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Evaluation of antioxidative, analgesic and anti-inflammatory activities of methanolic extract of Myrica nagi leaves - an animal model approach

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Abstract

Myrica nagi (family Myricaceae) is commonly known as Kathphal (Hindi) and Bayberry (English) and it has a long history of usage in traditional medicine. It is popular actinorhizal plant for its symbiotic relationship with Frankia. This study was taken in force to estimate the analgesic, anti-inflammatory and anti-oxidative activities of methanolic extract of Myrica nagi (MMN) in an animal model. Anti-oxidative property of MMN was assessed by free radical scavenging assay (DPPH method). The acute toxicity test of methanolic extract of MMN revealed that the median lethal dose (LD50) was found to be 2080 mg/kg body weight in mice. The anti-inflammatory property was evaluated by carrageenan-induced acute inflammation in rats by measuring rat paw volume at different time intervals and toxicological analysis using mice. The analgesic effect was measured in Wistar rats using the acetic acid-induced writhing test and MMN at 200 mg/kg BW showed 54.56 % inhibition of writhing. MMN showed higher anti-oxidant activity in DPPH assays as compared to standard. High dose of MMN showed a significant reduction (21.71 %) in inflammation after 4 h of treatment, which was comparable to diclofenac (10 mg/kg BW; 32.75 %)-treated group. Significant reduction (p < 0.05) in the levels of inflammatory cytokine (IL-1β and TNF-α) markers were also observed in serum of MMN-treated animals as compared to control. Taken together, the phenolic compounds of MMN may serve as potential herbal drug for amelioration of acute inflammation due to their modulatory action on free radicals.

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Acknowledgments

SKM would like to be grateful to University Grants Commission, New Delhi (Folio number: F.No: 6-537/2015(TG)) for travel grants and Dr. TL Shantha, Director, MLACW for other support. Authors are also thankful to Biotechnology-Finishing School, under Govt. of Karnataka at Maharani Lakshmi Ammanni College for Women for providing animal house facility to perform this study.

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Authors and Affiliations

  1. Department of Biotechnology, Maharani Lakshmi Ammanni College For Women, Bangalore, 560012, India

    Sushil Kumar Middha

  2. Department of Biochemistry, Maharani Lakshmi Ammanni College For Women, Bangalore, 560012, India

    Talambedu Usha

  3. Heymans Institute of Pharmacology, De Pintelaan 185, 9000, Ghent, Belgium

    Dinesh Babu

  4. Department of Botany, NEHU Campus, Shillong, 7930 22, Meghalaya, India

    Arvind Kumar Misra

  5. Department of Biochemistry, CPGS, Jain University, Jayanagar, Bangalore, 560011, India

    Prakash Lokesh

  6. Bamboo Technology, Department of Biotechnology, Bodoland University, Kokrajhar-783370, BTAD, Assam, India

    Arvind Kumar Goyal

Authors
  1. Sushil Kumar Middha
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  2. Talambedu Usha
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  3. Dinesh Babu
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  4. Arvind Kumar Misra
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  5. Prakash Lokesh
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  6. Arvind Kumar Goyal
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Correspondence to Arvind Kumar Goyal.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

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Middha, S.K., Usha, T., Babu, D. et al. Evaluation of antioxidative, analgesic and anti-inflammatory activities of methanolic extract of Myrica nagi leaves - an animal model approach. Symbiosis 70, 179–184 (2016). https://doi.org/10.1007/s13199-016-0422-y

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  • DOI: https://doi.org/10.1007/s13199-016-0422-y

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