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Proteomic evaluation of the freshly isolated cyanobionts from Azolla microphylla exposed to salinity stress

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Abstract

The cyanobiont Anabaena azollae performs nitrogen fixation in the agronomically important Azolla-Anabaena system. However, the response of the cyanobiont to salinity has not been studied at the molecular level. In the present study Azolla microphylla plants were exposed to salinity (90 mM NaCl) for 3 days and the cyanobionts have been isolated. The proteome profile of the freshly isolated cyanobionts were investigated by Two dimensional gel electrophoresis (2DE) followed by MALDI-TOF-MS/MS analysis. Sixty eight protein spots showed significant changes in response to salinity treatment and out of those proteins, 18 protein spots were identified. Among them 12 proteins were up regulated whereas 6 proteins were down regulated. Up regulation of important proteins related to protein synthesis and cell signaling may be involved in the response of the cyanobiont to adapt to short term salinity exposure. The study might help in understanding the biological processes and stress proteins involved in salinity stress adaptation in the cyanobiont A. azollae.

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Acknowledgements

We are grateful to ICAR-Indian Agricultural Research Institute and Indian Council for Agricultural Research, New Delhi for facilities. Financial assistance from SERB, Department of Science and Technology, New Delhi (SR/SO/PS/0077/2010) and HRDG, Council of Scientific and Industrial Research, New Delhi (No. 8(1319)/12/EMR-II) is gratefully acknowledged. Thanks are also due to Prof. N.K. Singh, ICAR-National Research Centre on Plant Biotechnology, New Delhi for MALDI-TOF-MS/MS analysis.

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Authors and Affiliations

  1. Department of Biological Sciences, Sam Higginbottom University of Agriculture Technology and Sciences, Allahabad, UP, 211007, India

    Ravindra Kumar Yadav & Pramod Wasudeo Ramteke

  2. Centre for Conservation and Utilization of BGA, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Keshawanand Tripathi & Gerard Abraham

  3. ICAR-National Research Centre on Plant Biotechnology, Pusa Campus, New Delhi, 110012, India

    Vagish Mishra

  4. Department of Botany, Banaras Hindu University, Varanasi, UP, 221005, India

    Pawan Kumar Singh

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  1. Ravindra Kumar Yadav
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Contributions

GA, PKS and PWR planned the experiments and provided intellectual inputs for the work. RKY conducted the experiments, KNT assisted in recording the observations and VM helped in the MALDI-TOF-MS/MS analysis.

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Correspondence to Gerard Abraham.

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Yadav, R.K., Tripathi, K., Mishra, V. et al. Proteomic evaluation of the freshly isolated cyanobionts from Azolla microphylla exposed to salinity stress. Symbiosis 77, 249–256 (2019). https://doi.org/10.1007/s13199-018-0586-8

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