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Nanoencapsulated Monarda citriodora Cerv. ex Lag. essential oil as potential antifungal and antiaflatoxigenic agent against deterioration of stored functional foods

Abstract

In vitro antifungal activity of the essential oil from Monarda citriodora (MCEO) with possible mode of action was evaluated against A. flavus (AF-LHP-SH1) and 15 other storage molds for controlling postharvest deterioration of stored functional food samples. The chemical profiling of MCEO as done through GC–MS analysis revealed caryophyllene (19.15%) as the major component. The MCEO showed broad spectrum fungitoxicity and completely inhibited the growth of all tested molds and aflatoxin B1 (AFB1) production by AF-LHP-SH1 at 1.40 and 1.20 µL/mL, respectively. Plasma membrane damage and methylglyoxal inhibition was confirmed as the possible antifungal and antiaflatoxigenic mode of action of MCEO. MCEO exhibited remarkable antioxidant activity with IC50 value 2.24 μL/mL as determined through DPPH assay and did not cause adverse effect on seed germination. In addition, the MCEO was encapsulated into chitosan nanoparticle, characterized (SEM, FTIR, XRD) and assessed for their potential against inhibition of growth and AFB1 production. MCEO after encapsulation exhibited enhanced efficacy inhibiting fungal growth and AFB1 production by AF-LHP-SH1 at 0.6 and 0.5 µL/mL, respectively. Encapsulated MCEO may be recommended as novel preservative to extend the shelf life of stored functional food samples.

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Acknowledgement

Deepika is thankful to the University Grants Commission (UGC) [Grant No. F.16-6 (DEC. 2016)/2017] New Delhi, India for financial support. Authors are thankful to Head, CAS in Botany, DST-PURSE, ISLS, Banaras Hindu University, Varanasi for laboratory providing laboratory facility and Indian Institute of Technology, Banaras Hindu University for HR–SEM, FTIR and XRD analysis.

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Correspondence to Nawal Kishore Dubey.

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Deepika, Singh, A., Chaudhari, A.K. et al. Nanoencapsulated Monarda citriodora Cerv. ex Lag. essential oil as potential antifungal and antiaflatoxigenic agent against deterioration of stored functional foods. J Food Sci Technol 57, 2863–2876 (2020). https://doi.org/10.1007/s13197-020-04318-4

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Keywords

  • Monarda citriodora essential oil
  • Functional foods
  • Aflatoxin B1
  • Methylglyoxal
  • Chitosan
  • Nanoencapsulation