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A model system based on glucose–arginine to monitor the properties of Maillard reaction products

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An arginine–glucose mixture (1:2 mol ratio) with pH 9 was heated at 53–100 °C for 10–350 min, and the effects of process parameters were determined during the Maillard reaction (MR). The heating temperature and time were selected as process conditions and were studied with central composite design. The model system was tested based on the values obtained from antioxidant capacity, browning intensity, pH, acrylamide (AC), and hydroxymethylfurfural (HMF) concentrations. Higher temperatures and longer time resulted in higher antioxidant capacity and browning intensity while lowering the pH values. HMF concentration of MR products was found higher in lower temperatures with longer processing time, whereas AC concentration was found higher in high temperatures.

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  • Cha J, Debnath T, Lee K-G (2019) Analysis of α-dicarbonyl compounds and volatiles formed in Maillard reaction model systems. Nat Sci Rep 9:5325

    Article  Google Scholar 

  • Chen K, Zhao J, Shi X, Abdul Q, Jiang Z (2019) Characterization and antioxidant activity of products derived from xylose–bovine casein hydrolysate Maillard reaction: impact of reaction time. Foods 8(7):242

    Article  CAS  Google Scholar 

  • De Vleeschouwer K, Van der Plancken I, Van Loey A, Hendrickx ME (2008) The kinetics of acrylamide formation/elimination in asparagine–glucose systems at different initial reactant concentrations and ratios. Food Chem 111:719–729

    Article  Google Scholar 

  • Delgado-Andrade C, Seiquer I, Haro A et al (2010) Development of the Maillard reaction infoods cooked by different techniques. Intake of Maillard-derived compounds. Food Chem 122:145–153

    Article  CAS  Google Scholar 

  • Gökmen V, Kocadağlı T, Göncüoğlu N, Mogol BA (2012) Model studies on the role of 5-hydroxymethyl-2-furfural in acrylamide formation from asparagine. Food Chem 132:168–174

    Article  Google Scholar 

  • Gu F, Kim JM, Hayat K et al (2009) Characteristics and antioxidant activity of ultrafiltrated Maillard reaction products from a casein–glucose model system. Food Chem 117:48–54

    Article  CAS  Google Scholar 

  • Gu F-L, Kim JM, Abbas S et al (2010) Structure and antioxidant activity of high molecular weight Maillard reaction products from casein–glucose. Food Chem 120:505–511

    Article  CAS  Google Scholar 

  • Han J-R, Zhu Z-M, Wu H-T, Sun N, Tang Y, Yu C-P, Zhao C-C, Zhang Z-Y, Li A-T, Yan J-N (2017) Kinetics of antioxidant-producing Maillard reaction in the mixture of ribose and sea cucumber (Stichopus japonicus) gut hydrolysates. J Aquat Food Prod Technol 26:993–1002

    Article  CAS  Google Scholar 

  • Hemmler D, Gonsior M, Powers LC, Marshall JW, Rychlik M, Taylor AJ, Schmitt-Kopplin P (2019) Simulated sunlight selectively modifies Maillard reaction products in a wide array of chemical reactions. Chem Eur J 25:13208–13217

    Article  CAS  Google Scholar 

  • Huang D, Ou B, Hampsch-Woodill M et al (2002) High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 50:4437–4444

    Article  CAS  Google Scholar 

  • Joint FAO, Organization WH, Additives WHOEC on F (2017) Evaluation of certain contaminants in food: eighty-third report of the Joint FAO/WHO Expert Committee on Food Additives. World Health Organization

  • Knol JJ, van Loon WAM, Linssen JPH et al (2005) Toward a kinetic model for acrylamide formation in a glucose − asparagine reaction system. J Agric Food Chem 53:6133–6139

    Article  CAS  Google Scholar 

  • Knol JJ, Linssen JPH, van Boekel MAJS (2010) Unravelling the kinetics of the formation of acrylamide in the Maillard reaction of fructose and asparagine by multiresponse modelling. Food Chem 120:1047–1057

    Article  CAS  Google Scholar 

  • Kukurová K, Ciesarová Z, Mogol BA et al (2013) Raising agents strongly influence acrylamide and HMF formation in cookies and conditions for asparaginase activity in dough. Eur Food Res Technol 237:1–8

    Article  Google Scholar 

  • Lertittikul W, Benjakul S, Tanaka M (2007) Characteristics and antioxidative activity of Maillard reaction products from a porcine plasma protein–glucose model system as influenced by pH. Food Chem 100:669–677

    Article  CAS  Google Scholar 

  • Liu P, Lu X, Li N, Zheng Z, Qiao X (2019) Characterization, variables, and antioxidant activity of the Maillard reaction in a fructose Histidine model system. Molecules 24:56

    Article  Google Scholar 

  • Liu H, Jiang Y, Guan H, Li F, Sun-Waterhouse D, Chen Y, Li D (2020) Enhancing the antioxidative effects of foods containing rutin and α-amino acids via the Maillard reaction: a model study focusing on rutin–lysine system. J Food Biochem 44:e13086

    PubMed  Google Scholar 

  • Losso JN (2016) The Maillard reaction reconsidered. CRC Press, Boca Raton

    Book  Google Scholar 

  • Mogol BA, Gökmen V (2016) Effect of chitosan on the formation of acrylamide and hydroxymethylfurfural in model, biscuit and crust systems. Food Funct 7:3431–3436

    Article  CAS  Google Scholar 

  • Mogol BA, Yıldırım A, Gökmen V (2010) Inhibition of enzymatic browning in actual food systems by the Maillard reaction products. J Sci Food Agric 90:2556–2562

    Article  CAS  Google Scholar 

  • Morales FJ, Van Boekel M (1997) A study on advanced Maillard reaction in heated casein/sugar solutions: fluorescence accumulation. Int Dairy J 7:675–683

    Article  CAS  Google Scholar 

  • Phisut N, Jiraporn B (2013) Characteristics and antioxidant activity of Maillard reaction products derived from chitosan–sugar solution. Int Food Res J 20:1077

    CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A et al (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  Google Scholar 

  • Rezende YRRS, Nogueira JP, Narain N (2017) Comparison and optimization of conventional and ultrasound assisted extraction for bioactive compounds and antioxidant activity from agro-industrial acerola (Malpighia emarginata DC) residue. LWT Food Sci Technol 85:158–169

    Article  CAS  Google Scholar 

  • Rufian-Henares JA, De la Cueva SP (2008) Assessment of hydroxymethylfurfural intake in the Spanish diet. Food Addit Contam 25:1306–1312

    Article  CAS  Google Scholar 

  • Sánchez-Moreno C (2002) Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 8:121–137

    Article  Google Scholar 

  • Sun Y, Hayakawa S, Chuamanochan M et al (2006a) Antioxidant effects of Maillard reaction products obtained from ovalbumin and different D-aldohexoses. Biosci Biotechnol Biochem 70:598–605

    Article  CAS  Google Scholar 

  • Sun Y, Hayakawa S, Puangmanee S, Izumori K (2006b) Chemical properties and antioxidative activity of glycated α-lactalbumin with a rare sugar, D-allose, by Maillard reaction. Food Chem 95:509–517

    Article  CAS  Google Scholar 

  • Tressl R, Nittka C, Kersten E, Rewicki D (1995) Formation of isoleucine-specific Maillard products from [1-13C]-d-glucose and [1-13C]-D-fructose. J Agric Food Chem 43:1163–1169

    Article  CAS  Google Scholar 

  • Vhangani LN, Van Wyk J (2013) Antioxidant activity of Maillard reaction products (MRPs) derived from fructose–lysine and ribose–lysine model systems. Food Chem 137:92–98

    Article  CAS  Google Scholar 

  • Wedzicha BL, Bellion IR, German G (1994) New Insight into the mechanism of the Maillard reaction from studies of the kinetics of its inhibition by sulfite. In: Labuza TP, Reineccius GA, Monnier VM, O’Brien J, Baynes JW (eds) Maillard reactions in chemistry, food, and health. The Royal Society of Chemistry, London, pp 82–87

    Google Scholar 

  • Wijewickreme AN, Kitts DD, Durance TD (1997) Reaction conditions influence the elementary composition and metal chelating affinity of nondialyzable model Maillard reaction products. J Agric Food Chem 45:4577–4583

    Article  CAS  Google Scholar 

  • Xu H, Zhang X, Karangwa E, Xia S (2017) Correlating enzymatic browning inhibition and antioxidant ability of Maillard reaction products derived from different amino acids. J Sci Food Agric 97:4210–4218

    Article  CAS  Google Scholar 

  • Yu H, Seow Y-X, Ong PKC, Zhou W (2018) Kinetic study of high-intensity ultrasound-assisted Maillard reaction in a model system of d-glucose and glycine. Food Chem 269:628–637

    Article  CAS  Google Scholar 

  • Yu H, Zhong Q, Xie Y, Guo Y, Cheng Y, Yao W (2020) Kinetic study on the generation of furosine and pyrraline in a Maillard reaction model system of d-glucose and l-lysine. Food Chem 317:126458

    Article  CAS  Google Scholar 

  • Zhuang Y, Sun L (2011) Antioxidant activity of maillard reaction products from lysine–glucose model system as related to optical property and copper (II) binding ability. Afr J Biotechnol 10:6784–6793

    CAS  Google Scholar 

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This study was supported by Suleyman Demirel University Scientific Research Projects Coordination Unit (Project # 3380-YL2-12).

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Correspondence to Ece Sogut.

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Sogut, E., Ertekin Filiz, B. & Seydim, A.C. A model system based on glucose–arginine to monitor the properties of Maillard reaction products. J Food Sci Technol 58, 1005–1013 (2021).

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