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Lessons from culturing lichen soredia

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Abstract

Vegetative propagules play various important roles in lichen biology. We cultured soredia of Cladonia lichens in vitro and obtained three noteworthy results. Firstly, soredia are a beneficial source for the isolation of lichen symbionts. The mycobiont was obtained from 66% and the photobiont from 67% of the cultured soredia that were not contaminated. Secondly, the development of soredia followed a previously recognized pattern, arachnoid stage – soredium field – primordium, but a thalline structure was not achieved. We suggest that thallus formation in vitro is a question of favourable environmental factors, not partners compatibility. Finally, we discovered that fungi, other than the mycobiont, as well as airborne contaminants are dispersed together with lichen soredia. This is the first-ever report of such a phenomenon. The possible ecological consequences are discussed. Cystobasidiomycete yeasts were found among these fungi. We isolated representatives of three different lineages from a single thallus suggesting a low specificity for this association.

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Acknowledgements

This study was funded by the Primus Research Programme of Charles University SCI/13. IČ was supported by the STARS programme of the Faculty of Science, Charles University, Prague.

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    Ivana Černajová & Pavel Škaloud

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Černajová, I., Škaloud, P. Lessons from culturing lichen soredia. Symbiosis 82, 109–122 (2020). https://doi.org/10.1007/s13199-020-00718-4

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