these water sources have been used without any routine qualitative analysis.

Escherichia coli are present because of breaks in water lines, uncovered sources and mismanagement of sources. The present study focused on E. coli in water sources using the CBT. Categorization of water sources using WHO risk guidelines to safe and unsafe water will help pinpoint risky sources and hence assist in improving community health.

Materials and methods

Description of study area

Gezira State is located in the central part of Sudan. It is bounded by latitudes 14°24′04″N and 33°31′11″E (Kogan 1990) (Fig. 1). The state is considered to be one of the most densely populated with a total population of 3.5 million people (53% rural, 35% urban and 12% nomad) with an annual growth rate of 2.9% (Sudan population census 2008, 2010; Gezira Population Statistical Office). Rainfall in Gezira ranges from 250 to 350 mm, and the soil is predominantly clay (Elias et al. 2001). Most of the rural populations are agro-pastoralists, according to the 5th Sudan population and housing census held in 2008. The state is surrounded by the White Nile and Dinder Rivers and traversed by the Blue Nile and Rahad Rivers. The state is divided into seven localities (administrative subdivisions). This study was conducted at Greater Wad Medani locality which included urban, peri-urban and rural communities. Wad Medani city is the capital of the state. The mean monthly temperature in the state is about 22 °C in January and 34 °C in May. Humidity varies from 13% in the north to more than 60% in the southern part of the state.

Fig. 1

Gezira State, Sudan (Eltigani et al. 2015)

Sample collection

Samples were collected to represent the whole study area. Samples were collected during June and July 2016 where temperature ranged from 40.3 to 25.5 °C maximum and minimum, respectively. A total number of 122 samples were collected from different sources and included river, water treatment plant, boreholes, hand pumps, public water taps, public water pots, public water coolers, public water tanks and household water tanks. Table 1 provides a basic description of all water sources assessed in this study.

Table 1 Water sources characteristics

Physical parameters of water

Temperature, pH and turbidity were measured for each sample collected. Temperature was measured using a thermometer (76-mm immersion, made in UK). Temperature will have an impact on the acceptability of a number of other inorganic constituents and chemical contaminants that may affect taste. High water temperature enhances the growth of microorganisms and may increase problems related to taste, odor, color and corrosion.

A pH meter (Sension 3 Millimeter, HACH) was used to measure pH value in all samples collected. High pH causes a bitter taste; biological contamination can change a waters pH, which in turn can harm animals and plants living in the water (Patil et al. 2012).

Turbidity was measured in nephelometric turbidity units (NTU). According to the WHO guidelines the 5th edition, turbidity must be at the level up to 5 NTU (WHO 2012). Turbidity consists of suspended material in water, causing a cloudy appearance. The suspended matter may be inorganic or organic. Turbidity in surface waters may be the result of particulate matter of many types and is more likely to include attached microorganisms that are a threat to health (WHO 2011).

Water sample assessment using the compartment bag test

CBT is simple, portable and self-contained, and it can be done in a field environment. For each water sample, the supplied E. coli chromogenic medium was dissolved in 100 mL of the water sample after which the sample was poured into the specially designed 100-mL compartment bag and incubated at 37 °C for 24 h in an incubator (MIR-153, SANIO). Each compartment bag is divided into 1-mL, 3-mL, 10-mL, 30-mL and 56-mL compartments (Fig. 2). Distilled sterile water was used for negative control and water containing E. coli as positive control. CBT calculated the most probable number (MPN) of E. coli per each 100 mL of water. Results were registered using WHO standards (Table 2).

Fig. 2

Compartment bag (Stauber et al. 2014)

Table 2 Health risk based on WHO guidelines for drinking water quality (WHO 2011)

Statistical analysis

All water assessment data were entered into a Microsoft Excel spreadsheet. After cleaning of the data, it was exported to Stata 14 statistical package for data analysis. Percentages, frequencies and cross tables were used to discuss the results.

Results and discussion

Physical parameters of water sources

In this study, the parameters included pH, turbidity and temperature that may indicate changes in water quality. A pH meter was used to measure pH of collected samples in the field. The results showed a mean range from 7.4 to 7.8 for all sources (Table 3), which indicated that the water sources were neutral.

Table 3 Physical parameters of sources

Turbidity is a standard measurement in water sampling where suspended sediment plays an important role and may be useful for estimating E. coli in water particularly for lake water and reservoirs. In Sudan, turbidity of waters can be caused by air molecules where the air is dusty. Turbidity was observed to be 5500 NTU and 45.4 NTU for river and irrigation canal water, respectively (Table 3). These high measurements may indicate the presence of pathogenic microorganisms in these water sources.

Temperature is reported to influence the rate of chemical reactions. The mean values for temperatures ranged from 23.6 to 28.2 °C for all samples collected. The temperature of cooler containers was seen as 23.6 °C. High temperature of 28.2 °C in bottled water can be explained by the fact that the data were taken at midday when temperatures are usually very high in Sudan.

Water quality of sources

The results showed that 69% of samples collected (84/122) were safe, 14% intermediate (17/122), 6% in high risk (7/122), and 12% unsafe (14/122) water (Table 4). Water sources contained E. coli bacteria with variable degrees. River and irrigation canals water were completely unsafe for consumption. The reason might be that these sources are open and exposed to pollutants. Zamxaka et al. (2004) found that more turbid sources are the most microbiologically contaminated. The results showed an association between high turbidity in rivers and irrigation canals and the presence of E. coli. Water quality might vary rapidly, due to the rainfall that can greatly increase the levels of microbial contamination in open sources. Hunter (2003) found that waterborne outbreaks often occur following rainfall.

Table 4 Water sources with WHO water quality risk categories

This result is in line with Valipour (2014) who stated that the increase in irrigation systems has adverse impacts on quality of water resources. Some farmer communities living inside the Gezira irrigation scheme area used canal water for drinking and washing. Boelee et al. (2007) confirmed that in irrigation systems all over the world, water is not only used for the irrigation of agricultural crops, but for a whole range of domestic and other purposes as well. Most of the industrial cities (Wad Medani industrial area) and sugar factories around the study area (Sennar sugar factory) discharge their waste into the river which could have serious adverse health effects on the communities using water for domestic purposes.

A total of 65% of household water taps, 75% of yard taps and 59% of communal taps were safe. Taps normally takes water either from treatment plants through the network distribution of boreholes. The water treatment plant was found to be 100% E. coli free, and 82% of the boreholes contained safe water. Boreholes indicated safe groundwater. This result did not agree with results published by Ell-Amin et al. (2010) which indicated that Wad Medani groundwater was highly contaminated. This research was conducted in the Wad Medani locality, while Amira’s work included the Managil locality which is known for polluted water. A study carried out by Engström et al. (2015) in Juba indicated that an important contamination mechanism was fecal pollution of the contributing groundwater, which was probably due to the presence of latrines. Groundwater aquifers in these areas are not rich and communities depend mainly on surface water, which is liable to contamination. Elevated tanks are found either in households or in public schools and are well controlled and routinely checked, and it could therefore account that 64% tanks were containing safe water (Table 4).

Risk category according to urban, peri-urban and rural areas

In this study, urban population are those living within the Wad Medani city, while the peri-urban communities are those living around the city and rural communities are living in villages away from the city where most of them practice agriculture. Table 5 shows the quality of water sources in urban, peri-urban and rural areas of the study area.

Table 5 Location with water sources with WHO water quality risk categories

Generally, water sources in urban area are safe particularly treatment plant and bottled water (vendor water) (Table 5). There was, however, tap water which was classified as unsafe for drinking water in the urban area. The river water was totally unfit for drinking water as was one water cooler. Boreholes were found in all areas and generally safe except for 3 boreholes in the rural communities. Water tanks in rural areas were 54.5% safe. Canal water is extended through peri-urban and rural areas. The majority of the rural people are cultivators (Musa et al. 1999) with fields irrigated by water drawn from the canals which is unsafe. The canal water was 33.3% high risk in peri-urban and 66.7% unsafe in rural area. The result shows that the highest level of contamination of water sources (high risk and unsafe) was observed in rural area (9.1%) followed by urban (5.7%), and peri-urban (1.6%).


Quality of water sources is highly affected by industrial and factory wastes coming from upstream Wad Medani. The most contaminated sources are those that are open, turbid and exposed to pollution. Overall the CBT gave fast results and provided a general indication of the water quality in the study area. Frequent and routine qualitative analysis of water sources using the CBT is recommended in Sudan to improve human health and development in areas like Wad Medani with little or no resources. Shift to integrated water resources management approach, where water quality is highly considered, will lead to better and sustainable protection of water sources.


  1. Abdelrahman AA, Eltahir YM (2011) Bacteriological quality of drinking water in Nyala, South Darfur, Sudan. Environ Monit Assess 175(1):37–43

    Article  Google Scholar 

  2. Barbieri M, Ricolfi L, Vitale S, Muteto PV, Nigro A, Sappa G (2019) Assessment of groundwater quality in the buffer zone of Limpopo National Park, Gaza Province, Southern Mozambique. Environ Sci Pollut Res 26(1):62–77

    Article  Google Scholar 

  3. Bashier EE, Bashir NH, Mohamadani A, Elamin SO, Abdelrahman SH (2015) A challenge of sustaining water supply and sanitation under growing population: a case of the Gezira state. Int J Water Resour Environ Eng, Sudan. https://doi.org/10.5897/IJWREE2015

    Book  Google Scholar 

  4. Boelee E, Laamrani H, Van der Hoek W (2007) Multiple use of irrigation water for improved health in dry regions of Africa and South Asia. Irrig Drain 56(1):43–51

    Article  Google Scholar 

  5. Cairncross S (2013) Sanitation in the developing world: current status and future solutions. Int J Environ Health Res 13(S):S123–S131

    Google Scholar 

  6. Clasen TF, Bastable A (2003) Faecal contamination of drinking water during collection and household storage: the need to extend protection to the point of use. J Water Health 1(3):109–115

    Article  Google Scholar 

  7. El Karim MA, El Hassan BM, Hussein KK (1985) Social and public health implication of water supply in arid zones in the Sudan. Social Sci Med 20(4):393–398

    Article  Google Scholar 

  8. Elias E, Salih A, Alaily F (2001) Cracking patterns in the virisols of the Sudan, Gezira at the end of dry season. J Int Agro Phys 15(3):151–155

    Google Scholar 

  9. Ell-Amin AM, Sulieman AME, El-Khalifa EA (2010) Quality characteristics of drinking water in Khartoum state and Wad-Medani District, Sudan. In: 14th international water technology conference, IWTC 14 2010, Cairo, Egypt. pp 863–876

  10. Engström E, Balfors B, Mörtberg U, Thunvik R, Gaily T, Mangold M (2015) Prevalence of microbiological contaminants in groundwater sources and risk factor assessment in Juba, South Sudan. Sci Total Environ 515:181–187

    Article  Google Scholar 

  11. Henri S, Chevillard C, Mirgani A, Paris P, Gaudart J, Camilla C, Dessien H, Montero F, Elwali NM, Saeed OK, Magzoub M, Dessien AJ (2002) Cytokine regulation of periportal fibrosis in humans infected with Schistosoma mansoni. J Immunol 169(2):929–936

    Article  Google Scholar 

  12. Hunter PR (2003) Climate change and waterborne and vector-borne disease. J Appl Microbiol 94(s1):37–46

    Article  Google Scholar 

  13. Hunter PR, MacDonald AM, Carter RC (2010) Water supply and health. PLoS Med 7(11):1350

    Article  Google Scholar 

  14. Kogan FN (1990) Remote sensing of weather impacts on vegetation in non-homogeneous areas. Int J Remote Sens 11(8):1405–1419

    Article  Google Scholar 

  15. Montgomery MA, Elimelech M (2007) Water and sanitation in developing countries: including health in the equation. Environ Sci Technol 41(1):17–24

    Article  Google Scholar 

  16. Murcott S, Keegan M, Hanson A, Jain A, Knutson J, Liu S, Wong TK (2015) Evaluation of microbial water quality tests for humanitarian emergency and development settings. Procedia Eng 107:237–246

    Article  Google Scholar 

  17. Musa HA, Shears P, Kafi S, Elsabag SK (1999) Water quality and public health in northern Sudan: a study of rural and peri-urban communities. J Appl Microbiol 87(5):676–682

    Article  Google Scholar 

  18. Patil PN, Sawant DV, Deshmukh RN (2012) Physico-chemical parameters for testing of water-a review. Int J Environ Sci 3(3):1194

    Google Scholar 

  19. Rosa G, Clasen T (2010) Estimating the scope of household water treatment in low-and medium-income countries. Am J Trop Med Hygiene 82(2):289–300

    Article  Google Scholar 

  20. Shanan S, Abd H, Bayoumi M, Saeed A, Sandström G (2015) Prevalence of protozoa species in drinking and environmental water sources in Sudan. BioMed Int 2015:345619

    Google Scholar 

  21. Singh KP, Malik A, Sinha S (2005) Water quality assessment and apportionment of pollution sources of Gomti river (India) using multivariate statistical techniques—a case study. Anal Chim Acta 538(1):355–374

    Article  Google Scholar 

  22. Stauber C, Miller C, Cantrell B, Kroell K (2014) Evaluation of the compartment bag test for the detection of Escherichia coli in water. J Microbiol Methods 99:66–70

    Article  Google Scholar 

  23. UNICEF WHO (2012) Progress report on drinking water and sanitation. http://www.unicef.org/media/files/JMPreport2012.pdf

  24. USAID (2009) Sudan water and sanitation profile. http://www.usaid.gov/pdf.docs/PNADO924.pdf

  25. Valipour M (2014) Drainage, water logging, and salinity. Arch Agron Soil Sci 60(12):1625–1640

    Article  Google Scholar 

  26. WHO (2011) Guidelines for drinking-water quality. WHO Chron 38:104–108

    Google Scholar 

  27. WHO (2012) Progress on drinking-water and sanitation—2012 update. Launched on 6 March 2012

  28. Zamxaka M, Pironcheva G, Muyima N (2004) Microbiological and physico-chemical assessment of the quality of domestic water sources in selected rural communities of the Eastern Cape Province, South Africa. Water SA 30(3):333–340

    Google Scholar 

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The authors would like to acknowledge funding from WELLCOME TRUST to the Scientist Network for Outcomes from Water and Sanitation (SNOWS) and University of Venda (Project I493). The effort of University of Gezira in completion of this research is highly appreciated. The authors are grateful to Gezira Water Corporation for providing some logistics during field work. The authors would also like to acknowledge all those who helped and facilitated the field work in Sudan.

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Abdelgalili, E.B., Ahmed, M., Adam, J. et al. Water source quality testing in Gezira State, Sudan, using the compartment bag test. Appl Water Sci 9, 193 (2019). https://doi.org/10.1007/s13201-019-1079-5

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  • Compartment bag test
  • Gezira State
  • Sudan
  • Water sources quality