Abstract
Ghee, the clarified butter fat being the costliest fat among other edible oils and fats in India, prone to adulteration with highly manipulated cheaper oils/fats, especially during lean season. The present investigation carried on triglycerides profile of one of the latest components of the adulterant fat i.e. RM (Reichert–Meissl)-adjuster, has been exploited to check the adulteration of ghee with a newly emerged highly manipulated fat. Using standardized (S)-limits specified by the ISO/IDF for cow milk fat, the minimum level of detection of the adulterant fat was observed as 7.5%. However, in case of buffalo ghee, due to non-availability of the ISO/IDF limits, the detection of this adulterant fat in buffalo ghee was not possible. Gas chromatograms showed specific signature peaks of large size in the retention time region of 4.5 to 6.5 min for RM-adjuster and adulterant fat, whereas no such peaks were observed in pure cow and buffalo ghee samples. The new approach of zooming in and superimposing of selected peaks in the chromatograms of triglycerides of suspected ghee has been used as a strategy to find adulterant fat’s presence. Through this approach, the addition of RM-adjusted highly manipulated foreign fat (adulterant fat) to the tune of even 0.5% could be achieved in both cow as well as buffalo ghee.
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References
Achaya KT (1997) Ghee, vanaspati and special fats in India. In: Gunstone D, Padley FB (eds) Lipid technologies and applications. Marcel Dekker Inc., New York, pp 369–390
Ayari F, Mirzaee-Ghaleh E, Rabbani H, Heidarbeigi K (2018) Detection of the adulteration in pure cow ghee by electronic nose method (case study: sunflower oil and cow body fat). Int J Food Prop 21(1):1670–1679
FSSR (2011) File no.1 94/FSSAI/SP/ (labelling). Food Safety and Standards Authority of India (Ministry of Health and Family Welfare). FDA Bhavan, Kotla Road New Delhi-110002
GAIN (2014) India Dairy and Products annual. Gain Report No: IN 4089, Recent GAIN Publications. https://gain.fas.usda.gov. Accessed 23 Aug 2019
GAIN (2017) India Dairy and Products annual. Gain Report No: IN 7123, Recent GAIN Publications. https://gain.fas.usda.gov. Accessed 23 Aug 2019
Garg B (2018) Newspaper article: spurious desi ghee seized, 4 held. Posted on October 31, 2018. Tribune news service. https://www.tribuneindia.com/news/bathinda/spurious-desi-ghee-seized-4-held/676237.html. Accessed 16 April 2019
Hazra T, Sharma V, Sharma R, Arora S (2018) Triglyceride profiling of ghee containing goat tallow and admixture of soy oil and goat tallow for detecting such an adulteration. Indian J Dairy Sci 71(2):176–182
IMARC (2019) Ghee market in India: industry trends, share, size, growth, opportunity and forecast 2019–2024. https://www.imarcgroup.com/ghee-market-size-in-india. Accessed 16 April 2019
IS (1981) Handbook of food analysis. Part XI dairy products Bureau of Indian SP: 18 (part XI). Standards, Manak Bhavan, New Delhi
ISO (2010) Milk and milk products—determination of milk fat purity by gas chromatographic analysis of triglycerides. ISO 17678:2010(E). ISO copyright office International Dairy Federation Case postale 56, CH-1211 Geneva
Jirankalgikar NM, De S (2014) Detection of tallow adulteration in cow ghee by derivative spectrophotometry. J Nat Sci Biol Med 5(2):317–319
Kala A (2013) Detection of possible adulteration in commercial ghee samples using low-resolution gas chromatography triglyceride profiles. Int J Dairy Technol 3:346–351
Kala AA, Sabeena K, Havanur PP (2016) Determination of triacyl glycerol and sterol components of fat to authenticate ghee based sweets. J Food Sci Technol 53(4):2144–2147
Kim HJ, Park JM, Lee JH, Kim JM (2016) Detection for non-milk fat in dairy product by gas chromatography. Korean J Food Sci Anim Resour 36(2):206–214
Precht D (1992) Detection of foreign fat in milk fat II. Quantitative evaluation of foreign fat mixtures. Zeitschrift für Lebensmittel-Untersuchung und Forschung 1194(2):107–114.
Ramani A, Hazra T, Sudheendra CVK, Hariyani AS, Prasad S, Ramani VM (2018) Comparative appraisal of ghee and palm oil adulterated ghee on the basis of chromogenic test. Int J Curr Microbiol App Sci 7(12):623–627
Sharma V, Hazra T, Kandhol R, Sharma R, Arora S (2018) Confirmation of buffalo tallow in anhydrous cow milk fat using gas liquid chromatography in tandem with species-specific polymerase chain reaction. Int J Dairy Technol 71(1):158–163
Upadhyay N, Jaiswal P, Jha N (2016) Detection of goat body fat adulteration in pure ghee using ATR-FTIR spectroscopy coupled with chemometrics strategy. J Food Sci Technol 53:3752–7760
Acknowledgements
Authors are thankful to Director of ICAR-National Dairy Research Institute for providing all facilities for carrying out this research. Authors are also thankful to Food Safety and Standards Authority of India for providing the funds to procure Gas Liquid Chromatography machine in the laboratory. Authors are also thankful to Dr Ankit Goyal for the very valuable suggestions during the compilation of the paper. We are also thankful to State Food Laboratory, Dairy Cooperative and Referral Laboratory of NDRI to provide with the suspected samples.
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Pathania, P., Sharma, V., Arora, S. et al. A novel approach to detect highly manipulated fat adulterant as Reichert–Meissl value-adjuster in ghee (clarified butter) through signature peaks by gas chromatography of triglycerides. J Food Sci Technol 57, 191–199 (2020). https://doi.org/10.1007/s13197-019-04047-3
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DOI: https://doi.org/10.1007/s13197-019-04047-3