Symbiosis

, Volume 66, Issue 2, pp 99–106

Diversity of fungi isolated from three temperate ascidians

  • Susanna López-Legentil
  • Patrick M. Erwin
  • Marta Turon
  • Oded Yarden
Short Communication

Abstract

Ascidians are known to harbor diverse and host-specific bacterial and archaeal communities in their tunic. However, to date, only one ascidian species has been investigated to assess symbiotic relationships with fungi and the extent of their diversity. In this study, we isolated and identified 37 strains of fungi in association with three common ascidian species in the NW Mediterranean Sea: Cystodytes dellechiajei, Didemnum fulgens, and Pycnoclavella communis, and 15 additional strains from concentrated seawater samples collected around the animals. Most of the isolated fungi were classified within four orders: Eurotiales (predominantly Penicillium spp.), Pleosporales, Hypocreales (predominantly Trichoderma spp.), and Capnodiales (Cladosporium spp.). Three additional fungal isolates from C. dellechiajei and D. fulgens belonged to the orders Helotiales, Phylachorales and Microascales, and matched to well-known plant and human pathogens (Botrytis cinerea, Plectosphaerella cucumerina and Scopulariopsis brevicaulis). Host-specificity of ascidian-associated fungi was not apparent and thus the significance of ascidian-fungal associations for ascidian wellbeing and their possible ecological roles remain unknown.

Keywords

Fungus Mediterranean Sea ITS Sea-squirt Tunicata Pathogens 

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Susanna López-Legentil
    • 1
  • Patrick M. Erwin
    • 1
  • Marta Turon
    • 2
  • Oded Yarden
    • 3
  1. 1.Department of Biology and Marine Biology, and Center for Marine ScienceUniversity of North Carolina WilmingtonWilmingtonUSA
  2. 2.Center for Advanced Studies of Blanes (CEAB-CSIC)BlanesSpain
  3. 3.Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and EnvironmentThe Hebrew University of JerusalemRehovotIsrael

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