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Cannabidiol improves thyroid function via modulating vitamin D3 receptor in vitamin D3 deficiency diet-induced rat model


The study was evaluated the impact of cannabidiol (CBD) on thyroid hormones by modulation cannabinoid receptor-2 (CB2) and vitamin D receptor (VDR) in rats fed with vitamin D3 deficiency diet (VDD). CB2-receptors were analyzed by RT-PCR method and others biomarkers by ELISA. The relative expression of CB2 (thyroid ~ 4 folds), VDR protein (liver, 151.72%), and (kidney, 66%) was significantly increased in CBD-60 compared to VDD. Vitamin D3 metabolites were significantly increased serum (189.42%), kidney (73.84%), and liver (58.11%) in CBD-60 than VDD. Increased thyroxine (59.9%) and calcitonin (213.59%); while decreased thyroid-stimulating hormone (36.15%) and parathyroid hormone (38.64%) was observed CBD treatment in VDD rats. In conclusion, CBD treatment improves CB2 and VDR expression and the level of vitamin D3 metabolites, along with improved thyroid hormones, including calcitonin. This is the first report with an improved CB2 and VDR expression after CBD treatment in VDD induced animals. Thus, CBD can be considered to use in hypothyroidism conditions and to maintain bone health.

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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

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Endocannabinoid system


Vitamin D3 receptor


Cannabinoid receptor


Vitamin D3 deficiency diet


Relative quantification

CT :

Threshold cycle




Thyroid stimulating hormone


Parathyroid hormone


Chronic kidney disease


Mineral and bone disorders


Calcitonin gene-related protein


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The authors extend their sincere thanks and gratitude to Dabur Research Foundation, India, for providing the facilities and support that enabled the successful completion of the work.


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Authors and Affiliations



MKT: Conceptualization, Project administration, Resources, SM: Writing—review & editing, Formal analysis; Software, Methodology, SJ: Conceptualization, Project administration, Supervision, Writing—review & editing, Validation.

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Correspondence to Snehasis Jana.

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The authors declare that they have no competing interests.

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(IAEC/41/506) dated 17th January 2018.

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Trivedi, M.K., Mondal, S. & Jana, S. Cannabidiol improves thyroid function via modulating vitamin D3 receptor in vitamin D3 deficiency diet-induced rat model. J Food Sci Technol 59, 3237–3244 (2022).

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  • Cannabinoid receptor
  • Vitamin D3 deficiency diet
  • Cannabidiol
  • Vitamin D3– metabolite
  • VDR
  • Hypothyroidism