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Effect of Pinus ponderosa afforestation on soilborne Frankia and saprophytic Actinobacteria in Northwest Patagonia, Argentina

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Abstract

Large areas in the extra-Andean region in the forest - steppe ecotone in “Northwestern Argentinean Patagonia” have been replaced by plantations of the exotic conifer Pinus ponderosa which modify soils physical and chemical factors and alter the biodiversity. Considering that in the region occur saprophytic soilborne actinobacteria that play important role as the fixation of atmospheric nitrogen (N2) in symbiosis with native plant species and the production of bioactive molecules in plants rhizosphere, we aimed to study the effect of the plantation on the abundance of the N2 fixer Frankia and on the genus diversity of cultivable rhizospheric actinobacteria. The study was performed with soils of six paired sites with pine plantations and natural neighbor areas (including steppes or shrublands). Abundance of infective Frankia was estimated by evaluating the nodulation capacity of soils, through a plant bioassay using Ochetophila trinervis as trap plant. Isolation trials for saprophytic actinobacteria were performed by applying chemotactic and successive soils dilutions methods. We concluded that P. ponderosa afforestation affect soil actinobacteria. This was mainly evidenced by a decrease in the Frankia nodulation capacity in O. trinervis, which was related to plantation age, to lower soil carbon and nitrogen content, higher available phosphorus, and to a slight decrease in soils pH. Pine plantation influence on the cultivable saprophytic actinobacteria was less clear. The study highlights the importance of soils as source of Frankia and rhizospheric actinobacteria in relation to disturbance caused by pine plantation in natural environments with native actinorhizal plant species.

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Acknowledgments

We thank two anonymous referees that contributed to improve the quality of the paper. Financial support for this study was provided through grants by Universidad Nacional del Comahue. M. Solans and C. Mestre are members of CONICET.

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Authors and Affiliations

  1. Centro Regional Universitario Bariloche, Universidad Nacional del Comahue, Quintral 1250, R8400FRF, S.C. Bariloche, Argentina

    Natalia B. Arancibia, Mariana Solans, M. Cecilia Mestre & Eugenia E. Chaia

  2. INIBIOMA / CONICET, San Carlos de Bariloche, Argentina

    Mariana Solans

  3. IPATEC / CONICET, San Carlos de Bariloche, Argentina

    M. Cecilia Mestre

  4. INIBIOMA, San Carlos de Bariloche, Argentina

    Eugenia E. Chaia

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  1. Natalia B. Arancibia
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  2. Mariana Solans
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  3. M. Cecilia Mestre
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  4. Eugenia E. Chaia
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Correspondence to Eugenia E. Chaia.

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Arancibia, N.B., Solans, M., Mestre, M.C. et al. Effect of Pinus ponderosa afforestation on soilborne Frankia and saprophytic Actinobacteria in Northwest Patagonia, Argentina. Symbiosis 76, 129–137 (2018). https://doi.org/10.1007/s13199-018-0538-3

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