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Pro-oxidant versus anti-oxidant effects of seeds aglycone extracts of Lepidium sativum and Eruca vesicaria Linn., in vitro, and on neutrophil nitro-oxidative functions

  • Nabila Tounsi
  • Bahia DjerdjouriEmail author
  • Ouahiba Ait Yahia
  • Aicha Belkebir
Original Article
  • 7 Downloads

Abstract

This study evaluated the anti-inflammatory and antioxidant properties of seeds aglycone extracts from Lepidium sativum (LS) and Eruca vesicaria (EV) Linn., on oxidative damages in vitro and on neutrophil nitro-oxidative functions. The results showed that LS and EV aglycone extracts attenuated liver microsomal lipids and proteins oxidation through a potent antioxidant effect as attested by the dose dependent quenching of DPPH radical scavenging activity. LS and EV aglycone extracts inhibited dose dependently the production of superoxide anion by BALB/c mice-derived peritoneal neutrophils, whereas they slightly enhanced exocytosis of myeloperoxidase (MPO), a marker of azurophilic granules. Interestingly, only LS replenished glutathione (GSH) and nitric oxide levels, indicating a fine differential effect. This study highlighted the subtle oxidative and antioxidant capacity of LS and EV seeds aglycone extracts. These health promoting compounds could be used to finely modulate critical events involved in microbial infection, inflammation and nitro-oxidative stress.

Keywords

Eruca vesicaria Lepidium sativum Glutathione Lipid peroxides Myeloperoxidase Nitro-oxidative stress 

Abbreviations

AA

Ascorbic acid

EV

Eruca vesicaria

GSH

Reduced glutathione

HNE

4-Hydroxynonenal

LPI

Lipid peroxidation inhibition

LS

Lepidium sativum

MDA

Malondialdehyde

MPO

Myeloperoxidase

NAC

N-Acetylcysteine

NO

Nitric oxide

NOS

Nitric oxide synthase

NOX2

NADPH oxidase2

ProtCO

Protein carbonyls

PN

Neutrophils

ROS

Reactive oxygen species

O2·−

Superoxide anion

Notes

Acknowledgements

The authors are grateful to Dr Haj-Arab. H. for the identification of the plant

Compliance with ethical standards

Conflict of interest

All authors declare that there is no conflict of interest.

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Copyright information

© Association of Food Scientists & Technologists (India) 2019

Authors and Affiliations

  • Nabila Tounsi
    • 1
  • Bahia Djerdjouri
    • 1
    Email author
  • Ouahiba Ait Yahia
    • 2
  • Aicha Belkebir
    • 2
  1. 1.Laboratory of Cellular and Molecular Biology, Faculty of Biological SciencesUniversity of Sciences and Technology, Houari BoumedieneAlgiersAlgeria
  2. 2.Laboratory of Plant Physiology, Faculty of Biological SciencesUniversity of Sciences and Technology, Houari BoumedieneAlgiersAlgeria

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