Skip to main content
Log in

Lateral flow immunoassay-based absolute point-of-care technique for authentication of meat and commercial meat products

  • Original Article
  • Published:
Journal of Food Science and Technology Aims and scope Submit manuscript


Point-of-care (POC) assay is an emerging technique for rapid initial screening of meat fraud incidents in a resource-limited environment. To achieve this goal, a simple extraction protocol is proposed for efficient recovery of meat proteins from raw, heat-processed, and commercial samples as well as meat offals without utilizing sophisticated laboratory settings. A sandwich-format lateral flow immunoassay (LFIA) was developed based on gold nanoparticles as labels and immunoglobulins (IgG and IgY) as biomarkers for meat species identification in raw and cooked meat mixes. The test system showed a sensitivity of 10 ng/mL allowing the detection of as low as 0.063% pork and chicken meat and 0.125% sheep meat (lamb) in meat mixes within 15 min including sample preparation. Reproducibility of the assay was confirmed by the fully consistent intra- and inter-laboratory tests and RT-PCR method. The current study developed a field-deployable extraction technique and highly-specific, sensitive, reproducible, cost-effective, and user-friendly LFIA-based assay for rapid species authentication in raw, cooked, and commercial meat samples and meat offals.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

The datasets generated and/or analysed during the current study is not publicly available due to privacy concerns but are available from the corresponding author upon reasonable request.

Code availability

Not applicable.



Gold nanoparticles


Bovine serum albumin




Immunoglobulin G


Immunoglobulin Y


Lateral flow immunoassay


Limit of detection


Phosphate buffered saline




Real-time polymerase chain reaction


Sodium dodecyl sulfate–polyacrylamide gel electrophoresis


Transmission electron microscopy


Download references


The work was supported by the Department of Biotechnology (DBT), Government of India for financial support (Grant No. BT/PR27198/PFN/20/1333/2017).

Author information

Authors and Affiliations



RB: Conceptualization, Methodology, Investigation, Data Curation, Writing-Original Draft; NBM: Conceptualization, Writing-Review & Editing, Funding Acquisition; SB: Validation, Supervision; VMR: Validation, Data Curation; SB: Supervision, Project Administration; SD: Formal Analysis, Resources, Data Curation; GP and DB: Visualization, Supervision.

Corresponding author

Correspondence to Naveena B Maheswarappa.

Ethics declarations

Conflict of interest

The authors declare that they do not have any conflict of interest.

Consent to participate

Not applicable.

Consent for publication

All authors have approved the manuscript and agree with its submission to Journal of Food Science and Technology.

Ethics approval

This study does not involve any human or animal testing.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Material 1

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Banerjee, R., Maheswarappa, N.B., Biswas, S. et al. Lateral flow immunoassay-based absolute point-of-care technique for authentication of meat and commercial meat products. J Food Sci Technol 60, 772–782 (2023).

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: