Symbiosis

, Volume 73, Issue 1, pp 7–14 | Cite as

Nodulation ability in different genotypes of Phaseolus lunatus by rhizobia from California agricultural soils

  • Ademir Sérgio Ferreira de Araujo
  • Angela Celis de Almeida Lopes
  • Jorge C. Berny Mier y Teran
  • Antonia Palkovic
  • Paul Gepts
Article

Abstract

Phaseolus lunatus is the second economically most important species of the genus Phaseolus. It carries out N fixation through symbiosis with rhizobia. However, it is unclear whether P. lunatus can nodulate with native rhizobia from soils where this legume is not native or was not cultivated previously. Thus, this study assessed the ability of 14 geographically distant lima bean genotypes to nodulate with rhizobia from three California agricultural soils: without a history of legumes or P. lunatus cultivation, with a history of legumes as a cover crop, and with a history of P. lunatus cultivation. Nodulation only occurred on genotypes grown in the soil with a history of P. lunatus planting. The analysis of variance of nodulation traits showed that the genotype effect was highly significant in all the traits measured. Shoot biomass had a higher correlation with nodule size and nodule weight than with nodule number. In addition, shoot biomass and leaf N content were positively correlated with nodule coloration and with nodule position close to the main root of the plant. This study suggests that agricultural soils from California do not appear to have native rhizobia able to nodulate P. lunatus, which suggests the need to inoculate, at least initially, the seeds at planting in order to establish the population of rhizobia. Also, geographically distant lima bean genotypes have different responses to nodulating bacteria and it suggests that future studies to test these genotypes across different environments should be pursued.

Keywords

Lima bean N fixation Legumes Bradyrhizobium 

References

  1. Allen ON, Allen EK (1939) Root nodule bacteria of some tropical leguminous plants. II. Cross nodulation test with cowpea group. Soil Sci 47:63–76CrossRefGoogle Scholar
  2. Andueza-Noh RH, Martha L, Serrano-Serrano MI, Chacón Sánchez I, Sanchéz del Pino L, Camacho-Pérez J, Coello-Coello J, Cortes M, Debouck DG, Martínez-Castillo J (2013) Multiple domestications of the Mesoamerican gene pool of lima bean (Phaseolus lunatus L.): evidence from chloroplast DNA sequences. Gen Res Crop Evol 60:1069–1086CrossRefGoogle Scholar
  3. Ballhorn DJ, Schädler M, Elias JD, Millar JA, Kautz S (2016) Friend or foe - light availability determines the relationship between mycorrhizal fungi, rhizobia and lima bean (Phaseolus lunatus L.). PLoS One 11:e0154116CrossRefPubMedPubMedCentralGoogle Scholar
  4. Brahmaprakash GP, Sahu PK (2012) A review: biofertilizers for sustainability. J Indian Instit Sci 92:37–62Google Scholar
  5. Cheminingwa GN, Vessey JK (2006) The abundance and efficacy of Rhizobium leguminosarum bv. viciae in cultivated soils of the eastern Canadian prairie. Soil Biol Biochem 38:294–302CrossRefGoogle Scholar
  6. R Core Team (2016) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
  7. Costa Neto VP (2016) Nodulação e fixação biológica de nitrogênio em feijão-fava inoculado com rizóbios isolados de solos da microrregião do Médio Parnaíba Piauiense. Dissertação Mestrado em Agronomia. Universidade Federal do Piauí, Teresina, PI, Brazil, p 59Google Scholar
  8. Durán D, Rey L, Mayo J, Zúñiga-Dávila D, Imperial J, Ruiz-Argüeso T, Martínez-Romero E, Ormeño-Orrillo E (2014) Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of lima bean (Phaseolus lunatus L.) in Peru. Int J Syst Evol Microbiol 64:2072–2078CrossRefPubMedGoogle Scholar
  9. Fofana B, Vekemans X, Jardin du P, Baudoin JP (1997) Genetic diversity in lima (Phaseolus lunatus L.) as revealed by RAPD markers. Euphytica 95:157–165CrossRefGoogle Scholar
  10. Golluscio R, Faigón A, Tanke M (2006) Spatial distribution of roots and nodules, and δ15N evidence of nitrogen fixation in Adesmia volckmanni, a Patagonian leguminous shrub. J Arid Environ 67:328–335CrossRefGoogle Scholar
  11. Gutiérrez Salgado A, Gepts P, Debouck DG (1995) Evidence for two gene pools of the lima bean, Phaseolus lunatus L., in the Americas. Gen Res Crop Evol 42:15–28CrossRefGoogle Scholar
  12. Howieson JG, Yates RJ, O'Hara GW, Ryder M, Real D (2005) The interactions of Rhizobium leguminosarum biovar trifolii in nodulation of annual and perennial Trifolium spp. from diverse centres of origin. Aust J Exp Agric 45:199–207CrossRefGoogle Scholar
  13. Hungria M, Boher TRJ (2000) Variability of nodulation and dinitrogen fixation capacity among soybean cultivars. Biol Fertil Soil 31:45–52CrossRefGoogle Scholar
  14. Ikeda J (1999) Differences in numbers of nodules and lateral roots between soybean (Glycine max L. Merr.) cultivars, kitamusume and toyosuzu. Soil Sci Plant Nutr 45:591–598CrossRefGoogle Scholar
  15. Keeney DR, Nelson DW (1982) Nitrogen-inorganic forms. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, 2nd edn. American Society of Agronomy, Madison, pp. 643–698Google Scholar
  16. Lobell DB, Gourdji M (2012) The influence of climate change on global crop productivity. Plant Physiol 160:1686–1697CrossRefPubMedPubMedCentralGoogle Scholar
  17. López-López A, Negrete-Yankelevich S, Rogel MA, Ormeño-Orrillo E, Martínez Esperanza J, Martínez-Romero E (2013) Native bradyrhizobia from los Tuxtlas in Mexico are symbionts of Phaseolus lunatus (lima bean). Syst Appl Microbiol 36:33–38CrossRefPubMedGoogle Scholar
  18. Ormeño-Orrillo E, Vinuesa P, Zúñiga-Dávila D, Martínez-Romero E (2006) Molecular diversity of native bradyrhizobia isolated from lima bean (Phaseolus lunatus L.) in Peru. Syst Appl Microbiol 29:253–262CrossRefPubMedGoogle Scholar
  19. Peoples MB, Faizah AW, Rerkasem B, Herridge DF (1989) Methods for evaluating nitrogen fixation by nodulated legumes in the field. Australian Centre for International Agricultural Research, Camberra, Monograph 11, p 76Google Scholar
  20. Santamaría RI, Bustos P, Sepúlveda-Robles O, Lozano L, Rodríguez C, Fernández JL, Juárez S, Kameyama L, Guarneros G, Dávila G, González V (2014) Narrow-host-range bacteriophages that infect Rhizobium etli associate with distinct genomic types. App Environ Microbiol 80:446–454CrossRefGoogle Scholar
  21. Santana MA, Pihakaski-Maunsbach K, Sandal N, Marcker KA, Smith AG (1998) Evidence that the plant host synthesizes the heme moiety of leghemoglobin in root nodules. Plant Physiol 116:1259–1269CrossRefPubMedPubMedCentralGoogle Scholar
  22. Tajima R, Lee ON, Abe J, Lux A, Morita S (2007) Nitrogen-fixing activity of root nodules in relation to their size in peanut (Arachis hypogaea L.). Plant Prod Sci 10:423–429CrossRefGoogle Scholar
  23. Thies JE, Singleton PW, Bohlool BB (1991) Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Appl Environ Microbiol 57:19–28PubMedPubMedCentralGoogle Scholar
  24. Wang D, Yang S, Tang F, Zhu H (2012) Symbiosis specificity in the legume: rhizobial mutualism. Cell Microbiol 14:334–342CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Ademir Sérgio Ferreira de Araujo
    • 1
  • Angela Celis de Almeida Lopes
    • 2
  • Jorge C. Berny Mier y Teran
    • 3
  • Antonia Palkovic
    • 3
  • Paul Gepts
    • 3
  1. 1.Soil Quality Lab., Agricultural Science CenterFederal University of PiauíTeresinaBrazil
  2. 2.Department of Crop Science, Agricultural Science CenterFederal University of PiauíTeresinaBrazil
  3. 3.Department of Plant SciencesUniversity of CaliforniaDavisUSA

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