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The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth

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Abstract

This study focused on the genetic, symbiotic and phenotypic diversity of a collection of 12 Vachellia seyal and Prosopis juliflora rhizobial strains that were isolated from soils influenced by the roots of the grass Sporobolus robustus. Phylogenetic trees were constructed based on data from the 16S rRNA gene, from a multi-locus sequence analysis (MLSA) of the housekeeping genes recA, atpD, glnA and gyrB, as well as from the symbiotic genes nodA, nodC and nifH. Most of the mesorhizobial strains were clustered with the M. plurifarium type strain ORS 1032T and the 16S rRNA gene was as discriminatory as the other housekeeping genes. The Ensifer collection was closely related to E. alkalisoli YIC 4027T and E. fredii LMG 6217T using 16S rRNA and MLSA. Amplification of the Rhizobium collection was only observed with the 16S rRNA gene, where the strains were clustered with Rhizobium sp. 1B, Rhizobium sp. SEMIA 6411, Rhizobium sp. AS1-101a and Rhizobium sp. ORS 3441. The phylogeny of the nifH gene from Mesorhizobium and Ensifer strains were similar to those of nodulation genes (nodA and nodC). The nodA-nodC and nifH gene phylogenies were not consistent with those of 16S rRNA and the housekeeping genes. Ensifer genospecies tolerated high salinity better than the other genotypes after testing on a salt content gradient up to 49‰. We conclude that the rhizosphere of Sporobolus tussocks harbors a rhizobial microbiota, which could be capable of helping V. seyal and P. juliflora to grow better in saline soils.

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Acknowledgments

We thank Joël Fardoux (LSTM, Montpellier) for his valuable technical assistance. This work was funded by the International Foundation for Science (IFS). The authors wish to thank Peter Biggins and the Editor-in-Chief of Symbiosis for reviewing the English of the manuscript.

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Authors and Affiliations

  1. Laboratoire Commun de Microbiologie (LCM) IRD/ISRA/UCAD, Centre de Recherche de Bel-Air, Dakar, Senegal

    Fatoumata Fall, Niokhor Bakhoum, Mathieu Ndigue Faye & Diegane Diouf

  2. Laboratoire Mixte International Adaptation des Plantes et microorganismes associés aux Stress Environnementaux (LAPSE), Dakar, Senegal

    Fatoumata Fall, Niokhor Bakhoum, Mathieu Ndigue Faye & Diegane Diouf

  3. CIRAD, UMR LSTM, F-34398, Montpellier, France

    Christine Le Roux

  4. LSTM, Univ Montpellier, CIRAD, IRD, Montpellier SupAgro, Montpellier, France

    Christine Le Roux & Amadou Mustapha Bâ

  5. Laboratoire de Biologie et Physiologie Végétales, Université des Antilles, Guadeloupe, France

    Amadou Mustapha Bâ

  6. Centre National de Recherches Agronomiques (CNRA), Bambey, Senegal

    Dioumacor Fall

  7. Université du Sine Saloum El Hadj Ibrahima Niass (USSEIN), Environnement, Biodiversité et Développement Durable, Kaolack, Senegal

    Niokhor Bakhoum & Diegane Diouf

  8. UMR195 Lemar, Dakar, Senegal

    Oumar Sadio

  9. Département de Biologie Végétale, Université Cheikh Anta Diop de Dakar, Dakar, Senegal

    Oumar Sadio

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  1. Fatoumata Fall
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Correspondence to Fatoumata Fall.

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Fall, F., Le Roux, C., Bâ, A.M. et al. The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth. Symbiosis 84, 61–69 (2021). https://doi.org/10.1007/s13199-021-00763-7

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