Skip to main content

Cannabidiol improves thyroid function via modulating vitamin D3 receptor in vitamin D3 deficiency diet-induced rat model

Abstract

The study was evaluated the impact of cannabidiol (CBD) on thyroid hormones by modulation cannabinoid receptor-2 (CB2) and vitamin D receptor (VDR) in rats fed with vitamin D3 deficiency diet (VDD). CB2-receptors were analyzed by RT-PCR method and others biomarkers by ELISA. The relative expression of CB2 (thyroid ~ 4 folds), VDR protein (liver, 151.72%), and (kidney, 66%) was significantly increased in CBD-60 compared to VDD. Vitamin D3 metabolites were significantly increased serum (189.42%), kidney (73.84%), and liver (58.11%) in CBD-60 than VDD. Increased thyroxine (59.9%) and calcitonin (213.59%); while decreased thyroid-stimulating hormone (36.15%) and parathyroid hormone (38.64%) was observed CBD treatment in VDD rats. In conclusion, CBD treatment improves CB2 and VDR expression and the level of vitamin D3 metabolites, along with improved thyroid hormones, including calcitonin. This is the first report with an improved CB2 and VDR expression after CBD treatment in VDD induced animals. Thus, CBD can be considered to use in hypothyroidism conditions and to maintain bone health.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Availability of data and material

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Code availability

Not applicable.

Abbreviations

CBD:

Cannabidiol

ECS:

Endocannabinoid system

VDR:

Vitamin D3 receptor

CB1R:

Cannabinoid receptor

VDD:

Vitamin D3 deficiency diet

RQ:

Relative quantification

CT :

Threshold cycle

T4:

Thyroxine

TSH:

Thyroid stimulating hormone

PTH:

Parathyroid hormone

CKD:

Chronic kidney disease

MBD:

Mineral and bone disorders

CGRP:

Calcitonin gene-related protein

References

Download references

Acknowledgements

The authors extend their sincere thanks and gratitude to Dabur Research Foundation, India, for providing the facilities and support that enabled the successful completion of the work.

Funding

Not applicable.

Author information

Authors and Affiliations

Authors

Contributions

MKT: Conceptualization, Project administration, Resources, SM: Writing—review & editing, Formal analysis; Software, Methodology, SJ: Conceptualization, Project administration, Supervision, Writing—review & editing, Validation.

Corresponding author

Correspondence to Snehasis Jana.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Ethics approval

(IAEC/41/506) dated 17th January 2018.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Trivedi, M.K., Mondal, S. & Jana, S. Cannabidiol improves thyroid function via modulating vitamin D3 receptor in vitamin D3 deficiency diet-induced rat model. J Food Sci Technol 59, 3237–3244 (2022). https://doi.org/10.1007/s13197-022-05492-3

Download citation

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13197-022-05492-3

Keywords

  • Cannabinoid receptor
  • Vitamin D3 deficiency diet
  • Cannabidiol
  • Vitamin D3– metabolite
  • VDR
  • Hypothyroidism